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Yorodumi- EMDB-36414: Apoferritin at 92% of super resolution sampling limit (200KV CRYO... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-36414 | |||||||||
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Title | Apoferritin at 92% of super resolution sampling limit (200KV CRYOARM + K3 with gold grids) | |||||||||
Map data | Postprocessed map: Apoferritin @ 92% of Super Resolution Nyquist limit (CRYOARM200 K3) | |||||||||
Sample |
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Keywords | Apoferritin / Benchmark / Super resolution / Sampling limit / METAL BINDING PROTEIN | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 1.93 Å | |||||||||
Authors | Burton-Smith RN / Danev R / Murata K | |||||||||
Funding support | Japan, 2 items
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Citation | Journal: To Be Published Title: Apoferritin at 92% of super resolution sampling limit (200KV CRYOARM + K3 with gold grids) Authors: Burton-Smith RN / Danev R / Murata K | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_36414.map.gz | 116.6 MB | EMDB map data format | |
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Header (meta data) | emd-36414-v30.xml emd-36414.xml | 12.9 KB 12.9 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_36414_fsc.xml | 11.2 KB | Display | FSC data file |
Images | emd_36414.png | 190 KB | ||
Others | emd_36414_half_map_1.map.gz emd_36414_half_map_2.map.gz | 93 MB 93 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-36414 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-36414 | HTTPS FTP |
-Validation report
Summary document | emd_36414_validation.pdf.gz | 644 KB | Display | EMDB validaton report |
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Full document | emd_36414_full_validation.pdf.gz | 643.6 KB | Display | |
Data in XML | emd_36414_validation.xml.gz | 18.1 KB | Display | |
Data in CIF | emd_36414_validation.cif.gz | 23.8 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-36414 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-36414 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_36414.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Postprocessed map: Apoferritin @ 92% of Super Resolution Nyquist limit (CRYOARM200 K3) | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.8855 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half map 2: Apoferritin @ 92% of Super...
File | emd_36414_half_map_1.map | ||||||||||||
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Annotation | Half map 2: Apoferritin @ 92% of Super Resolution Nyquist limit (CRYOARM200 K3) | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 1: Apoferritin @ 92% of Super...
File | emd_36414_half_map_2.map | ||||||||||||
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Annotation | Half map 1: Apoferritin @ 92% of Super Resolution Nyquist limit (CRYOARM200 K3) | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Apoferritin
Entire | Name: Apoferritin |
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Components |
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-Supramolecule #1: Apoferritin
Supramolecule | Name: Apoferritin / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 500 KDa |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7 |
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Grid | Material: GOLD / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | JEOL CRYO ARM 200 |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 40.5 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 1.4 mm / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 30000 |
Sample stage | Specimen holder model: JEOL CRYOSPECPORTER |