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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | mycobacterial efflux pump, AMPPNP bound state | |||||||||
![]() | EM map of mycobacterial efflux pump, AMPPNP bound state | |||||||||
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![]() | ABC transporter / efflux pump / TRANSPORT PROTEIN | |||||||||
Function / homology | ![]() Translocases; Catalysing the translocation of other compounds; Linked to the hydrolysis of a nucleoside triphosphate / response to antibiotic / ATP hydrolysis activity / ATP binding / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.9 Å | |||||||||
![]() | Wang Y / Wu F / Zhang L / Rao Z | |||||||||
Funding support | ![]()
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![]() | ![]() Title: mycobacterial efflux pump, AMPPNP bound state Authors: Wang Y / Wu F / Zhang L / Rao Z | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 203.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 18.2 KB 18.2 KB | Display Display | ![]() |
Images | ![]() | 45.8 KB | ||
Filedesc metadata | ![]() | 6.3 KB | ||
Others | ![]() ![]() | 200.6 MB 200.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8k1oMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | EM map of mycobacterial efflux pump, AMPPNP bound state | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.82 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: half map B of mycobacterial efflux pump, AMPPNP bound state
File | emd_36797_half_map_1.map | ||||||||||||
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Annotation | half map B of mycobacterial efflux pump, AMPPNP bound state | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: half map A of mycobacterial efflux pump, AMPPNP bound state
File | emd_36797_half_map_2.map | ||||||||||||
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Annotation | half map A of mycobacterial efflux pump, AMPPNP bound state | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : ternary complex of an ABC transporter Rv1217c-1218c
Entire | Name: ternary complex of an ABC transporter Rv1217c-1218c |
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Components |
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-Supramolecule #1: ternary complex of an ABC transporter Rv1217c-1218c
Supramolecule | Name: ternary complex of an ABC transporter Rv1217c-1218c / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 120 KDa |
-Macromolecule #1: Multidrug efflux system permease protein Rv1217c
Macromolecule | Name: Multidrug efflux system permease protein Rv1217c / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 56.698039 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MSSTVIDRAR PAGHRAPHRG SGFTGTLGLL RLYLRRDRVS LPLWVLLLSV PLATVYIASV ETVYPDRSAR AAAAAAIMAS PAQRALYGP VYNDSLGAVG IWKAGMFHTL IAVAVILTVI RHTRADEESG RAELIDSTVV GRYANLTGAL LLSFGASIAT G AIGALGLL ...String: MSSTVIDRAR PAGHRAPHRG SGFTGTLGLL RLYLRRDRVS LPLWVLLLSV PLATVYIASV ETVYPDRSAR AAAAAAIMAS PAQRALYGP VYNDSLGAVG IWKAGMFHTL IAVAVILTVI RHTRADEESG RAELIDSTVV GRYANLTGAL LLSFGASIAT G AIGALGLL ATDVAPAGSV AFGVALAASG MVFTAVAAVA AQLSPSARFT RAVAFAVLGT AFALRAIGDA GSGTLSWCSP LG WSLQVRP YAGERWWVLL LSLATAAVLT VLAYRLRAGR DVGAGLIAER PGAGTAGPML SEPFGLAWRL NRGSLLLWTV GLC LYGLVM GSVVHGIGDQ LGDNTAVRDI VTRMGGTGAL EQAFLALAFT MIGMVAAAFA VSLTLRLHQE ETGLRAETLL AGAV SRTHW LASHLAMALA GSAVATLISG VAAGLAYGMT VGDVGGKLPT VVGTAAVQLP AVWLLSAVTV GLFGLAPRFT PVAWG VLVG FIALYLLGSL AGFPQMLLNL EPFAHIPRVG GGDFTAVPLL WLLAIDAALI TLGAMAFRRR DVRC UniProtKB: Multidrug efflux system permease protein Rv1217c |
-Macromolecule #2: Multidrug efflux system ATP-binding protein Rv1218c
Macromolecule | Name: Multidrug efflux system ATP-binding protein Rv1218c / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO EC number: Translocases; Catalysing the translocation of other compounds; Linked to the hydrolysis of a nucleoside triphosphate |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 33.491082 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MSADNHQVPI EIRGLTKHFG SVRALDGLDL TVREGEVHGF LGPNGAGKST TLRILLGLVK ADGGSVRLLG GDPWTDAVDL HRHIAYVPG DVTLWPSLTG GETIDLLARM RGGIDNARRA ELIERFGLDP TKKARTYSKG NRQKVSLISA LSSHATLLLL D EPSSGLDP ...String: MSADNHQVPI EIRGLTKHFG SVRALDGLDL TVREGEVHGF LGPNGAGKST TLRILLGLVK ADGGSVRLLG GDPWTDAVDL HRHIAYVPG DVTLWPSLTG GETIDLLARM RGGIDNARRA ELIERFGLDP TKKARTYSKG NRQKVSLISA LSSHATLLLL D EPSSGLDP LMENVFQQCI GEARQRGVTV LLSSHILAET EALCEKVTII RAGKTVESGS LDALRHLSRT SIKAEMIGDP GD LSQIKGV EDISIEGTTV RAQVDSESLR ELIQVLGHAG VRSLVSQPPT LEELFLRHYS LGPEVAAEQQ VATP UniProtKB: Multidrug efflux system ATP-binding protein Rv1218c |
-Macromolecule #3: CARDIOLIPIN
Macromolecule | Name: CARDIOLIPIN / type: ligand / ID: 3 / Number of copies: 2 / Formula: CDL |
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Molecular weight | Theoretical: 1.464043 KDa |
Chemical component information | ![]() ChemComp-CDL: |
-Macromolecule #4: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
Macromolecule | Name: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / type: ligand / ID: 4 / Number of copies: 2 / Formula: ANP |
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Molecular weight | Theoretical: 506.196 Da |
Chemical component information | ![]() ChemComp-ANP: |
-Macromolecule #5: MAGNESIUM ION
Macromolecule | Name: MAGNESIUM ION / type: ligand / ID: 5 / Number of copies: 2 / Formula: MG |
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Molecular weight | Theoretical: 24.305 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 8 mg/mL |
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Buffer | pH: 7.4 / Details: 150mM NaCl, 20mM Tris, detergent |
Grid | Material: COPPER / Mesh: 300 / Pretreatment - Type: PLASMA CLEANING / Pretreatment - Time: 30 sec. |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 281 K / Instrument: FEI VITROBOT MARK II |
Details | sample mono disperse |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 3629 / Average exposure time: 2.4 sec. / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 70.0 µm / Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.2 µm / Nominal magnification: 29000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |