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Yorodumi- EMDB-37178: PASR pre-processed Apoferritin (PASR 4K counting mode from 32 Fal... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-37178 | |||||||||
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Title | PASR pre-processed Apoferritin (PASR 4K counting mode from 32 Falcon 4 micrographs) with CryoSPARC | |||||||||
Map data | Post-processed PASR pre-processed 4K Apoferritin (CryoSPARC) | |||||||||
Sample |
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Keywords | PASR / post-acquisition super resolution / apoferritin / METAL BINDING PROTEIN | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.45 Å | |||||||||
Authors | Burton-Smith RN / Murata K | |||||||||
Funding support | Japan, 1 items
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Citation | Journal: To Be Published Title: PASR pre-processed Apoferritin (PASR 4K counting mode from 32 Falcon 4 micrographs) with CryoSPARC Authors: Burton-Smith RN / Murata K | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_37178.map.gz | 59.7 MB | EMDB map data format | |
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Header (meta data) | emd-37178-v30.xml emd-37178.xml | 11.3 KB 11.3 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_37178_fsc.xml | 8.4 KB | Display | FSC data file |
Images | emd_37178.png | 146.6 KB | ||
Filedesc metadata | emd-37178.cif.gz | 3.7 KB | ||
Others | emd_37178_half_map_1.map.gz emd_37178_half_map_2.map.gz | 59.3 MB 59.3 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-37178 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-37178 | HTTPS FTP |
-Validation report
Summary document | emd_37178_validation.pdf.gz | 950.5 KB | Display | EMDB validaton report |
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Full document | emd_37178_full_validation.pdf.gz | 950.1 KB | Display | |
Data in XML | emd_37178_validation.xml.gz | 16.2 KB | Display | |
Data in CIF | emd_37178_validation.cif.gz | 21.2 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-37178 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-37178 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_37178.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Post-processed PASR pre-processed 4K Apoferritin (CryoSPARC) | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.956 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half-map 1 PASR pre-processed 4K Apoferritin (CryoSPARC)
File | emd_37178_half_map_1.map | ||||||||||||
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Annotation | Half-map 1 PASR pre-processed 4K Apoferritin (CryoSPARC) | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half-map 2 PASR pre-processed 4K Apoferritin (CryoSPARC)
File | emd_37178_half_map_2.map | ||||||||||||
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Annotation | Half-map 2 PASR pre-processed 4K Apoferritin (CryoSPARC) | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : PASR pre-processed 4K counting mode Apoferritin (CryoSPARC)
Entire | Name: PASR pre-processed 4K counting mode Apoferritin (CryoSPARC) |
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Components |
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-Supramolecule #1: PASR pre-processed 4K counting mode Apoferritin (CryoSPARC)
Supramolecule | Name: PASR pre-processed 4K counting mode Apoferritin (CryoSPARC) type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 500 KDa |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 25.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |