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Yorodumi- EMDB-37177: Apoferritin (8K super resolution from 32 Falcon 4 micrographs) pr... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-37177 | |||||||||
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Title | Apoferritin (8K super resolution from 32 Falcon 4 micrographs) processed with CryoSPARC | |||||||||
Map data | Post-processed 8K Apoferritin (CryoSPARC) | |||||||||
Sample |
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Keywords | PASR / post-acquisition super resolution / apoferritin / METAL BINDING PROTEIN | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.28 Å | |||||||||
Authors | Burton-Smith RN / Murata K | |||||||||
Funding support | Japan, 1 items
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Citation | Journal: To Be Published Title: Apoferritin (8K super resolution from 32 Falcon 4 micrographs) processed with CryoSPARC Authors: Burton-Smith RN / Murata K | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_37177.map.gz | 59.6 MB | EMDB map data format | |
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Header (meta data) | emd-37177-v30.xml emd-37177.xml | 11.2 KB 11.2 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_37177_fsc.xml | 8.4 KB | Display | FSC data file |
Images | emd_37177.png | 143.9 KB | ||
Filedesc metadata | emd-37177.cif.gz | 3.7 KB | ||
Others | emd_37177_half_map_1.map.gz emd_37177_half_map_2.map.gz | 59.2 MB 59.2 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-37177 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-37177 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_37177.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | Post-processed 8K Apoferritin (CryoSPARC) | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.956 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half-map 1 8K Apoferritin (CryoSPARC)
File | emd_37177_half_map_1.map | ||||||||||||
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Annotation | Half-map 1 8K Apoferritin (CryoSPARC) | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half-map 2 8K Apoferritin (CryoSPARC)
File | emd_37177_half_map_2.map | ||||||||||||
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Annotation | Half-map 2 8K Apoferritin (CryoSPARC) | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : 8K super resolution Apoferritin (CryoSPARC)
Entire | Name: 8K super resolution Apoferritin (CryoSPARC) |
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Components |
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-Supramolecule #1: 8K super resolution Apoferritin (CryoSPARC)
Supramolecule | Name: 8K super resolution Apoferritin (CryoSPARC) / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 500 KDa |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 25.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |