EMDB-31600: The cryo-EM map of the core region from the X. laevis NPC CR subunit

Basic information

Entry

Database: EMDB / ID: EMD-31600

• Title: The cryo-EM map of the core region from the X. laevis NPC CR subunit
• Map data: composite map for the Xenopus laevie CR subunit.

Sample

• Complex: Xenopus laevis Nuclear Pore Complex (NPC)
  • Protein or peptide: x 16 types

Function / homology

Function:

nephron development / COPII-coated vesicle budding / nuclear pore organization / nuclear pore outer ring / nuclear pore complex assembly / COPII vesicle coat / attachment of mitotic spindle microtubules to kinetochore / structural constituent of nuclear pore / mitotic metaphase chromosome alignment / ribosomal small subunit export from nucleus / ribosomal large subunit export from nucleus / mRNA transport / intracellular transport / nuclear pore / positive regulation of TORC1 signaling / nuclear periphery / kinetochore / protein transport / nuclear membrane / lysosomal membrane / cell division / structural molecule activity / metal ion binding / nucleus / cytosol

Domain/homology:

Protein Sec13 / Nucleoporin Seh1 / Nucleoporin Nup88 / Nuclear pore component / Nucleoporin NUP88/NUP82 / Nuclear pore complex protein NUP98-NUP96 / Nucleoporin, Nup155-like, C-terminal, subdomain 3 / Nucleoporin Nup37 / Nucleoporin Nup85-like / Nup85 Nucleoporin / Nuclear pore protein 84/107 / Nuclear pore protein 84 / 107 / Nuclear pore complex protein Nup133-like / Nup358/RanBP2 E3 ligase domain / Nup358/RanBP2 E3 ligase domain / Nucleoporin, Nup155-like / Nucleoporin, Nup155-like, C-terminal, subdomain 1 / Nucleoporin, Nup155-like, C-terminal, subdomain 2 / Nucleoporin Nup186/Nup192/Nup205 / Nuclear pore complex scaffold, nucleoporins 186/192/205 / Nucleoporin interacting component Nup93/Nic96 / Nup93/Nic96 / Nucleoporin, Nup133/Nup155-like, C-terminal / Nucleoporin FG repeat / Nucleoporin FG repeat region / Non-repetitive/WGA-negative nucleoporin C-terminal / Nucleoporin, Nup133/Nup155-like, N-terminal / Nup133 N terminal like / Sec13/Seh1 family / Nuclear pore complex protein NUP96, C-terminal domain / Nuclear protein 96 / Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain / Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain superfamily / Nucleoporin autopeptidase / NUP C-terminal domain profile. / Nucleoporin peptidase S59-like / Ran binding protein RanBP1-like / Ran binding domain / RanBP1 domain / Ran binding domain type 1 profile. / Ran-binding domain / Zinc finger domain / Zn-finger in Ran binding protein and others / Zinc finger RanBP2 type profile. / Zinc finger RanBP2-type signature. / Zinc finger, RanBP2-type superfamily / Zinc finger, RanBP2-type / TPR repeat profile. / Tetratricopeptide repeats / Tetratricopeptide repeat / Tetratricopeptide-like helical domain superfamily / PH-like domain superfamily / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily

Component:

Nuclear pore complex protein Nup133 / Nuclear pore complex protein Nup98-Nup96 / RANBP2-like and GRIP domain-containing protein 3 isoform X2 / Nuclear pore complex protein / MGC154553 protein / MGC83295 protein / MGC83926 protein / Nuclear pore complex protein Nup85 / Nucleoporin SEH1-B / Protein SEC13 homolog / Nucleoporin Nup88A / Nucleoporin 155kDa L homeolog / Nuclear pore complex protein Nup93

• Biological species: Xenopus laevis (African clawed frog) / African clawed frog (African clawed frog)
• Method: single particle reconstruction / cryo EM / Resolution: 3.7 Å
• Authors: Shi Y / Huang G / Zhan X

Funding support

China, 1 items

Organization	Grant number	Country
National Natural Science Foundation of China (NSFC)		China

• Citation: Journal: Science / Year: 2022; Title: Structure of the cytoplasmic ring of the nuclear pore complex.; Authors: Xuechen Zhu / Gaoxingyu Huang / Chao Zeng / Xiechao Zhan / Ke Liang / Qikui Xu / Yanyu Zhao / Pan Wang / Qifan Wang / Qiang Zhou / Qinghua Tao / Minhao Liu / Jianlin Lei / Chuangye Yan / Yigong Shi / ; Abstract: INTRODUCTION The nuclear pore complex (NPC) resides on the nuclear envelope (NE) and mediates nucleocytoplasmic cargo transport. As one of the largest cellular machineries, a vertebrate NPC consists of cytoplasmic filaments, a cytoplasmic ring (CR), an inner ring, a nuclear ring, a nuclear basket, and a luminal ring. Each NPC has eight repeating subunits. Structure determination of NPC is a prerequisite for understanding its functional mechanism. In the past two decades, integrative modeling, which combines x-ray structures of individual nucleoporins and subcomplexes with cryo-electron tomography reconstructions, has played a crucial role in advancing our knowledge about the NPC. The CR has been a major focus of structural investigation. The CR subunit of human NPC was reconstructed by cryo-electron tomography through subtomogram averaging to an overall resolution of ~20 Å, with local resolution up to ~15 Å. Each CR subunit comprises two Y-shaped multicomponent complexes known as the inner and outer Y complexes. Eight inner and eight outer Y complexes assemble in a head-to-tail fashion to form the proximal and distal rings, respectively, constituting the CR scaffold. To achieve higher resolution of the CR, we used single-particle cryo-electron microscopy (cryo-EM) to image the intact NPC from the NE of oocytes. Reconstructions of the core region and the Nup358 region of the CR subunit had been achieved at average resolutions of 5 to 8 Å, allowing identification of secondary structural elements. RATIONALE Packing interactions among the components of the CR subunit were poorly defined by all previous EM maps. Additional components of the CR subunit are strongly suggested by the EM maps of 5- to 8-Å resolution but remain to be identified. Addressing these issues requires improved resolution of the cryo-EM reconstruction. Therefore, we may need to enhance sample preparation, optimize image acquisition, and develop an effective data-processing strategy. RESULTS To reduce conformational heterogeneity of the sample, we spread the opened NE onto the grids with minimal force and used the chemical cross-linker glutaraldehyde to stabilize the NPC. To alleviate orientation bias of the NPC, we tilted sample grids and imaged the sample with higher electron dose at higher angles. We improved the image-processing protocol. With these efforts, the average resolutions for the core and the Nup358 regions have been improved to 3.7 and 4.7 Å, respectively. The highest local resolution of the core region reaches 3.3 Å. In addition, a cryo-EM structure of the N-terminal α-helical domain of Nup358 has been resolved at 3.0-Å resolution. These EM maps allow the identification of five copies of Nup358, two copies of Nup93, two copies of Nup205, and two copies of Y complexes in each CR subunit. Relying on the EM maps and facilitated by AlphaFold prediction, we have generated a final model for the CR of the NPC. Our model of the CR subunit includes 19,037 amino acids in 30 nucleoporins. A previously unknown C-terminal fragment of Nup160 was found to constitute a key part of the vertex, in which the short arm, long arm, and stem of the Y complex meet. The Nup160 C-terminal fragment directly binds the β-propeller proteins Seh1 and Sec13. Two Nup205 molecules, which do not contact each other, bind the inner and outer Y complexes through distinct interfaces. Conformational elasticity of the two Nup205 molecules may underlie their versatility in binding to different nucleoporins in the proximal and distal CR rings. Two Nup93 molecules, each comprising an N-terminal extended helix and an ACE1 domain, bridge the Y complexes and Nup205. Nup93 and Nup205 together play a critical role in mediating the contacts between neighboring CR subunits. Five Nup358 molecules, each in the shape of a shrimp tail and named "the clamp," hold the stems of both Y complexes. The innate conformational elasticity allows each Nup358 clamp to adapt to a distinct local environment for optimal interactions with neighboring nucleoporins. In each CR subunit, the α-helical nucleoporins appear to provide the conformational elasticity; the 12 β-propellers may strengthen the scaffold. CONCLUSION Our EM map-based model of the CR subunit substantially expands the molecular mass over the reported composite models of vertebrate CR subunit. In addition to the Y complexes, five Nup358, two Nup205, and two Nup93 molecules constitute the key components of the CR. The improved EM maps reveal insights into the interfaces among the nucleoporins of the CR. [Figure: see text].

