+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | MAVS fibrils surrounding the mitochondria | |||||||||
![]() | representative tomogram of 293T mCherry-MAVS overexpression sample | |||||||||
![]() |
| |||||||||
![]() | innate immunity / fibrils / IMMUNE SYSTEM | |||||||||
Biological species | ![]() | |||||||||
Method | electron tomography / cryo EM | |||||||||
![]() | Wang P / Guo Q | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: MAVS fibrils surrounding the mitochondria Authors: Wang P / Guo Q | |||||||||
History |
|
-
Structure visualization
-
Downloads & links
-EMDB archive
Map data | ![]() | |||
---|---|---|---|---|
Header (meta data) | ![]() | |||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 589.3 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 588.9 KB | Display | |
Data in XML | ![]() | 5 KB | Display | |
Data in CIF | ![]() | 5.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | representative tomogram of 293T mCherry-MAVS overexpression sample | ||||||||||||||||||||
Voxel size | X=Y=Z: 27.488 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-
Sample components
-Entire : MAVS fibrils in situ
Entire | Name: MAVS fibrils in situ |
---|---|
Components |
|
-Supramolecule #1: MAVS fibrils in situ
Supramolecule | Name: MAVS fibrils in situ / type: cell / ID: 1 / Parent: 0 |
---|---|
Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | electron tomography |
Aggregation state | cell |
-
Sample preparation
Buffer | pH: 7.4 |
---|---|
Vitrification | Cryogen name: ETHANE |
Sectioning | Focused ion beam - Instrument: OTHER / Focused ion beam - Ion: OTHER / Focused ion beam - Voltage: 30 / Focused ion beam - Current: 1 / Focused ion beam - Duration: 0.5 / Focused ion beam - Temperature: 83 K / Focused ion beam - Initial thickness: 10000 / Focused ion beam - Final thickness: 200 Focused ion beam - Details: The value given for _em_focused_ion_beam.instrument is Aquilos 2. This is not in a list of allowed values {'OTHER', 'DB235'} so OTHER is written into the XML file. |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 110.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 7.0 µm / Nominal defocus min: 5.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
-
Image processing
Final reconstruction | Number images used: 60 |
---|