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Open data
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Basic information
Entry | ![]() | ||||||||||||
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Title | Cryo-EM structure of the CnCas12f1-sgRNA-DNA complex | ||||||||||||
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![]() | Complex / CRISPR-Cas / RNA BINDING PROTEIN/RNA/DNA / RNA BINDING PROTEIN-RNA-DNA complex | ||||||||||||
Function / homology | Transposase IS605, OrfB, C-terminal / Putative transposase DNA-binding domain / transposition / DNA recombination / DNA binding / Transposase![]() | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | single particle reconstruction / Resolution: 3.73 Å | ||||||||||||
![]() | Li F / Ji Q | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure and engineering of miniature Clostridium novyi CRISPR-Cas12f1 with rare C-rich PAM specificity Authors: Li F / Ji Q | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 203.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.4 KB 15.4 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 14.3 KB | Display | ![]() |
Images | ![]() | 111.3 KB | ||
Others | ![]() ![]() | 200.7 MB 200.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8hr5MC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.82 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_34958_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_34958_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Ternary complex of CnCas12f1-sgRNA-DNA
Entire | Name: Ternary complex of CnCas12f1-sgRNA-DNA |
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Components |
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-Supramolecule #1: Ternary complex of CnCas12f1-sgRNA-DNA
Supramolecule | Name: Ternary complex of CnCas12f1-sgRNA-DNA / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Transposase
Macromolecule | Name: Transposase / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 58.60968 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MITVRKIKLT IMGDKDTRNS QYKWIRDEQY NQYRALNMGM TYLAVNDILY MNESGLEIRT IKDLKDCEKD IDKNKKEIEK LTARLEKEQ NKKNSSSEKL DEIKYKISLV ENKIEDYKLK IVELNKILEE TQKERMDIQK EFKEKYVDDL YQVLDKIPFK H LDNKSLVT ...String: MITVRKIKLT IMGDKDTRNS QYKWIRDEQY NQYRALNMGM TYLAVNDILY MNESGLEIRT IKDLKDCEKD IDKNKKEIEK LTARLEKEQ NKKNSSSEKL DEIKYKISLV ENKIEDYKLK IVELNKILEE TQKERMDIQK EFKEKYVDDL YQVLDKIPFK H LDNKSLVT QRIKADIKSD KSNGLLKGER SIRNYKRNFP LMTRGRDLKF KYDDNDDIEI KWMEGIKFKV ILGNRIKNSL EL RHTLHKV IEGKYKICDS SLQFDKNNNL ILNLTLDIPI DIVNKKVSGR VVGVDLGLKI PAYCALNDVE YIKKSIGRID DFL KVRTQM QSRRRRLQIA IQSAKGGKGR VNKLQALERF AEKEKNFAKT YNHFLSSNIV KFAVSNQAEQ INMELLSLKE TQNK SILRN WSYYQLQTMI EYKAQREGIK VKYIDPYHTS QTCSKCGNYE EGQRESQADF ICKKCGYKVN ADYNAARNIA MSNKY ITKK EESKYYKIKE SMV UniProtKB: Transposase |
-Macromolecule #2: sgRNA
Macromolecule | Name: sgRNA / type: rna / ID: 2 / Number of copies: 1 |
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Source (natural) | Organism: synthetic construct (others) |
Molecular weight | Theoretical: 75.498406 KDa |
Sequence | String: AACAUAGUUA AACUAAUAAA AACAGGGCGA UUUAACGUCC UAAGGCUGAG AGAAGUUUUU UCUACUCGGC AAGGGUUAAU CUCGAUUGU UGUGUUACCG AUCGAGCGUU UCACAAAAUG CGAGAGAAAU CUCGCAUUUU UAAUUUUGCA GUAAGGCUAG U UUUUAUAU ...String: AACAUAGUUA AACUAAUAAA AACAGGGCGA UUUAACGUCC UAAGGCUGAG AGAAGUUUUU UCUACUCGGC AAGGGUUAAU CUCGAUUGU UGUGUUACCG AUCGAGCGUU UCACAAAAUG CGAGAGAAAU CUCGCAUUUU UAAUUUUGCA GUAAGGCUAG U UUUUAUAU AAAUAUGCUA UAACUAGGGU UUUAGUUUAA CUAUGUGAAA UGUAAAUAAU AGAUGUGAAG UCGCUUU |
-Macromolecule #3: DNA (28-MER)
Macromolecule | Name: DNA (28-MER) / type: dna / ID: 3 / Number of copies: 1 / Classification: DNA |
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Source (natural) | Organism: synthetic construct (others) |
Molecular weight | Theoretical: 8.612591 KDa |
Sequence | String: (DG)(DA)(DA)(DA)(DA)(DG)(DC)(DG)(DA)(DC) (DT)(DT)(DC)(DA)(DC)(DA)(DT)(DC)(DT)(DA) (DT)(DT)(DA)(DG)(DG)(DT)(DT)(DA) |
-Macromolecule #4: DNA (5'-D(*TP*AP*AP*CP*CP*TP*AP*AP*TP*AP*GP*AP*TP*GP*TP*GP*AP*A)-3')
Macromolecule | Name: DNA (5'-D(*TP*AP*AP*CP*CP*TP*AP*AP*TP*AP*GP*AP*TP*GP*TP*GP*AP*A)-3') type: dna / ID: 4 / Number of copies: 1 / Classification: DNA |
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Source (natural) | Organism: synthetic construct (others) |
Molecular weight | Theoretical: 5.547643 KDa |
Sequence | String: (DT)(DA)(DA)(DC)(DC)(DT)(DA)(DA)(DT)(DA) (DG)(DA)(DT)(DG)(DT)(DG)(DA)(DA) |
-Experimental details
-Structure determination
![]() | single particle reconstruction |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |