+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-35097 | |||||||||
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Title | Tomogram of apical part of Toxoplasma gondii tachyzoite | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Toxoplasma gondii / tachyzoite / conoid / CELL INVASION | |||||||||
Biological species | Toxoplasma gondii RH (eukaryote) | |||||||||
Method | electron tomography / cryo EM | |||||||||
Authors | Li Z / Guo Q | |||||||||
Funding support | 1 items
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Citation | Journal: Adv Sci (Weinh) / Year: 2023 Title: Cryo-Electron Tomography of Toxoplasma gondii Indicates That the Conoid Fiber May Be Derived from Microtubules. Authors: Zhixun Li / Wenjing Du / Jiong Yang / De-Hua Lai / Zhao-Rong Lun / Qiang Guo / Abstract: Toxoplasma gondii (T. gondii) is the causative agent of toxoplasmosis and can infect numerous warm-blooded animals. An improved understanding of the fine structure of this parasite can help elucidate ...Toxoplasma gondii (T. gondii) is the causative agent of toxoplasmosis and can infect numerous warm-blooded animals. An improved understanding of the fine structure of this parasite can help elucidate its replication mechanism. Previous studies have resolved the ultrastructure of the cytoskeleton using purified samples, which eliminates their cellular context. Here the application of cryo-electron tomography to visualize T. gondii tachyzoites in their native state is reported. The fine structure and cellular distribution of the cytoskeleton are resolved and analyzed at nanometer resolution. Additionally, the tachyzoite structural characteristics are annotated during its endodyogeny for the first time. By comparing the structural features in mature tachyzoites and their daughter buds, it is proposed that the conoid fiber of the Apicomplexa originates from microtubules. This work represents the detailed molecular anatomy of T. gondii, particularly during the budding replication stage of tachyzoite, and provides a reference for further studies of this fascinating organism. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_35097.map.gz | 227.9 MB | EMDB map data format | |
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Header (meta data) | emd-35097-v30.xml emd-35097.xml | 7.5 KB 7.5 KB | Display Display | EMDB header |
Images | emd_35097.png | 246.7 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-35097 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-35097 | HTTPS FTP |
-Validation report
Summary document | emd_35097_validation.pdf.gz | 449.5 KB | Display | EMDB validaton report |
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Full document | emd_35097_full_validation.pdf.gz | 449 KB | Display | |
Data in XML | emd_35097_validation.xml.gz | 4.7 KB | Display | |
Data in CIF | emd_35097_validation.cif.gz | 5.3 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-35097 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-35097 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_35097.map.gz / Format: CCP4 / Size: 246.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. generated in cubic-lattice coordinate | ||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 26.624 Å | ||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : Toxoplasma gondii tachyzoite (apical part)
Entire | Name: Toxoplasma gondii tachyzoite (apical part) |
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Components |
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-Supramolecule #1: Toxoplasma gondii tachyzoite (apical part)
Supramolecule | Name: Toxoplasma gondii tachyzoite (apical part) / type: cell / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Toxoplasma gondii RH (eukaryote) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | electron tomography |
Aggregation state | cell |
-Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
Sectioning | Focused ion beam - Instrument: OTHER / Focused ion beam - Ion: OTHER / Focused ion beam - Voltage: 30 / Focused ion beam - Current: 0.5 / Focused ion beam - Duration: 1800 / Focused ion beam - Temperature: 100 K / Focused ion beam - Initial thickness: 1000 / Focused ion beam - Final thickness: 180 Focused ion beam - Details: The value given for _em_focused_ion_beam.instrument is Aquilos. This is not in a list of allowed values {'OTHER', 'DB235'} so OTHER is written into the XML file. |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 3.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 5.0 µm / Nominal defocus min: 3.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Number images used: 38 |
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