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- EMDB-36141: The cryo-EM structure of Paeniclostridium sordellii lethal toxin ... -

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Basic information

Entry
Database: EMDB / ID: EMD-36141
TitleThe cryo-EM structure of Paeniclostridium sordellii lethal toxin (TcsL)
Map data
Sample
  • Complex: The TcsL with InsP6
    • Protein or peptide: Cytotoxin-L
  • Ligand: ZINC ION
KeywordsPaeniclostridium sordellii / TcsL InsP6 / TOXIN
Function / homology
Function and homology information


host cell cytosol / Transferases; Glycosyltransferases; Hexosyltransferases / glycosyltransferase activity / cysteine-type peptidase activity / host cell endosome membrane / toxin activity / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / lipid binding / host cell plasma membrane / proteolysis ...host cell cytosol / Transferases; Glycosyltransferases; Hexosyltransferases / glycosyltransferase activity / cysteine-type peptidase activity / host cell endosome membrane / toxin activity / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / lipid binding / host cell plasma membrane / proteolysis / extracellular region / membrane / metal ion binding
Similarity search - Function
Dermonecrotic/RTX toxin, membrane localization domain / Membrane Localization Domain / TcdA/TcdB toxin, N-terminal helical domain / TcdB toxin N-terminal helical domain / TcdA/TcdB toxin, catalytic glycosyltransferase domain / TcdA/TcdB catalytic glycosyltransferase domain / TcdA/TcdB toxin, pore forming domain / TcdA/TcdB pore forming domain / CGT/MARTX, cysteine protease (CPD) domain / CGT/MARTX, cysteine protease (CPD) domain superfamily ...Dermonecrotic/RTX toxin, membrane localization domain / Membrane Localization Domain / TcdA/TcdB toxin, N-terminal helical domain / TcdB toxin N-terminal helical domain / TcdA/TcdB toxin, catalytic glycosyltransferase domain / TcdA/TcdB catalytic glycosyltransferase domain / TcdA/TcdB toxin, pore forming domain / TcdA/TcdB pore forming domain / CGT/MARTX, cysteine protease (CPD) domain / CGT/MARTX, cysteine protease (CPD) domain superfamily / Peptidase C80 family / CGT/MARTX cysteine protease (CPD) domain profile. / Choline-binding repeat / Putative cell wall binding repeat / Cell wall/choline-binding repeat / Cell wall-binding repeat profile. / Nucleotide-diphospho-sugar transferases
Similarity search - Domain/homology
Biological speciesPaeniclostridium sordellii (strain ATCC 9714 / DSM 2141 / JCM 3814 / LMG 15708 / NCIMB 10717 / 211) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsZhan X / Tao L
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: Nat Commun / Year: 2023
Title: Structural dynamics of CROPs control stability and toxicity of Paeniclostridium sordellii lethal toxin
Authors: Yao Z / Xiechao Z / Liang T
History
DepositionMay 8, 2023-
Header (metadata) releaseDec 13, 2023-
Map releaseDec 13, 2023-
UpdateDec 20, 2023-
Current statusDec 20, 2023Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_36141.png

Downloads & links

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Map

FileDownload / File: emd_36141.map.gz / Format: CCP4 / Size: 98.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.09 Å/pix.
x 296 pix.
= 321.752 Å		1.09 Å/pix.
x 296 pix.
= 321.752 Å		1.09 Å/pix.
x 296 pix.
= 321.752 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.087 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.02
Minimum - Maximum-0.09894645 - 0.2043317
Average (Standard dev.)-0.000014409805 (±0.004536078)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions296296296
Spacing296296296
CellA=B=C: 321.752 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_36141_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_36141_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : The TcsL with InsP6

EntireName: The TcsL with InsP6
Components
  • Complex: The TcsL with InsP6
    • Protein or peptide: Cytotoxin-L
  • Ligand: ZINC ION

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Supramolecule #1: The TcsL with InsP6

SupramoleculeName: The TcsL with InsP6 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Paeniclostridium sordellii (strain ATCC 9714 / DSM 2141 / JCM 3814 / LMG 15708 / NCIMB 10717 / 211) (bacteria)

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Macromolecule #1: Cytotoxin-L

MacromoleculeName: Cytotoxin-L / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
EC number: Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases
Source (natural)Organism: Paeniclostridium sordellii (strain ATCC 9714 / DSM 2141 / JCM 3814 / LMG 15708 / NCIMB 10717 / 211) (bacteria)
Molecular weightTheoretical: 271.827531 KDa
Recombinant expressionOrganism: Bacillus subtilis (bacteria)
SequenceString: MNLVNKAQLQ KMAYVKFRIQ EDEYVAILNA LEEYHNMSES SVVEKYLKLK DINNLTDNYL NTYKKSGRNK ALKKFKEYLT MEVLELKNN SLTPVEKNLH FIWIGGQIND TAINYINQWK DVNSDYTVKV FYDSNAFLIN TLKKTIVESA TNNTLESFRE N LNDPEFDY ...String:
MNLVNKAQLQ KMAYVKFRIQ EDEYVAILNA LEEYHNMSES SVVEKYLKLK DINNLTDNYL NTYKKSGRNK ALKKFKEYLT MEVLELKNN SLTPVEKNLH FIWIGGQIND TAINYINQWK DVNSDYTVKV FYDSNAFLIN TLKKTIVESA TNNTLESFRE N LNDPEFDY NKFYRKRMEI IYDKQKHFID YYKSQIEENP EFIIDNIIKT YLSNEYSKDL EALNKYIEES LNKITANNGN DI RNLEKFA DEDLVRLYNQ ELVERWNLAA ASDILRISML KEDGGVYLDV DMLPGIQPDL FKSINKPDSI TNTSWEMIKL EAI MKYKEY IPGYTSKNFD MLDEEVQRSF ESALSSKSDK SEIFLPLDDI KVSPLEVKIA FANNSVINQA LISLKDSYCS DLVI NQIKN RYKILNDNLN PSINEGTDFN TTMKIFSDKL ASISNEDNMM FMIKITNYLK VGFAPDVRST INLSGPGVYT GAYQD LLMF KDNSTNIHLL EPELRNFEFP KTKISQLTEQ EITSLWSFNQ ARAKSQFEEY KKGYFEGALG EDDNLDFAQN TVLDKD YVS KKILSSMKTR NKEYIHYIVQ LQGDKISYEA SCNLFSKDPY SSILYQKNIE GSETAYYYSV ADAEIKEIDK YRIPYQI SN KRKIKLTFIG HGKSEFNTDT FANLDVDSLS SEIETILNLA KADISPKYIE INLLGCNMFS YSISAEETYP GKLLLKIK D RVSELMPSIS QDSITVSANQ YEVRINEEGK REILDHSGKW INKEESIIKD ISSKEYISFN PKENKIIVKS KYLHELSTL LQEIRNNANS SDIDLEKKVM LTECEINVAS NIDRQIVEGR IEEAKNLTSD SINYIKNEFK LIESISDSLY DLKHQNGLDD SHFISFEDI SKTENGFRIR FINKETGNSI FIETEKEIFS EYATHISKEI SNIKDTIFDN VNGKLVKKVN LDAAHEVNTL N SAFFIQSL IEYNTTKESL SNLSVAMKVQ VYAQLFSTGL NTITDASKVV ELVSTALDET IDLLPTLSEG LPIIATIIDG VS LGAAIKE LSETNDPLLR QEIEAKIGIM AVNLTAASTA IVTSALGIAS GFSILLVPLA GISAGIPSLV NNELILQDKA TKV IDYFKH ISLAETEGAF TLLDDKIIMP QDDLVLSEID FNNNSITLGK CEIWRAEGGS GHTLTDDIDH FFSSPSITYR KPWL SIYDV LNIKKEKIDF SKDLMVLPNA PNRVFGYEMG WTPGFRSLDN DGTKLLDRIR DHYEGQFYWR YFAFIADALI TKLKP RYED TNVRINLDGN TRSFIVPVIT TEQIRKNLSY SFYGSGGSYS LSLSPYNMNI DLNLVENDTW VIDVDNVVKN ITIESD EIQ KGELIENILS KLNIEDNKII LNNHTINFYG DINESNRFIS LTFSILEDIN IIIEIDLVSK SYKILLSGNC MKLIENS SD IQQKIDHIGF NGEHQKYIPY SYIDNETKYN GFIDYSKKEG LFTAEFSNES IIRNIYMPDS NNLFIYSSKD LKDIRIIN K GDVKLLIGNY FKDDMKVSLS FTIEDTNTIK LNGVYLDENG VAQILKFMNN AKSALNTSNS LMNFLESINI KNIFYNNLD PNIEFILDTN FIISGSNSIG QFELICDKDK NIQPYFIKFK IKETSYTLYV GNRQNLIVEP SYHLDDSGNI SSTVINFSQK YLYGIDRYV NKVIIAPNLY TDEINITPVY KPNYICPEVI ILDANYINEK INVNINDLSI RYVWDNDGSD LILIANSEED N QPQVKIRF VNVFKSDTAA DKLSFNFSDK QDVSVSKIIS TFSLAAYSDG FFDYEFGLVS LDNDYFYINS FGNMVSGLIY IN DSLYYFK PPKNNLITGF TTIDGNKYYF DPTKSGAASI GEITIDGKDY YFNKQGILQV GVINTSDGLK YFAPAGTLDE NLE GESVNF IGKLNIDGKI YYFEDNYRAA VEWKLLDDET YYFNPKTGEA LKGLHQIGDN KYYFDDNGIM QTGFITINDK VFYF NNDGV MQVGYIEVNG KYFYFGKNGE RQLGVFNTPD GFKFFGPKDD DLGTEEGELT LYNGILNFNG KIYFFDISNT AVVGW GTLD DGSTYYFDDN TAEACIGLTV INDCKYYFDD NGIRQLGFIT INDNIFYFSE SGKIELGYQN INGNYFYIDE SGLVLI GVF DTPDGYKYFA PLNTVNDNIY GQAVKYSGLV RVNEDVYYFG ETYKIETGWI ENETDKYYFD PETKKAYKGI NVVDDIK YY FDENGIMRTG LISFENNNYY FNEDGKMQFG YLNIKDKMFY FGKDGKMQIG VFNTPDGFKY FAHQNTLDEN FEGESINY T GWLDLDGKRY YFTDEYIAAT GSLTIDGYNY YFDPDTAELV VSEHHHHHHH H

UniProtKB: Cytotoxin-L

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Macromolecule #2: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 2 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 229156
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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