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- EMDB-33572: Complex of integrin alphaV/beta8 and L-TGF-beta1 at a ratio of 1:2 -
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Open data
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Basic information
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Title | Complex of integrin alphaV/beta8 and L-TGF-beta1 at a ratio of 1:2 | |||||||||
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Function / homology | ![]() ganglioside metabolic process / adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains / regulation of interleukin-23 production / branch elongation involved in mammary gland duct branching / positive regulation of primary miRNA processing / regulation of branching involved in mammary gland duct morphogenesis / positive regulation of microglia differentiation / Influenza Virus Induced Apoptosis / frontal suture morphogenesis / negative regulation of skeletal muscle tissue development ...ganglioside metabolic process / adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains / regulation of interleukin-23 production / branch elongation involved in mammary gland duct branching / positive regulation of primary miRNA processing / regulation of branching involved in mammary gland duct morphogenesis / positive regulation of microglia differentiation / Influenza Virus Induced Apoptosis / frontal suture morphogenesis / negative regulation of skeletal muscle tissue development / regulation of enamel mineralization / regulatory T cell differentiation / regulation of cartilage development / TGFBR2 MSI Frameshift Mutants in Cancer / regulation of blood vessel remodeling / tolerance induction to self antigen / regulation of striated muscle tissue development / negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target / regulation of protein import into nucleus / embryonic liver development / columnar/cuboidal epithelial cell maturation / type III transforming growth factor beta receptor binding / positive regulation of odontogenesis / Langerhans cell differentiation / negative regulation of hyaluronan biosynthetic process / positive regulation of cardiac muscle cell differentiation / myofibroblast differentiation / positive regulation of receptor signaling pathway via STAT / connective tissue replacement involved in inflammatory response wound healing / positive regulation of exit from mitosis / extracellular matrix assembly / integrin alphav-beta6 complex / integrin alphav-beta8 complex / hard palate development / transforming growth factor beta production / negative regulation of macrophage cytokine production / negative regulation of entry of bacterium into host cell / integrin alphav-beta5 complex / odontoblast differentiation / opsonin binding / TGFBR2 Kinase Domain Mutants in Cancer / positive regulation of smooth muscle cell differentiation / positive regulation of isotype switching to IgA isotypes / positive regulation of mesenchymal stem cell proliferation / integrin alphav-beta1 complex / mammary gland branching involved in thelarche / SMAD2/3 Phosphorylation Motif Mutants in Cancer / TGFBR1 KD Mutants in Cancer / Cross-presentation of particulate exogenous antigens (phagosomes) / retina vasculature development in camera-type eye / heart valve morphogenesis / extracellular matrix protein binding / macrophage derived foam cell differentiation / membrane protein intracellular domain proteolysis / response to laminar fluid shear stress / positive regulation of vasculature development / bronchiole development / hyaluronan catabolic process / ATP biosynthetic process / Laminin interactions / positive regulation of branching involved in ureteric bud morphogenesis / positive regulation of extracellular matrix assembly / receptor catabolic process / placenta blood vessel development / lens fiber cell differentiation / negative regulation of extracellular matrix disassembly / integrin alphav-beta3 complex / negative regulation of lipoprotein metabolic process / type II transforming growth factor beta receptor binding / oligodendrocyte development / TGFBR1 LBD Mutants in Cancer / alphav-beta3 integrin-PKCalpha complex / entry into host cell by a symbiont-containing vacuole / response to salt / germ cell migration / negative regulation of biomineral tissue development / positive regulation of mononuclear cell migration / alphav-beta3 integrin-HMGB1 complex / endoderm development / type I transforming growth factor beta receptor binding / phospholipid homeostasis / positive regulation of chemotaxis / negative regulation of lipid transport / negative regulation of myoblast differentiation / positive regulation of endothelial cell apoptotic process / positive regulation of vascular permeability / negative regulation of low-density lipoprotein receptor activity / cell-cell junction organization / regulation of phagocytosis / response to vitamin D / Elastic fibre formation / positive regulation of regulatory T cell differentiation / alphav-beta3 integrin-IGF-1-IGF1R complex / transforming growth factor beta binding / response to cholesterol / digestive tract development / negative regulation of interleukin-17 production / surfactant homeostasis / deubiquitinase activator activity / negative regulation of release of sequestered calcium ion into cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.24 Å | |||||||||
![]() | Duan Z / Zhang Z | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Specificity of TGF-β1 signal designated by LRRC33 and integrin αβ. Authors: Zelin Duan / Xuezhen Lin / Lixia Wang / Qiuxin Zhen / Yuefeng Jiang / Chuxin Chen / Jing Yang / Chia-Hsueh Lee / Yan Qin / Ying Li / Bo Zhao / Jianchuan Wang / Zhe Zhang / ![]() ![]() Abstract: Myeloid lineage cells present the latent form of transforming growth factor-β1 (L-TGF-β1) to the membrane using an anchor protein LRRC33. Integrin αβ activates extracellular L-TGF-β1 to trigger ...Myeloid lineage cells present the latent form of transforming growth factor-β1 (L-TGF-β1) to the membrane using an anchor protein LRRC33. Integrin αβ activates extracellular L-TGF-β1 to trigger the downstream signaling functions. However, the mechanism designating the specificity of TGF-β1 presentation and activation remains incompletely understood. Here, we report cryo-EM structures of human L-TGF-β1/LRRC33 and integrin αβ/L-TGF-β1 complexes. Combined with biochemical and cell-based analyses, we demonstrate that LRRC33 only presents L-TGF-β1 but not the -β2 or -β3 isoforms due to difference of key residues on the growth factor domains. Moreover, we reveal a 2:2 binding mode of integrin αβ and L-TGF-β1, which shows higher avidity and more efficient L-TGF-β1 activation than previously reported 1:2 binding mode. We also uncover that the disulfide-linked loop of the integrin subunit β determines its exquisite affinity to L-TGF-β1. Together, our findings provide important insights into the specificity of TGF-β1 signaling achieved by LRRC33 and integrin αβ. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 204.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 18.5 KB 18.5 KB | Display Display | ![]() |
Images | ![]() | 107.2 KB | ||
Others | ![]() ![]() | 200.2 MB 200.2 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7y1tMC ![]() 7y1rC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.055 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_33572_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_33572_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Complex of integrin alphaV/beta8 and L-TGF-beta1 at a ratio of 1:2
Entire | Name: Complex of integrin alphaV/beta8 and L-TGF-beta1 at a ratio of 1:2 |
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Components |
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-Supramolecule #1: Complex of integrin alphaV/beta8 and L-TGF-beta1 at a ratio of 1:2
Supramolecule | Name: Complex of integrin alphaV/beta8 and L-TGF-beta1 at a ratio of 1:2 type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() |
Molecular weight | Theoretical: 210 KDa |
-Macromolecule #1: Integrin alpha-V
