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- EMDB-36892: Structure of BtKY72 spike receptor-binding domain (RBD) complexed... -
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Open data
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Basic information
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Title | Structure of BtKY72 spike receptor-binding domain (RBD) complexed with bat ACE2 | |||||||||
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![]() | BtKY72 RBD ACE2 bat / VIRAL PROTEIN | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||
![]() | Su C / Qi JX / Gao GF | |||||||||
Funding support | 1 items
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![]() | ![]() Title: Structural characteristics of BtKY72 RBD bound to bat ACE2 reveal multiple key residues affecting ACE2 usage of sarbecoviruses Authors: Su C / He JH / Qi JX / Yang MS / Wang QH / Gao GF | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 167.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.4 KB 15.4 KB | Display Display | ![]() |
Images | ![]() | 31 KB | ||
Filedesc metadata | ![]() | 5.6 KB | ||
Others | ![]() ![]() | 165.4 MB 165.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 856.7 KB | Display | ![]() |
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Full document | ![]() | 856.3 KB | Display | |
Data in XML | ![]() | 15 KB | Display | |
Data in CIF | ![]() | 17.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.669 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_36892_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_36892_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Structure of BtKY72 spike receptor-binding domain (RBD) complexed...
Entire | Name: Structure of BtKY72 spike receptor-binding domain (RBD) complexed with bat ACE2 |
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Components |
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-Supramolecule #1: Structure of BtKY72 spike receptor-binding domain (RBD) complexed...
Supramolecule | Name: Structure of BtKY72 spike receptor-binding domain (RBD) complexed with bat ACE2 type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: ![]() ![]() |
-Supramolecule #2: BtKY72 RBD
Supramolecule | Name: BtKY72 RBD / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Supramolecule #3: Bat ACE2
Supramolecule | Name: Bat ACE2 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2 |
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-Macromolecule #1: BtKY72
Macromolecule | Name: BtKY72 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 25.514703 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: RVSPSTEVVR FPNITNLCPF GQVFNASNFP SVYAWERLRI SDCVADYAVL YNSSSSFSTF KCYGVSPTKL NDLCFSSVYA DYFVVKGDD VRQIAPAQTG VIADYNYKLP DDFTGCVLAW NTNSVDSKSG NNFYYRLFRH GKIKPYERDI SNVLYNSAGG T CSSISQLG ...String: RVSPSTEVVR FPNITNLCPF GQVFNASNFP SVYAWERLRI SDCVADYAVL YNSSSSFSTF KCYGVSPTKL NDLCFSSVYA DYFVVKGDD VRQIAPAQTG VIADYNYKLP DDFTGCVLAW NTNSVDSKSG NNFYYRLFRH GKIKPYERDI SNVLYNSAGG T CSSISQLG CYEPLKSYGF TPTVGVGYQP YRVVVLSFEL LNAPATVCGP KKSTELVKNK CVNFHHHHHH |
-Macromolecule #2: ACE2
Macromolecule | Name: ACE2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 69.443156 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: STPEDLAKTF LDDFNSAAEN LSYQSSLASW EYNTNISDEN IQKMDEAGAK WSDFYETQSK HAKNFSLEEI HNDTVKLQLQ ILQQSGSPV LSEDKSKRLN SILNAMSTIY STGKVCRPNN PQECLLLEPG LDNIMGTSKD YNERLWAWEG WRAEVGKQLR P LYEEYVVL ...String: STPEDLAKTF LDDFNSAAEN LSYQSSLASW EYNTNISDEN IQKMDEAGAK WSDFYETQSK HAKNFSLEEI HNDTVKLQLQ ILQQSGSPV LSEDKSKRLN SILNAMSTIY STGKVCRPNN PQECLLLEPG LDNIMGTSKD YNERLWAWEG WRAEVGKQLR P LYEEYVVL KNEMARGYHY EDYGDYWRRD YETEGSPDLE YSRDQLTKDV ERIFAEIKPL YEQLHAYVRA KLMDTYPFHI SP TGCLPAH LLGDMWGRFW TNLYPLTVPF GQKPNIDVTD AMLNQTWDAK RIFKEAEKFF VSIGLPHMTE GFWNNSMLTD PGD GRKVVC HPTAWDLGKG DFRIKMCTKV TMEDFLTAHH EMGHIQYDMA YASQPYLLRN GANEGFHEAV GEVMSLSVAT PKHL KTMGL LSPDFLEDNE TEINFLFKQA LTIVGTLPFT YMLEKWRWMV FKGEIPKEEW MTKWWEMKRK IVGVVEPVPH DETYC DPAS LFHVANDYSF IRYYTRTIFE FQFHEALCRI AKHDGPLHKC DISNSTDAGK KLHQMLSVGK SQPWTSVLKD FVDSKD MDV GPLLRYFEPL YTWLKEQNRN SFVGWNTDWS PHAD |
-Macromolecule #3: ZINC ION
Macromolecule | Name: ZINC ION / type: ligand / ID: 3 / Number of copies: 1 / Formula: ZN |
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Molecular weight | Theoretical: 65.409 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: PDB ENTRY PDB model - PDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 1397414 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |