+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | rT66 Alt A-form (scaffold xover asymmetry) | |||||||||
![]() | rT66 Alt A-form (scaffold xover asymmetry) | |||||||||
![]() |
| |||||||||
![]() | Origami / scaffold / tetrahedron / RNA | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 20.0 Å | |||||||||
![]() | Zhang K / Li S | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: 3D RNA-scaffolded wireframe origami Authors: Parsons M / Allan M / Li S / Shepherd T / Ratanalert S / Zhang K / Pullen K / Chiu W / Rouskin S / Bathe M | |||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 7.6 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 11.3 KB 11.3 KB | Display Display | ![]() |
Images | ![]() | 59.9 KB | ||
Others | ![]() ![]() | 7.6 MB 7.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 494.2 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 493.7 KB | Display | |
Data in XML | ![]() | 9 KB | Display | |
Data in CIF | ![]() | 10.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | rT66 Alt A-form (scaffold xover asymmetry) | ||||||||||||||||||||
Voxel size | X=Y=Z: 3.52 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Half map: #1
File | emd_34050_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #2
File | emd_34050_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : rT66 Alt A-form (scaffold xover asymmetry)
Entire | Name: rT66 Alt A-form (scaffold xover asymmetry) |
---|---|
Components |
|
-Supramolecule #1: rT66 Alt A-form (scaffold xover asymmetry)
Supramolecule | Name: rT66 Alt A-form (scaffold xover asymmetry) / type: complex / ID: 1 / Parent: 0 |
---|---|
Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.5 |
---|---|
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy
Microscope | FEI TECNAI ARCTICA |
---|---|
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 63.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.5 µm |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
-
Image processing
Startup model | Type of model: OTHER |
---|---|
Final reconstruction | Applied symmetry - Point group: T (tetrahedral) / Resolution.type: BY AUTHOR / Resolution: 20.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: EMAN2 (ver. 2.3) / Number images used: 819 |
Initial angle assignment | Type: PROJECTION MATCHING |
Final angle assignment | Type: PROJECTION MATCHING |