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Yorodumi- EMDB-31653: One conformation of the full length human IGF1R/IGF1 complex. Onl... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-31653 | |||||||||
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Title | One conformation of the full length human IGF1R/IGF1 complex. Only the extracellular region of the complex is resolved | |||||||||
Map data | one conformation of the IGF1R/IGF1 complex | |||||||||
Sample |
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Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.1 Å | |||||||||
Authors | Zhang X / Wei TZ / Wu C / Liu C / Zhang J | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: One conformation of the full length human IGF1R/IGF1 complex. Only the extracellular region of the complex is resolved Authors: Zhang X / Wei TZ / Wu C / Liu C / Zhang J | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_31653.map.gz | 3.8 MB | EMDB map data format | |
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Header (meta data) | emd-31653-v30.xml emd-31653.xml | 10.7 KB 10.7 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_31653_fsc.xml | 9.2 KB | Display | FSC data file |
Images | emd_31653.png | 43.8 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-31653 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-31653 | HTTPS FTP |
-Validation report
Summary document | emd_31653_validation.pdf.gz | 333.2 KB | Display | EMDB validaton report |
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Full document | emd_31653_full_validation.pdf.gz | 332.8 KB | Display | |
Data in XML | emd_31653_validation.xml.gz | 10.7 KB | Display | |
Data in CIF | emd_31653_validation.cif.gz | 14.1 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-31653 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-31653 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_31653.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | one conformation of the IGF1R/IGF1 complex | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.08 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : Complex of the dimer IGF1R with IGF1
Entire | Name: Complex of the dimer IGF1R with IGF1 |
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Components |
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-Supramolecule #1: Complex of the dimer IGF1R with IGF1
Supramolecule | Name: Complex of the dimer IGF1R with IGF1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 / Details: IGF1R-ECD/IGF1 complex |
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Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Human (human) / Recombinant cell: HEK-293F / Recombinant plasmid: pCDNA3.1 |
Molecular weight | Theoretical: 320 KDa |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 1.5 mg/mL |
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Buffer | pH: 7.4 / Component - Formula: PBS Details: PBS buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4 |
Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.038 kPa / Details: The grid was coated with glod prior to use. |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV Details: wait time 5 seconds, force 0,blot for 3 seconds before plunging. |
Details | This sample was monodisperse |
-Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average exposure time: 5.76 sec. / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |