[English] 日本語
![](img/lk-miru.gif)
- EMDB-33496: Tomographic reconstruction of FIB-milled cryo-lamella of HepG2 ce... -
+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Tomographic reconstruction of FIB-milled cryo-lamella of HepG2 cells containing a LD-mitochondria contact sites. | |||||||||
![]() | ||||||||||
![]() |
| |||||||||
Biological species | ![]() | |||||||||
Method | electron tomography / cryo EM | |||||||||
![]() | Xiao K / Li W / Li Z / Guo Q / Tao X / Ji W | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: The functional universe of membrane contact sites. Authors: Prinz WA / Toulmay A / Balla T | |||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 628.8 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 7.3 KB 7.3 KB | Display Display | ![]() |
Images | ![]() | 261.7 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 400.5 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 400 KB | Display | |
Data in XML | ![]() | 5.3 KB | Display | |
Data in CIF | ![]() | 5.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 21.68 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-
Sample components
-Entire : HepG2 cells with fluorescent reporter of LD-mitochondria contact
Entire | Name: HepG2 cells with fluorescent reporter of LD-mitochondria contact |
---|---|
Components |
|
-Supramolecule #1: HepG2 cells with fluorescent reporter of LD-mitochondria contact
Supramolecule | Name: HepG2 cells with fluorescent reporter of LD-mitochondria contact type: cell / ID: 1 / Parent: 0 |
---|---|
Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | electron tomography |
Aggregation state | cell |
-
Sample preparation
Buffer | pH: 7.4 |
---|---|
Vitrification | Cryogen name: ETHANE / Chamber humidity: 85 % / Chamber temperature: 310 K |
Sectioning | Focused ion beam - Instrument: OTHER / Focused ion beam - Ion: OTHER / Focused ion beam - Voltage: 30 kV / Focused ion beam - Current: 0.5 nA / Focused ion beam - Duration: 300 sec. / Focused ion beam - Temperature: 303 K / Focused ion beam - Initial thickness: 1000 nm / Focused ion beam - Final thickness: 150 nm Focused ion beam - Details: The value given for _em_focused_ion_beam.instrument is Tescan S8000G. This is not in a list of allowed values {'DB235', 'OTHER'} so OTHER is written into the XML file. |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 3.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 6.0 µm / Nominal defocus min: 5.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
-
Image processing
Final reconstruction | Algorithm: BACK PROJECTION / Number images used: 35 |
---|