+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-35192 | |||||||||
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Title | A cryoEM structure of the dimer of (S)-carbonyl reductase II | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Dimer / cryoEM / dynamic / OXIDOREDUCTASE | |||||||||
Biological species | Candida parapsilosis (yeast) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||
Authors | Li YH / Wang C / Zhang RZ / Xu Y / Hunt JF | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: A cryoEM structure of the dimer of (S)-carbonyl reductase II Authors: Li YH / Wang C / Zhang RZ / Xu Y / Hunt JF | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_35192.map.gz | 59.7 MB | EMDB map data format | |
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Header (meta data) | emd-35192-v30.xml emd-35192.xml | 11.1 KB 11.1 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_35192_fsc.xml | 11.8 KB | Display | FSC data file |
Images | emd_35192.png | 32.5 KB | ||
Filedesc metadata | emd-35192.cif.gz | 3.6 KB | ||
Others | emd_35192_half_map_1.map.gz emd_35192_half_map_2.map.gz | 59.5 MB 59.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-35192 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-35192 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_35192.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.83 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_35192_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_35192_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : carbonyl reductase
Entire | Name: carbonyl reductase |
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Components |
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-Supramolecule #1: carbonyl reductase
Supramolecule | Name: carbonyl reductase / type: organelle_or_cellular_component / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Candida parapsilosis (yeast) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 6 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.17 µm / Nominal defocus min: 1.37 µm / Nominal magnification: 105000 |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 56.15 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |