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- EMDB-31817: Negative staining (NS)-EM structure of SARS-CoV-2 S-Beta variant ... -
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Open data
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Basic information
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Title | Negative staining (NS)-EM structure of SARS-CoV-2 S-Beta variant in complex with neutralizing antibodies, RBD-chAb-15 and RBD-chAb45 | |||||||||||||||
![]() | NSEM structure of SARS-CoV-2 S-Beta variant in complex with neutralizing antibodies, RBD-chAb-15 and RBD-chAb45 | |||||||||||||||
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Biological species | ![]() ![]() | |||||||||||||||
Method | single particle reconstruction / negative staining / Resolution: 5.1 Å | |||||||||||||||
![]() | Yu PY / Yang TJ / Chang YC / Wu HC / Hsu STD | |||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Negative staining (NS)-EM structure of SARS-CoV-2 S-Beta variant in complex with neutralizing antibodies, RBD-chAb-15 and RBD-chAb45 Authors: Yu PY / Yang TJ / Chang YC / Wu HC / Hsu STD | |||||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 31.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 10.9 KB 10.9 KB | Display Display | ![]() |
Images | ![]() | 37.5 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | NSEM structure of SARS-CoV-2 S-Beta variant in complex with neutralizing antibodies, RBD-chAb-15 and RBD-chAb45 | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.7 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : SARS-CoV-2 spike glycoprotein in complex with neutralizing antibodies
Entire | Name: SARS-CoV-2 spike glycoprotein in complex with neutralizing antibodies |
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Components |
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-Supramolecule #1: SARS-CoV-2 spike glycoprotein in complex with neutralizing antibodies
Supramolecule | Name: SARS-CoV-2 spike glycoprotein in complex with neutralizing antibodies type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 Details: S-Beta variant, two neutralizing antibodies RBD-chAb-15 and RBD-chAb-45 |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Experimental: 540 KDa |
Recombinant expression | Organism: ![]() |
-Experimental details
-Structure determination
Method | negative staining |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.05 mg/mL | ||||||||||||
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Buffer | pH: 7.6 Component:
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Staining | Type: NEGATIVE / Material: Uranyl Formate |
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Electron microscopy
Microscope | FEI TECNAI F20 |
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Image recording | Film or detector model: GATAN ULTRASCAN 4000 (4k x 4k) / Average exposure time: 1.0 sec. / Average electron dose: 30.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.0 mm |
Sample stage | Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Tecnai F20 / Image courtesy: FEI Company |