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- EMDB-3286: Evidence for a conformational switch in Influenzavirus M1 and its... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-3286 | |||||||||
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Title | Evidence for a conformational switch in Influenzavirus M1 and its role in filamentous virion architecture | |||||||||
![]() | Reconstruction of inner membrane leaflet, M1 and attached densities at leading virion tips | |||||||||
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![]() | Influenza A virus / FLUAV / polymerase / M1 / crown / leading tip / trailing tip / filamentous | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 76.0 Å | |||||||||
![]() | Kiss G / Abdulsattar BO / Phapugrangkul P / Birch K / Jones IM / Neuman BW | |||||||||
![]() | ![]() Title: Evidence for a conformational switch in Influenzavirus M1 and its role in filamentous virion architecture Authors: Kiss G / Abdulsattar BO / Phapugrangkul P / Birch K / Jones IM / Neuman BW | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 923.8 KB | ![]() | |
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Header (meta data) | ![]() ![]() | 8.7 KB 8.7 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 2.5 KB | Display | ![]() |
Images | ![]() | 84.9 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 225.1 KB | Display | ![]() |
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Full document | ![]() | 224.2 KB | Display | |
Data in XML | ![]() | 6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Reconstruction of inner membrane leaflet, M1 and attached densities at leading virion tips | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 10 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Leading tip of filamentous Influenza A virions including inner le...
Entire | Name: Leading tip of filamentous Influenza A virions including inner leaflet, M1 and internal crown |
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Components |
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-Supramolecule #1000: Leading tip of filamentous Influenza A virions including inner le...
Supramolecule | Name: Leading tip of filamentous Influenza A virions including inner leaflet, M1 and internal crown type: sample / ID: 1000 Details: Compare to matched image of the opposite virion tip Number unique components: 1 |
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-Supramolecule #1: Influenza A virus
Supramolecule | Name: Influenza A virus / type: virus / ID: 1 / Name.synonym: Influenza virus Details: Reconstruction of the inner layer of the viral envelope and attached densities likely representing polymerase molecules from native virions of mixed A/Aichi/X31 and A/Udorn strains. Map is ...Details: Reconstruction of the inner layer of the viral envelope and attached densities likely representing polymerase molecules from native virions of mixed A/Aichi/X31 and A/Udorn strains. Map is Gaussian filtered to 7.6 nm the 0.5 FSC. Reconstructed in EMAN2. NCBI-ID: 11320 / Sci species name: Influenza A virus / Virus type: VIRION / Virus isolate: OTHER / Virus enveloped: Yes / Virus empty: No / Syn species name: Influenza virus |
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Host (natural) | Organism: ![]() |
Host system | Organism: ![]() |
Virus shell | Shell ID: 1 / Name: M1 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | particle |
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Sample preparation
Vitrification | Cryogen name: ETHANE / Chamber humidity: 90 % / Instrument: FEI VITROBOT MARK III Details: Details for EM and reconstruction published in Calder et al., Proc Natl Acad Sci U S A. 2010 Jun 8; 107(23): 10685 to 10690. Method: Blotted 4s before plunging |
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Electron microscopy
Microscope | OTHER |
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Date | Jan 1, 2010 |
Image recording | Category: CCD / Film or detector model: FEI EAGLE (2k x 2k) / Average electron dose: 50 e/Å2 |
Electron beam | Acceleration voltage: 120 kV / Electron source: TUNGSTEN HAIRPIN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Sample stage | Specimen holder model: GATAN LIQUID NITROGEN |