+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-32990 | |||||||||
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Title | Cryo-EM structure of RNC-RAC complex (State C3) | |||||||||
Map data | ||||||||||
Sample |
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Biological species | Saccharomyces cerevisiae (brewer's yeast) | |||||||||
Method | single particle reconstruction / cryo EM / negative staining / Resolution: 3.3 Å | |||||||||
Authors | Chen Y / Gao N | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: Structural remodeling of the ribosome associated Hsp40-Hsp70 chaperones during co-translational folding Authors: Chen Y / Tsai B / Li N / Gao N | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_32990.map.gz | 258.2 MB | EMDB map data format | |
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Header (meta data) | emd-32990-v30.xml emd-32990.xml | 10.8 KB 10.8 KB | Display Display | EMDB header |
Images | emd_32990.png | 72.2 KB | ||
Others | emd_32990_half_map_1.map.gz emd_32990_half_map_2.map.gz | 259.9 MB 258.9 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-32990 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-32990 | HTTPS FTP |
-Validation report
Summary document | emd_32990_validation.pdf.gz | 963.8 KB | Display | EMDB validaton report |
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Full document | emd_32990_full_validation.pdf.gz | 963.4 KB | Display | |
Data in XML | emd_32990_validation.xml.gz | 17 KB | Display | |
Data in CIF | emd_32990_validation.cif.gz | 20.2 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-32990 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-32990 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_32990.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.07 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_32990_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_32990_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : RNC-RAC complex (State C3)
Entire | Name: RNC-RAC complex (State C3) |
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Components |
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-Supramolecule #1: RNC-RAC complex (State C3)
Supramolecule | Name: RNC-RAC complex (State C3) / type: complex / ID: 1 / Chimera: Yes / Parent: 0 |
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Source (natural) | Organism: Saccharomyces cerevisiae (brewer's yeast) |
-Experimental details
-Structure determination
Method | negative staining, cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Staining | Type: NEGATIVE / Material: Uranyl Acetate |
Vitrification | Cryogen name: NITROGEN |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 47.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: DARK FIELD / Nominal defocus max: 1.6 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 11831 |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |