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- EMDB-36931: Cryo-EM reconstruction of the pseudo-helical symmetric tubular as... -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Cryo-EM reconstruction of the pseudo-helical symmetric tubular assembly of SBA/GN8GN at 36.5 nm diameter | |||||||||
![]() | 3D reconstruction of the SBA/GN8GN tubular assembly | |||||||||
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![]() | protein tube / self-assembly / polymorphism / protein-carbohydrate interaction / PLANT PROTEIN | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 12.5 Å | |||||||||
![]() | Chen SY / Zhang L / Chen GS | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM reconstruction of the pseudo-helical symmetric tubular assembly of SBA/GN8GN at 36.5 nm diameter Authors: Chen SY / Zhang L / Chen GS | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 34.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 11.6 KB 11.6 KB | Display Display | ![]() |
Images | ![]() | 52.7 KB | ||
Filedesc metadata | ![]() | 3.6 KB | ||
Others | ![]() ![]() | 194.3 MB 194.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 799.5 KB | Display | ![]() |
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Full document | ![]() | 799.1 KB | Display | |
Data in XML | ![]() | 16 KB | Display | |
Data in CIF | ![]() | 19.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | 3D reconstruction of the SBA/GN8GN tubular assembly | ||||||||||||||||||||
Voxel size | X=Y=Z: 2.051 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: EM half map of the 3D reconstruction of SBA/GN8GN tubular assembly
File | emd_36931_half_map_1.map | ||||||||||||
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Annotation | EM half map of the 3D reconstruction of SBA/GN8GN tubular assembly | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: EM half map of the 3D reconstruction of SBA/GN8GN tubular assembly
File | emd_36931_half_map_2.map | ||||||||||||
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Annotation | EM half map of the 3D reconstruction of SBA/GN8GN tubular assembly | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : The tubular assembly of soybean agglutinin protein with GN8GN ligand
Entire | Name: The tubular assembly of soybean agglutinin protein with GN8GN ligand |
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Components |
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-Supramolecule #1: The tubular assembly of soybean agglutinin protein with GN8GN ligand
Supramolecule | Name: The tubular assembly of soybean agglutinin protein with GN8GN ligand type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | 3D array |
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Sample preparation
Buffer | pH: 7.2 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TALOS ARCTICA |
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Image recording | Film or detector model: FEI CETA (4k x 4k) / Average electron dose: 45.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.4 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 57000 |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: OTHER |
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Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 12.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 84608 |
Initial angle assignment | Type: NOT APPLICABLE |
Final angle assignment | Type: NOT APPLICABLE |