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- EMDB-32096: Cryo-EM structure of the ATP-binding cassette sub-family D member... -
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Open data
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Basic information
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Title | Cryo-EM structure of the ATP-binding cassette sub-family D member 1 from Homo sapiens | |||||||||
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![]() | Membrane protein / LIPID TRANSPORT / TRANSLOCASE | |||||||||
Function / homology | ![]() ABC-type fatty-acyl-CoA transporter activity / peroxisomal membrane transport / very long-chain fatty-acyl-CoA catabolic process / very long-chain fatty acyl-CoA hydrolase activity / positive regulation of unsaturated fatty acid biosynthetic process / Linoleic acid (LA) metabolism / Defective ABCD1 causes ALD / alpha-linolenic acid metabolic process / long-chain fatty acid catabolic process / long-chain fatty acid import into peroxisome ...ABC-type fatty-acyl-CoA transporter activity / peroxisomal membrane transport / very long-chain fatty-acyl-CoA catabolic process / very long-chain fatty acyl-CoA hydrolase activity / positive regulation of unsaturated fatty acid biosynthetic process / Linoleic acid (LA) metabolism / Defective ABCD1 causes ALD / alpha-linolenic acid metabolic process / long-chain fatty acid catabolic process / long-chain fatty acid import into peroxisome / very long-chain fatty acid catabolic process / alpha-linolenic acid (ALA) metabolism / regulation of fatty acid beta-oxidation / Beta-oxidation of very long chain fatty acids / Class I peroxisomal membrane protein import / very long-chain fatty acid metabolic process / sterol homeostasis / peroxisome organization / regulation of mitochondrial depolarization / fatty acyl-CoA hydrolase activity / ABC transporters in lipid homeostasis / myelin maintenance / regulation of cellular response to oxidative stress / Hydrolases; Acting on ester bonds; Thioester hydrolases / positive regulation of fatty acid beta-oxidation / regulation of oxidative phosphorylation / fatty acid elongation / Translocases; Catalysing the translocation of other compounds; Linked to the hydrolysis of a nucleoside triphosphate / peroxisomal membrane / long-chain fatty acid transmembrane transporter activity / fatty acid beta-oxidation / ATPase-coupled transmembrane transporter activity / negative regulation of cytokine production involved in inflammatory response / fatty acid homeostasis / negative regulation of reactive oxygen species biosynthetic process / linoleic acid metabolic process / neuron projection maintenance / mitochondrial membrane / ADP binding / peroxisome / protein heterodimerization activity / lysosomal membrane / endoplasmic reticulum membrane / perinuclear region of cytoplasm / enzyme binding / protein homodimerization activity / ATP hydrolysis activity / ATP binding / identical protein binding / membrane / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.4 Å | |||||||||
![]() | Yang GH / Jia YT / Zhang YM | |||||||||
Funding support | 1 items
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![]() | ![]() Title: Structural and functional insights of the human peroxisomal ABC transporter ALDP. Authors: Yutian Jia / Yanming Zhang / Wenhao Wang / Jianlin Lei / Zhengxin Ying / Guanghui Yang / ![]() Abstract: Adrenoleukodystrophy protein (ALDP) is responsible for the transport of very-long-chain fatty acids (VLCFAs) and corresponding CoA-esters across the peroxisomal membrane. Dysfunction of ALDP leads to ...Adrenoleukodystrophy protein (ALDP) is responsible for the transport of very-long-chain fatty acids (VLCFAs) and corresponding CoA-esters across the peroxisomal membrane. Dysfunction of ALDP leads to peroxisomal metabolic disorder exemplified by X-linked adrenoleukodystrophy (ALD). Hundreds of ALD-causing mutations have been identified on ALDP. However, the pathogenic mechanisms of these mutations are restricted to clinical description due to limited structural and biochemical characterization. Here we report the cryo-electron microscopy structure of human ALDP with nominal resolution at 3.4 Å. ALDP exhibits a cytosolic-facing conformation. Compared to other lipid ATP-binding cassette transporters, ALDP has two substrate binding cavities formed by the transmembrane domains. Such structural organization may be suitable for the coordination of VLCFAs. Based on the structure, we performed integrative analysis of the cellular trafficking, protein thermostability, ATP hydrolysis, and the transport activity of representative mutations. These results provide a framework for understanding the working mechanism of ALDP and pathogenic roles of disease-associated mutations. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 28.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.4 KB 9.4 KB | Display Display | ![]() |
Images | ![]() | 54.1 KB | ||
Filedesc metadata | ![]() | 5.3 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7vr1MC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.0979 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Human ATP-binding cassette sub-family D member 1
Entire | Name: Human ATP-binding cassette sub-family D member 1 |
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Components |
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-Supramolecule #1: Human ATP-binding cassette sub-family D member 1
Supramolecule | Name: Human ATP-binding cassette sub-family D member 1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: ATP-binding cassette sub-family D member 1
Macromolecule | Name: ATP-binding cassette sub-family D member 1 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO / EC number: ec: 7.6.2.4 |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 83.040867 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MPVLSRPRPW RGNTLKRTAV LLALAAYGAH KVYPLVRQCL APARGLQAPA GEPTQEASGV AAAKAGMNRV FLQRLLWLLR LLFPRVLCR ETGLLALHSA ALVSRTFLSV YVARLDGRLA RCIVRKDPRA FGWQLLQWLL IALPATFVNS AIRYLEGQLA L SFRSRLVA ...String: MPVLSRPRPW RGNTLKRTAV LLALAAYGAH KVYPLVRQCL APARGLQAPA GEPTQEASGV AAAKAGMNRV FLQRLLWLLR LLFPRVLCR ETGLLALHSA ALVSRTFLSV YVARLDGRLA RCIVRKDPRA FGWQLLQWLL IALPATFVNS AIRYLEGQLA L SFRSRLVA HAYRLYFSQQ TYYRVSNMDG RLRNPDQSLT EDVVAFAASV AHLYSNLTKP LLDVAVTSYT LLRAARSRGA GT AWPSAIA GLVVFLTANV LRAFSPKFGE LVAEEARRKG ELRYMHSRVV ANSEEIAFYG GHEVELALLQ RSYQDLASQI NLI LLERLW YVMLEQFLMK YVWSASGLLM VAVPIITATG YSESDAEAVK KAALEKKEEE LVSERTEAFT IARNLLTAAA DAIE RIMSS YKEVTELAGY TARVHEMFQV FEDVQRCHFK RPRELEDAQA GSGTIGRSGV RVEGPLKIRG QVVDVEQGII CENIP IVTP SGEVVVASLN IRVEEGMHLL ITGPNGCGKS SLFRILGGLW PTYGGVLYKP PPQRMFYIPQ RPYMSVGSLR DQVIYP DSV EDMQRKGYSE QDLEAILDVV HLHHILQREG GWEAMCDWKD VLSGGEKQRI GMARMFYHRP KYALLDECTS AVSIDVE GK IFQAAKDAGI ALLSITHRPS LWKYHTHLLQ FDGEGGWKFE KLDSAARLSL TEEKQRLEQQ LAGIPKMQRR LQELCQIL G EAVAPAHVPA PSPQGPGGLQ GAST UniProtKB: ATP-binding cassette sub-family D member 1 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 1.5625 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.5 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 472168 |
Initial angle assignment | Type: PROJECTION MATCHING |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |