ジャーナル: Nat Commun / 年: 2024 タイトル: In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography. 著者: Weimin Li / Angdi Li / Bing Yu / Xiaoxiao Zhang / Xiaoyan Liu / Kate L White / Raymond C Stevens / Wolfgang Baumeister / Andrej Sali / Marion Jasnin / Liping Sun / 要旨: Actin mediates insulin secretion in pancreatic β-cells through remodeling. Hampered by limited resolution, previous studies have offered an ambiguous depiction as depolymerization and ...Actin mediates insulin secretion in pancreatic β-cells through remodeling. Hampered by limited resolution, previous studies have offered an ambiguous depiction as depolymerization and repolymerization. We report the in situ structure of actin remodeling in INS-1E β-cells during glucose-stimulated insulin secretion at nanoscale resolution. After remodeling, the actin filament network at the cell periphery exhibits three marked differences: 12% of actin filaments reorient quasi-orthogonally to the ventral membrane; the filament network mainly remains as cell-stabilizing bundles but partially reconfigures into a less compact arrangement; actin filaments anchored to the ventral membrane reorganize from a "netlike" to a "blooming" architecture. Furthermore, the density of actin filaments and microtubules around insulin secretory granules decreases, while actin filaments and microtubules become more densely packed. The actin filament network after remodeling potentially precedes the transport and release of insulin secretory granules. These findings advance our understanding of actin remodeling and its role in glucose-stimulated insulin secretion.
ダウンロード / ファイル: emd_35894.map.gz / 形式: CCP4 / 大きさ: 864 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
ボクセルのサイズ
X=Y=Z: 12.112 Å
密度
最小 - 最大
-1.469203 - 0.559713
平均 (標準偏差)
0.0022070406 (±0.036216218)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
1024
1024
216
Spacing
1024
1024
216
セル
A: 12402.688 Å / B: 12402.688 Å / C: 2616.1921 Å α=β=γ: 90.0 °
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添付データ
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試料の構成要素
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全体 : INS-1E
全体
名称: INS-1E
要素
細胞: INS-1E
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超分子 #1: INS-1E
超分子
名称: INS-1E / タイプ: cell / ID: 1 / 親要素: 0
由来(天然)
生物種: Rattus (ネズミ)
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実験情報
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構造解析
手法
クライオ電子顕微鏡法
解析
電子線トモグラフィー法
試料の集合状態
cell
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試料調製
緩衝液
pH: 7.45
凍結
凍結剤: ETHANE
切片作成
集束イオンビーム - 装置: OTHER / 集束イオンビーム - イオン: OTHER / 集束イオンビーム - 電圧: 30 / 集束イオンビーム - 電流: 0.5 / 集束イオンビーム - 時間: 30 / 集束イオンビーム - 温度: 78 K / 集束イオンビーム - Initial thickness: 1000 / 集束イオンビーム - 最終 厚さ: 200 集束イオンビーム - 詳細: The value given for _em_focused_ion_beam.instrument is FEI Aquilos2. This is not in a list of allowed values {'DB235', 'OTHER'} so OTHER is written into the XML file.
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電子顕微鏡法
顕微鏡
FEI TITAN
撮影
フィルム・検出器のモデル: FEI FALCON IV (4k x 4k) 平均電子線量: 2.0 e/Å2