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- EMDB-35069: Cryo-EM structure of delta N15 MsDps2 of Mycobacterium smegmatis -
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Open data
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Basic information
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Title | Cryo-EM structure of delta N15 MsDps2 of Mycobacterium smegmatis | |||||||||
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![]() | Cryo-EM / N15MsDps2 / DNA BINDING PROTEIN | |||||||||
Function / homology | ![]() oxidoreductase activity, acting on metal ions / ferric iron binding / DNA binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.56 Å | |||||||||
![]() | Garg P / Dutta S | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structure of delta N15 MsDps2 of Mycobacterium smegmatis Authors: Garg P / Dutta S | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 49.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.9 KB 12.9 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 7.9 KB | Display | ![]() |
Images | ![]() | 89.1 KB | ||
Filedesc metadata | ![]() | 4.9 KB | ||
Others | ![]() ![]() | 48.9 MB 48.9 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 16 KB | Display | |
Data in CIF | ![]() | 20.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8hwzMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.92 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_35069_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_35069_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Cryo-EM structure of delta N15 MsDps2 of Mycobacterium smegmatis
Entire | Name: Cryo-EM structure of delta N15 MsDps2 of Mycobacterium smegmatis |
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Components |
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-Supramolecule #1: Cryo-EM structure of delta N15 MsDps2 of Mycobacterium smegmatis
Supramolecule | Name: Cryo-EM structure of delta N15 MsDps2 of Mycobacterium smegmatis type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Starvation-inducible DNA-binding protein or fine tangled pili maj...
Macromolecule | Name: Starvation-inducible DNA-binding protein or fine tangled pili major subunit type: protein_or_peptide / ID: 1 / Number of copies: 12 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 16.163995 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: TPEFGGNLQK VLVDLIELSL QGKQAHWNVV GSNFRDLHLQ LDELVDFARE GSDTIAERMR ALDAVPDGRS DTVAATTTLP EFPAFERST ADVVDLITTR INATVDTIRR VHDAVDAEDP STADLLHGLI DGLEKQAWLI RSENRKV UniProtKB: Starvation-inducible DNA-binding protein or fine tangled pili major subunit |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TALOS ARCTICA |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.25 µm / Nominal defocus min: 0.75 µm |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Refinement | Space: REAL / Protocol: RIGID BODY FIT |
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Output model | ![]() PDB-8hwz: |