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- EMDB-36201: Human sodium-dependent vitamin C transporter 1 bound to L-ascorbi... -
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Open data
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Basic information
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Title | Human sodium-dependent vitamin C transporter 1 bound to L-ascorbic acid in an inward-open state | |||||||||
![]() | FSC-weighted, sharpened and masked map by PostProcess | |||||||||
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Function / homology | ![]() nucleobase transport / nucleobase transmembrane transporter activity / L-ascorbate:sodium symporter activity / L-ascorbic acid transmembrane transporter activity / L-ascorbic acid transmembrane transport / dehydroascorbic acid transmembrane transporter activity / dehydroascorbic acid transport / sodium ion transmembrane transporter activity / Vitamin C (ascorbate) metabolism / L-ascorbic acid metabolic process ...nucleobase transport / nucleobase transmembrane transporter activity / L-ascorbate:sodium symporter activity / L-ascorbic acid transmembrane transporter activity / L-ascorbic acid transmembrane transport / dehydroascorbic acid transmembrane transporter activity / dehydroascorbic acid transport / sodium ion transmembrane transporter activity / Vitamin C (ascorbate) metabolism / L-ascorbic acid metabolic process / urate transmembrane transporter activity / intracellular organelle / sodium ion transport / basal plasma membrane / lung development / ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Kobayashi TA / Kusakizako T / Nureki O | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structures of human vitamin C transporter SVCT1 Authors: Kobayashi TA / Kusakizako T / Nureki O | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 2.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 18 KB 18 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.6 KB | Display | ![]() |
Images | ![]() | 112.5 KB | ||
Masks | ![]() | 8 MB | ![]() | |
Filedesc metadata | ![]() | 6.8 KB | ||
Others | ![]() ![]() | 6.7 MB 6.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8jewMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | FSC-weighted, sharpened and masked map by PostProcess | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.996 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Density Histograms |
-Half map: #2
File | emd_36201_half_map_1.map | ||||||||||||
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Density Histograms |
-Half map: #1
File | emd_36201_half_map_2.map | ||||||||||||
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Density Histograms |
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Sample components
+Entire : Human SVCT1 dimer in a substrate-bound inward-open state
+Supramolecule #1: Human SVCT1 dimer in a substrate-bound inward-open state
+Macromolecule #1: Solute carrier family 23 member 1
+Macromolecule #2: ASCORBIC ACID
+Macromolecule #3: SODIUM ION
+Macromolecule #4: Lauryl Maltose Neopentyl Glycol
+Macromolecule #5: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
+Macromolecule #6: CHOLESTEROL
+Macromolecule #7: 2-acetamido-2-deoxy-beta-D-glucopyranose
+Macromolecule #8: PALMITIC ACID
+Macromolecule #9: water
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 7 |
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Grid | Model: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Specialist optics | Energy filter - Name: GIF Bioquantum / Energy filter - Slit width: 25 eV |
Sample stage | Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 3753 / Average electron dose: 49.7 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |