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- EMDB-32924: Cryo-EM structure of human ABCD1 E630Q in the presence of C26:0-C... -

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Basic information

Entry
Database: EMDB / ID: EMD-32924
TitleCryo-EM structure of human ABCD1 E630Q in the presence of C26:0-CoA and ATP
Map data
Sample
  • Complex: Cryo-EM structure of human ABCD1 E630Q in the presence of C26:0-CoA and ATP
    • Protein or peptide: ATP-binding cassette sub-family D member 1
  • Ligand: OCTANOYL-COENZYME A
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
KeywordsMembrane protein / ligand / complex / STRUCTURAL PROTEIN
Function / homology
Function and homology information


ABC-type fatty-acyl-CoA transporter activity / peroxisomal membrane transport / very long-chain fatty-acyl-CoA catabolic process / very long-chain fatty acyl-CoA hydrolase activity / positive regulation of unsaturated fatty acid biosynthetic process / Linoleic acid (LA) metabolism / Defective ABCD1 causes ALD / alpha-linolenic acid metabolic process / long-chain fatty acid catabolic process / long-chain fatty acid import into peroxisome ...ABC-type fatty-acyl-CoA transporter activity / peroxisomal membrane transport / very long-chain fatty-acyl-CoA catabolic process / very long-chain fatty acyl-CoA hydrolase activity / positive regulation of unsaturated fatty acid biosynthetic process / Linoleic acid (LA) metabolism / Defective ABCD1 causes ALD / alpha-linolenic acid metabolic process / long-chain fatty acid catabolic process / long-chain fatty acid import into peroxisome / very long-chain fatty acid catabolic process / alpha-linolenic acid (ALA) metabolism / regulation of fatty acid beta-oxidation / Beta-oxidation of very long chain fatty acids / sterol homeostasis / Class I peroxisomal membrane protein import / very long-chain fatty acid metabolic process / peroxisome organization / regulation of mitochondrial depolarization / fatty acyl-CoA hydrolase activity / ABC transporters in lipid homeostasis / myelin maintenance / regulation of cellular response to oxidative stress / Hydrolases; Acting on ester bonds; Thioester hydrolases / linoleic acid metabolic process / positive regulation of fatty acid beta-oxidation / regulation of oxidative phosphorylation / fatty acid elongation / Translocases; Catalysing the translocation of other compounds; Linked to the hydrolysis of a nucleoside triphosphate / peroxisomal membrane / long-chain fatty acid transmembrane transporter activity / fatty acid beta-oxidation / ATPase-coupled transmembrane transporter activity / negative regulation of cytokine production involved in inflammatory response / fatty acid homeostasis / negative regulation of reactive oxygen species biosynthetic process / neuron projection maintenance / mitochondrial membrane / ADP binding / peroxisome / protein heterodimerization activity / lysosomal membrane / endoplasmic reticulum membrane / perinuclear region of cytoplasm / enzyme binding / protein homodimerization activity / ATP hydrolysis activity / ATP binding / identical protein binding / membrane / cytosol / cytoplasm
Similarity search - Function
Peroxysomal long chain fatty acyl transporter / ABC transporter transmembrane region 2 / : / ABC transporter type 1, transmembrane domain / ABC transporter integral membrane type-1 fused domain profile. / ABC transporter type 1, transmembrane domain superfamily / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain ...Peroxysomal long chain fatty acyl transporter / ABC transporter transmembrane region 2 / : / ABC transporter type 1, transmembrane domain / ABC transporter integral membrane type-1 fused domain profile. / ABC transporter type 1, transmembrane domain superfamily / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
ATP-binding cassette sub-family D member 1
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.3 Å
AuthorsChao X / Li-Na J / Lin T
Funding support China, 3 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)31770897 China
National Natural Science Foundation of China (NSFC)81801294 China
Other private2017YFA0504300 China
CitationJournal: Signal Transduct Target Ther / Year: 2023
Title: Structural insights into substrate recognition and translocation of human peroxisomal ABC transporter ALDP.
Authors: Chao Xiong / Li-Na Jia / Wei-Xi Xiong / Xin-Tong Wu / Liu-Lin Xiong / Ting-Hua Wang / Dong Zhou / Zhen Hong / Zheng Liu / Lin Tang /
Abstract: Dysfunctions of ATP-binding cassette, subfamily D, member 1 (ABCD1) cause X-linked adrenoleukodystrophy, a rare neurodegenerative disease that affects all human tissues. Residing in the peroxisome ...Dysfunctions of ATP-binding cassette, subfamily D, member 1 (ABCD1) cause X-linked adrenoleukodystrophy, a rare neurodegenerative disease that affects all human tissues. Residing in the peroxisome membrane, ABCD1 plays a role in the translocation of very long-chain fatty acids for their β-oxidation. Here, the six cryo-electron microscopy structures of ABCD1 in four distinct conformational states were presented. In the transporter dimer, two transmembrane domains form the substrate translocation pathway, and two nucleotide-binding domains form the ATP-binding site that binds and hydrolyzes ATP. The ABCD1 structures provide a starting point for elucidating the substrate recognition and translocation mechanism of ABCD1. Each of the four inward-facing structures of ABCD1 has a vestibule that opens to the cytosol with variable sizes. Hexacosanoic acid (C26:0)-CoA substrate binds to the transmembrane domains (TMDs) and stimulates the ATPase activity of the nucleotide-binding domains (NBDs). W339 from the transmembrane helix 5 (TM5) is essential for binding substrate and stimulating ATP hydrolysis by substrate. ABCD1 has a unique C-terminal coiled-coil domain that negatively modulates the ATPase activity of the NBDs. Furthermore, the structure of ABCD1 in the outward-facing state indicates that ATP molecules pull the two NBDs together and open the TMDs to the peroxisomal lumen for substrate release. The five structures provide a view of the substrate transport cycle and mechanistic implication for disease-causing mutations.
History
DepositionFeb 22, 2022-
Header (metadata) releaseMar 22, 2023-
Map releaseMar 22, 2023-
UpdateJun 26, 2024-
Current statusJun 26, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_32924.png

Downloads & links

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Map

FileDownload / File: emd_32924.map.gz / Format: CCP4 / Size: 209.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.83 Å/pix.
x 380 pix.
= 315.4 Å		0.83 Å/pix.
x 380 pix.
= 315.4 Å		0.83 Å/pix.
x 380 pix.
= 315.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.83 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.4
Minimum - Maximum-1.4466268 - 2.0531492
Average (Standard dev.)-0.0008579075 (±0.029752463)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions380380380
Spacing380380380
CellA=B=C: 315.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Cryo-EM structure of human ABCD1 E630Q in the presence of C26:0-C...

EntireName: Cryo-EM structure of human ABCD1 E630Q in the presence of C26:0-CoA and ATP
Components
  • Complex: Cryo-EM structure of human ABCD1 E630Q in the presence of C26:0-CoA and ATP
    • Protein or peptide: ATP-binding cassette sub-family D member 1
  • Ligand: OCTANOYL-COENZYME A
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE

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Supramolecule #1: Cryo-EM structure of human ABCD1 E630Q in the presence of C26:0-C...

SupramoleculeName: Cryo-EM structure of human ABCD1 E630Q in the presence of C26:0-CoA and ATP
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: ATP-binding cassette sub-family D member 1

MacromoleculeName: ATP-binding cassette sub-family D member 1 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
EC number: Hydrolases; Acting on ester bonds; Thioester hydrolases
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 85.194125 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MDYKDDDDKG SENLYFQSMP VLSRPRPWRG NTLKRTAVLL ALAAYGAHKV YPLVRQCLAP ARGLQAPAGE PTQEASGVAA AKAGMNRVF LQRLLWLLRL LFPRVLCRET GLLALHSAAL VSRTFLSVYV ARLDGRLARC IVRKDPRAFG WQLLQWLLIA L PATFVNSA ...String:
MDYKDDDDKG SENLYFQSMP VLSRPRPWRG NTLKRTAVLL ALAAYGAHKV YPLVRQCLAP ARGLQAPAGE PTQEASGVAA AKAGMNRVF LQRLLWLLRL LFPRVLCRET GLLALHSAAL VSRTFLSVYV ARLDGRLARC IVRKDPRAFG WQLLQWLLIA L PATFVNSA IRYLEGQLAL SFRSRLVAHA YRLYFSQQTY YRVSNMDGRL RNPDQSLTED VVAFAASVAH LYSNLTKPLL DV AVTSYTL LRAARSRGAG TAWPSAIAGL VVFLTANVLR AFSPKFGELV AEEARRKGEL RYMHSRVVAN SEEIAFYGGH EVE LALLQR SYQDLASQIN LILLERLWYV MLEQFLMKYV WSASGLLMVA VPIITATGYS ESDAEAVKKA ALEKKEEELV SERT EAFTI ARNLLTAAAD AIERIMSSYK EVTELAGYTA RVHEMFQVFE DVQRCHFKRP RELEDAQAGS GTIGRSGVRV EGPLK IRGQ VVDVEQGIIC ENIPIVTPSG EVVVASLNIR VEEGMHLLIT GPNGCGKSSL FRILGGLWPT YGGVLYKPPP QRMFYI PQR PYMSVGSLRD QVIYPDSVED MQRKGYSEQD LEAILDVVHL HHILQREGGW EAMCDWKDVL SGGEKQRIGM ARMFYHR PK YALLDQCTSA VSIDVEGKIF QAAKDAGIAL LSITHRPSLW KYHTHLLQFD GEGGWKFEKL DSAARLSLTE EKQRLEQQ L AGIPKMQRRL QELCQILGEA VAPAHVPAPS PQGPGGLQGA ST

UniProtKB: ATP-binding cassette sub-family D member 1

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Macromolecule #2: OCTANOYL-COENZYME A

MacromoleculeName: OCTANOYL-COENZYME A / type: ligand / ID: 2 / Number of copies: 1 / Formula: CO8
Molecular weightTheoretical: 893.73 Da
Chemical component information

ChemComp-CO8:
OCTANOYL-COENZYME A

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Macromolecule #3: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 3 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation state3D array

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI EAGLE (4k x 4k) / Average electron dose: 66.5 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 3010722
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: COMMON LINE

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