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Yorodumi- EMDB-36868: Cryo-EM structure of 30S ribosome with cleaved AP-mRNA bound comp... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-36868 | ||||||||||||
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Title | Cryo-EM structure of 30S ribosome with cleaved AP-mRNA bound complex (Body 2) | ||||||||||||
Map data | |||||||||||||
Sample |
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Keywords | 30S Ribosome / AP-mRNA / RIBOSOME | ||||||||||||
Biological species | Escherichia coli K-12 (bacteria) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.52 Å | ||||||||||||
Authors | Ramachandran R / Afsar M / Shukla A | ||||||||||||
Funding support | India, 3 items
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Citation | Journal: To Be Published Title: Cryo-EM structure of 30S ribosome with cleaved AP-mRNA bound complex (Body 2) Authors: Ramachandran R / Afsar M / Shukla A | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_36868.map.gz | 40 MB | EMDB map data format | |
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Header (meta data) | emd-36868-v30.xml emd-36868.xml | 12.2 KB 12.2 KB | Display Display | EMDB header |
Images | emd_36868.png | 114.3 KB | ||
Masks | emd_36868_msk_1.map | 64 MB | Mask map | |
Filedesc metadata | emd-36868.cif.gz | 3.9 KB | ||
Others | emd_36868_half_map_1.map.gz emd_36868_half_map_2.map.gz | 40.3 MB 40.4 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-36868 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-36868 | HTTPS FTP |
-Validation report
Summary document | emd_36868_validation.pdf.gz | 698.9 KB | Display | EMDB validaton report |
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Full document | emd_36868_full_validation.pdf.gz | 698.5 KB | Display | |
Data in XML | emd_36868_validation.xml.gz | 12.4 KB | Display | |
Data in CIF | emd_36868_validation.cif.gz | 14.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-36868 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-36868 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_36868.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.38 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_36868_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_36868_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_36868_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : 30S ribosome complex with AP-mRNA
Entire | Name: 30S ribosome complex with AP-mRNA |
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Components |
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-Supramolecule #1: 30S ribosome complex with AP-mRNA
Supramolecule | Name: 30S ribosome complex with AP-mRNA / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Escherichia coli K-12 (bacteria) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Grid | Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: COUNTING / Average electron dose: 51.41 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: PDB ENTRY PDB model - PDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.52 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 77582 |
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |