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- EMDB-33417: Microtubule triplets with complete C tubule of human centrioles f... -
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Open data
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Basic information
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Title | Microtubule triplets with complete C tubule of human centrioles from HeLa cells. | |||||||||
![]() | Postprocessed map of microtubule triplets with complete C tubule of human centrioles from HeLa cells. | |||||||||
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![]() | Complex / Centriole / Microtubule / in situ / CELL CYCLE | |||||||||
Biological species | ![]() | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 31.0 Å | |||||||||
![]() | Li SG / Wang ZY / Zhu Y / Ji G / Sun F | |||||||||
Funding support | ![]()
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![]() | ![]() Title: ELI trifocal microscope: a precise system to prepare target cryo-lamellae for in situ cryo-ET study Authors: Li SG / Wang ZY / Jia X / Niu TX / Zhang JG / Yin GL / Zhang XY / Zhu Y / Ji G / Sun F | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 985.6 KB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.4 KB 12.4 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 4.7 KB | Display | ![]() |
Images | ![]() | 24.4 KB | ||
Masks | ![]() | 8 MB | ![]() | |
Filedesc metadata | ![]() | 3.8 KB | ||
Others | ![]() ![]() | 6 MB 6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 737 KB | Display | ![]() |
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Full document | ![]() | 736.6 KB | Display | |
Data in XML | ![]() | 10.5 KB | Display | |
Data in CIF | ![]() | 14 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Postprocessed map of microtubule triplets with complete C tubule of human centrioles from HeLa cells. | ||||||||||||||||||||
Voxel size | X=Y=Z: 8.6 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: Half map of microtubule triplets with complete C...
File | emd_33417_half_map_1.map | ||||||||||||
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Annotation | Half map of microtubule triplets with complete C tubule of human centrioles from HeLa cells. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map of microtubule triplets with complete C...
File | emd_33417_half_map_2.map | ||||||||||||
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Annotation | Half map of microtubule triplets with complete C tubule of human centrioles from HeLa cells. | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Microtubule triplets with complete C tubule of human centrioles f...
Entire | Name: Microtubule triplets with complete C tubule of human centrioles from HeLa cells. |
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Components |
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-Supramolecule #1: Microtubule triplets with complete C tubule of human centrioles f...
Supramolecule | Name: Microtubule triplets with complete C tubule of human centrioles from HeLa cells. type: organelle_or_cellular_component / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | cell |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 3.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 0.9 µm / Nominal defocus min: 0.5 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |