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- EMDB-32035: Structure of PA-tag modified E. hirae V-ATPase by Volta phase plate -
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Open data
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Basic information
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Title | Structure of PA-tag modified E. hirae V-ATPase by Volta phase plate | |||||||||
![]() | E. hirae V-ATPase with PA-tag | |||||||||
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Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 6.7 Å | |||||||||
![]() | Burton-Smith RN / Murata K | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Examining the structure of E. hirae V-ATPase using Volta- and Zernike-type phase plate cryo-EM Authors: Burton-Smith RN / Murata K | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 113.3 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 7.1 KB 7.1 KB | Display Display | ![]() |
Images | ![]() | 48.6 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 414.7 KB | Display | ![]() |
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Full document | ![]() | 414.2 KB | Display | |
Data in XML | ![]() | 6.5 KB | Display | |
Data in CIF | ![]() | 7.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | E. hirae V-ATPase with PA-tag | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.113 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : V-ATPase with PA-tag fused to rotor
Entire | Name: V-ATPase with PA-tag fused to rotor |
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Components |
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-Supramolecule #1: V-ATPase with PA-tag fused to rotor
Supramolecule | Name: V-ATPase with PA-tag fused to rotor / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Specialist optics | Phase plate: VOLTA PHASE PLATE |
Image recording | Film or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 54.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: OTHER / Details: Ab initio |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 6.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 46959 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |