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Yorodumi- EMDB-35582: Cryo-EM structure of Cas0740-crRNA-DNAcomplex at 3.3 Angstroms re... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-35582 | |||||||||
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Title | Cryo-EM structure of Cas0740-crRNA-DNAcomplex at 3.3 Angstroms resolution | |||||||||
Map data | ||||||||||
Sample |
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Keywords | CRISPR-Cas0740 / RNA BINDING PROTEIN/RNA/DNA / RNA BINDING PROTEIN-RNA-DNA complex | |||||||||
Biological species | unidentified (others) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.3 Å | |||||||||
Authors | Zhang X / Duan Z / Zhu J | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: Cryo-EM structure of Cas0740-crRNA-DNAcomplex at 3.3 Angstroms resolution Authors: Xi Z / Zhiqiang D / Jiankang Z | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_35582.map.gz | 25.5 MB | EMDB map data format | |
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Header (meta data) | emd-35582-v30.xml emd-35582.xml | 10.8 KB 10.8 KB | Display Display | EMDB header |
Images | emd_35582.png | 102.1 KB | ||
Masks | emd_35582_msk_1.map | 27 MB | Mask map | |
Filedesc metadata | emd-35582.cif.gz | 3.6 KB | ||
Others | emd_35582_half_map_1.map.gz emd_35582_half_map_2.map.gz | 25.1 MB 25.1 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-35582 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-35582 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_35582.map.gz / Format: CCP4 / Size: 27 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.32 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_35582_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_35582_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_35582_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Ternary complex of cas0740 with crRNA and target DNA
Entire | Name: Ternary complex of cas0740 with crRNA and target DNA |
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Components |
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-Supramolecule #1: Ternary complex of cas0740 with crRNA and target DNA
Supramolecule | Name: Ternary complex of cas0740 with crRNA and target DNA / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: unidentified (others) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 5.0 µm / Nominal defocus min: 1.2 µm |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: NONE |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 94145 |