+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-35019 | |||||||||
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Title | MAVS fibrils surrounding the mitochondria | |||||||||
Map data | representative tomogram of 293T mCherry-MAVS overexpression sample | |||||||||
Sample |
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Keywords | innate immunity / fibrils / IMMUNE SYSTEM | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | electron tomography / cryo EM | |||||||||
Authors | Wang P / Guo Q | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: MAVS fibrils surrounding the mitochondria Authors: Wang P / Guo Q | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_35019.map.gz | 154.3 MB | EMDB map data format | |
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Header (meta data) | emd-35019-v30.xml emd-35019.xml | 9.1 KB 9.1 KB | Display Display | EMDB header |
Images | emd_35019.png | 240.7 KB | ||
Filedesc metadata | emd-35019.cif.gz | 3.4 KB | ||
Others | emd_35019_additional_1.map.gz | 131.4 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-35019 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-35019 | HTTPS FTP |
-Validation report
Summary document | emd_35019_validation.pdf.gz | 589.3 KB | Display | EMDB validaton report |
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Full document | emd_35019_full_validation.pdf.gz | 588.9 KB | Display | |
Data in XML | emd_35019_validation.xml.gz | 5 KB | Display | |
Data in CIF | emd_35019_validation.cif.gz | 5.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-35019 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-35019 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_35019.map.gz / Format: CCP4 / Size: 169.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | representative tomogram of 293T mCherry-MAVS overexpression sample | ||||||||||||||||||||
Voxel size | X=Y=Z: 27.488 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: representative tomogram of 293T mCherry-MAVS-KI sample
File | emd_35019_additional_1.map | ||||||||||||
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Annotation | representative tomogram of 293T mCherry-MAVS-KI sample | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : MAVS fibrils in situ
Entire | Name: MAVS fibrils in situ |
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Components |
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-Supramolecule #1: MAVS fibrils in situ
Supramolecule | Name: MAVS fibrils in situ / type: cell / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Homo sapiens (human) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | electron tomography |
Aggregation state | cell |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
Sectioning | Focused ion beam - Instrument: OTHER / Focused ion beam - Ion: OTHER / Focused ion beam - Voltage: 30 / Focused ion beam - Current: 1 / Focused ion beam - Duration: 0.5 / Focused ion beam - Temperature: 83 K / Focused ion beam - Initial thickness: 10000 / Focused ion beam - Final thickness: 200 Focused ion beam - Details: The value given for _em_focused_ion_beam.instrument is Aquilos 2. This is not in a list of allowed values {'OTHER', 'DB235'} so OTHER is written into the XML file. |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 110.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 7.0 µm / Nominal defocus min: 5.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Number images used: 60 |
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