Macromolecule | Name: Integrin alpha-V / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 69.812711 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MAFPPRRRLR LGPRGLPLLL SGLLLPLCRA FNLDVDSPAE YSGPEGSYFG FAVDFFVPSA SSRMFLLVGA PKANTTQPGI VEGGQVLKC DWSSTRRCQP IEFDATGNRD YAKDDPLEFK SHQWFGASVR SKQDKILACA PLYHWRTEMK QEREPVGTCF L QDGTKTVE ...String: MAFPPRRRLR LGPRGLPLLL SGLLLPLCRA FNLDVDSPAE YSGPEGSYFG FAVDFFVPSA SSRMFLLVGA PKANTTQPGI VEGGQVLKC DWSSTRRCQP IEFDATGNRD YAKDDPLEFK SHQWFGASVR SKQDKILACA PLYHWRTEMK QEREPVGTCF L QDGTKTVE YAPCRSQDID ADGQGFCQGG FSIDFTKADR VLLGGPGSFY WQGQLISDQV AEIVSKYDPN VYSIKYNNQL AT RTAQAIF DDSYLGYSVA VGDFNGDGID DFVSGVPRAA RTLGMVYIYD GKNMSSLYNF TGEQMAAYFG FSVAATDING DDY ADVFIG APLFMDRGSD GKLQEVGQVS VSLQRASGDF QTTKLNGFEV FARFGSAIAP LGDLDQDGFN DIAIAAPYGG EDKK GIVYI FNGRSTGLNA VPSQILEGQW AARSGCPPSF GYSMKGATDI DKNGYPDLIV GAFGVDRAIL YRARPVITVN AGLEV YPSI LNQDNKTCSL PGTALKVSCF NVRFCLKADG KGVLPRKLNF QVELLLDKLK QKGAIRRALF LYSRSPSHSK NMTISR GGL MQCEELIAYL RDESEFRDKL TPITIFMEYR LDYRTAADTT GLQPILNQFT PANISRQAHI LLDTGGLEVL FQ |
-Macromolecule #2: Integrin beta-8
Macromolecule | Name: Integrin beta-8 / type: protein_or_peptide / ID: 2 Details: The first 21 residues (MDMRVPAQLLGLLLLWFSGVL) are signal peptides. Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 53.017355 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MDMRVPAQLL GLLLLWFSGV LEDNRCASSN AASCARCLAL GPECGWCVQE DFISGGSRSE RCDIVSNLIS KGCSVDSIEY PSVHVIIPT ENEINTQVTP GEVSIQLRPG AEANFMLKVH PLKKYPVDLY YLVDVSASMH NNIEKLNSVG NDLSRKMAFF S RDFRLGFG ...String: MDMRVPAQLL GLLLLWFSGV LEDNRCASSN AASCARCLAL GPECGWCVQE DFISGGSRSE RCDIVSNLIS KGCSVDSIEY PSVHVIIPT ENEINTQVTP GEVSIQLRPG AEANFMLKVH PLKKYPVDLY YLVDVSASMH NNIEKLNSVG NDLSRKMAFF S RDFRLGFG SYVDKTVSPY ISIHPERIHN QCSDYNLDCM PPHGYIHVLS LTENITEFEK AVHRQKISGN IDTPEGGFDA ML QAAVCES HIGWRKEAKR LLLVMTDQTS HLALDSKLAG IVCPNDGNCH LKNNVYVKST TMEHPSLGQL SEKLIDNNIN VIF AVQGKQ FHWYKDLLPL LPGTIAGEIE SKAANLNNLV VEAYQKLISE VKVQVENQVQ GIYFNITAIC PDGSRKPGME GCRN VTSND EVLFNVTVTM KKCDVTGGKN YAIIKPIGFN ETAKIHIHRN CSCQCEDNRG PKGKCVDETF LDSKCFQCDE NK |
-Macromolecule #3: Transforming growth factor beta-1 proprotein
Macromolecule | Name: Transforming growth factor beta-1 proprotein / type: protein_or_peptide / ID: 3 Details: The first 16 residues (MPLLLLLPLLWAGALA) are signal peptides. Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 43.010402 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MPLLLLLPLL WAGALALSTC KTIDMELVKR KRIEAIRGQI LSKLRLASPP SQGEVPPGPL PEAVLALYNS TRDRVAGESA EPEPEPEAD YYAKEVTRVL MVETHNEIYD KFKQSTHSIY MFFNTSELRE AVPEPVLLSR AELRLLRLKL KVEQHVELYQ K YSNNSWRY ...String: MPLLLLLPLL WAGALALSTC KTIDMELVKR KRIEAIRGQI LSKLRLASPP SQGEVPPGPL PEAVLALYNS TRDRVAGESA EPEPEPEAD YYAKEVTRVL MVETHNEIYD KFKQSTHSIY MFFNTSELRE AVPEPVLLSR AELRLLRLKL KVEQHVELYQ K YSNNSWRY LSNRLLAPSD SPEWLSFDVT GVVRQWLSRG GEIEGFRLSA HCSCDSRDNT LQVDINGFTT GRRGDLATIH GM NRPFLLL MATPLERAQH LQSSRHRRAL DTNYCFSSTE KNCCVRQLYI DFRKDLGWKW IHEPKGYHAN FCLGPCPYIW SLD TQYSKV LALYNQHNPG ASAAPCCVPQ ALEPLPIVYY VGRKPKVEQL SNMIVRSCKC S |
-Macromolecule #7: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 7 / Number of copies: 5 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Macromolecule #8: CALCIUM ION
Macromolecule | Name: CALCIUM ION / type: ligand / ID: 8 / Number of copies: 5 / Formula: CA |
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Molecular weight | Theoretical: 40.078 Da |
-Macromolecule #9: MANGANESE (II) ION
Macromolecule | Name: MANGANESE (II) ION / type: ligand / ID: 9 / Number of copies: 1 / Formula: MN |
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Molecular weight | Theoretical: 54.938 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 3 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.2 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.24 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 305011 |
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Initial angle assignment | Type: NOT APPLICABLE |
Final angle assignment | Type: ANGULAR RECONSTITUTION |
-Atomic model buiding 1
Refinement | Protocol: OTHER |
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Output model | ![]() PDB-7y1t: |