History

Deposition	Jul 31, 2021	-
Header (metadata) release	Nov 9, 2022	-
Map release	Nov 9, 2022	-
Update	Nov 9, 2022	-
Current status	Nov 9, 2022	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_31600.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: composite map for the Xenopus laevie CR subunit.
• Voxel size: X=Y=Z: 1.387 Å

Density

Contour Level	By AUTHOR: 0.35
Minimum - Maximum	-0.3342007 - 5.3342113
Average (Standard dev.)	0.0399166 (±0.08190157)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 512 512 512 Spacing 512 512 512
Cell	A=B=C: 710.144 Å α=β=γ: 90.0 °

Supplemental data

Additional map: local refined map for the core region of...

• File: emd_31600_additional_1.map
• Annotation: local refined map for the core region of the Xenopus laevis CR subunit

Sample components

Entire : Xenopus laevis Nuclear Pore Complex (NPC)

• Entire: Name: Xenopus laevis Nuclear Pore Complex (NPC)

Components

• Complex: Xenopus laevis Nuclear Pore Complex (NPC)
  • Protein or peptide: MGC83295 protein
  • Protein or peptide: Nuclear pore complex protein Nup85Nuclear pore
  • Protein or peptide: MGC154553 protein
  • Protein or peptide: Nucleoporin SEH1-B
  • Protein or peptide: outer Nup160
  • Protein or peptide: MGC83926 protein
  • Protein or peptide: Nuclear pore complex protein Nup98-Nup96Nuclear pore
  • Protein or peptide: Protein SEC13 homolog
  • Protein or peptide: Nuclear pore complex proteinNuclear pore
  • Protein or peptide: nuclear pore complex protein Nup133
  • Protein or peptide: Nup358 complex, clamps
  • Protein or peptide: Nuclear pore complex protein Nup93Nuclear pore
  • Protein or peptide: Nup155-prov protein
  • Protein or peptide: outer Nup133
  • Protein or peptide: Nucleoporin Nup88A
  • Protein or peptide: Nup98

Supramolecule #1: Xenopus laevis Nuclear Pore Complex (NPC)

• Supramolecule: Name: Xenopus laevis Nuclear Pore Complex (NPC) / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#13, #16, #15, #14
• Source (natural): Organism: Xenopus laevis (African clawed frog)

Macromolecule #1: MGC83295 protein

• Macromolecule: Name: MGC83295 protein / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 227.854141 KDa

Sequence

String:

MAAQLALNSE ASLWGPYREI WQTVLSALIK RQPEAVHSLD IVLKKYKPDF ISLFKNPPKS AQQHERVQKA STEGIPIKGT QRTRILEEQ LIKEAFILSD LYNIGEIAAV ELLLIGEQQQ PTFHGLTRGL VAILLYWDGK SCMAESLLHL IQARKGKTFT L DHSPEVVS MVTRFTDDLM EQGLTNKILT LISQIDVNNE FDKLKKERGL GNKKHRKEVS DLIKECQQSL AHSLYSWSCQ TP LNREDTL LLIGYLEKVT VEGDGSLDKV NLTLLMSLLY CLDVGFLEQG TDDREELMKQ ASMFMDRQYI AAIHNRLQNT QPW KSPGMQ ATVRLAWALA LRGISQFSEV LEFSEADEPM AEIAIGGNVF LFLTEAVVGS ESFCTDEFFI RRIHKLVTDF PTLM PMKVK QLRNRAEEDA RLIQMSMQMG NEPPASLRRD LEHLLLLIGE LYRKDPFHLE LALEYWCPTE PLQSTSLMGS FLGVA HQRP PQRQVLLSKF VRQMSDLLPA TLYLPYLKML RGLASGPQCA HYCFSLLKAN GGSSAENLQA AGGSPVSWDH FFHSLM LYH EHLRRDLPNT DNIHQRHPPL RGITQRELDG LIACLQLTCT IIDWSESARL ALCEHAQWMP VVVILGLLQC SIPPLLK AE LLKTLAAFGK SPEIAASLWQ SLEYTQILQT VRATGLRQGV GIEVELNEIE SRCEEYPLTR AFCQLISTLV ESSFPTNL G AGLRAPGFEP YLQFLRDTVF LRYRTRAYRR AAEKWEVAEA VLDVFYKLLK DYEPQPEDFV DQYVELQGEE RVAFKPPGF SLMHHLLNES PMLELCLSLM EEGVTQLDTY APFPGKKHLE KAVAYCFMLL NLTLQKENRF MDLLRESHLS MIVTPLEQLL QGINPRSKK ADNVVNIARY LCHGNSNAEL AFESAKILCS ISCNSKIQEK IVGDFTQDQN VSQKLMVGFV SCLDSEEAEE L LDSEKEAE DQVKQTNIRY MTKIHILNLL ITSLEMKAPN LAMFLLGYEL KKPVSTTNLQ DSGVLGCPRT CLHSILDILR KG TDVRAGP VAVWDTPHLA ELCYQVIYQL CACADTSGPT MRYLRTSQDF LFSQLQHLPF SVEESEISAM NQMSWLMKTA TIE LRITSL NRQRSHTQRL LHLLLDDMPT RPYSADGEGG MEDESRSLSG FLHFDTTSKV RRKILRILDS IQFSNEIPEP LQLD FFDRS QIEQVIANCE HKNRRGQTVC NVKLLHRVLV AEVNALQGMA AIGQRPLLME EINTILQYVV ERNKLLQCLH AKRHA LESW RQLVEIILTA CPQDLIPTEH RQLIIRDLLQ DLHVKILDDD AAQELMPIVA GAVFTLTAHL SQSVRTELKQ PMTASG LGQ SQYVQMLDGS FAAPPGTENI SAGFASIGDS SLHMILRNLL EFILKTGGGF QRVRAHLYGS LLYYLQIAQR PDEPDTL ES AHKSMWERLT APEDVFSKLQ RDNLSIFESY GTALMEVVCR DACDGHDIGR MLALALLDRI VSVDRQQQWL LYLSNSGY L KVLVDSLAED DVVLRNLLTP QPPLLKALYI YESKMAFLTR VAKSSQGAIE LLRSGVIVRL AQCQVYDMRP ETDPHGVFG MRETPVFIPA PVERYRQILL PALQICQLIL TSSTAQHLQA AGQVLQFLVA HSDTIQAILR SQEGSLGSLQ ELALLTGIIS KAALPGVLN ELDIGLNDGS MMELQGHIGR FQRQCLALLN RFGGSDRLRQ LSLQDDSSRL DGVSKKDDME LAMQQICSNV M EYCQALMI QNSPSFQQTV CLFTPSLKES ASRDGTRQDS QVSILPSWRL PSLGVVIHLL KQSANNFFTY YDIHRQSVGK LQ NVEQLPP DEIKELCQSE MPVGADKIST TQKYGLARRR LVKLINSRAK LLSLCSYIIE TCLYILWRHL EYYLLHCTTS DSQ DPVFSN MTFGNRRFQD TFNTDPNMDP RNLRQNKVSQ QDVDTLLREG ANSFGESLQK RLLDIESLYC KVRSRHSFIQ ALVR RIRGL LRVSRV

Macromolecule #2: Nuclear pore complex protein Nup85

• Macromolecule: Name: Nuclear pore complex protein Nup85 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 75.160047 KDa

Sequence

String:

MEELDVDPAE TPIPGLGQQN RHIGFSWGPG DLLLYETLYQ KQGNSETAAR CPFMYLVRSD EDIYSPVLRK LFNESHSIFV GLQKSAEEA SGKSRKAQLV QVSRNYRSVL RACMEEMHTL SESTRETAQK YISQISILSA MELSWNLCEI LFIESAPAGP L LILLLEWV RLHVCEVDNI VQDVLRSEKP TEHEKFWDGV TGYVLQGRMN EARQLLAKEA STSASARSMC RVLDDLLKKM PM LHTGGTQ TLTEFELKWQ HWREECERHL QNGTFSSNVH MEAVCRVLLG DEEVLLEKRD LMTTWYHFLV SRLLFKHPTV KPT ELHFYA QSSLDMFLAG DSCPEPLDNI LLAAFEFDIH QVIKEFSIVS SNWWFVAHLT DLLDHCQLFQ AHNLYFGANM REFL LLDYA SGLFSHHSLW QLGVDYFDYC PNLGREYLKL HMERIPLSTE KKALKALRIC EQRQMTEQVR SICKTMAMQS LCNRR LGSA LSWSIRAKDA AFATLISDRF LKEYCERGNF TDLDLIDNLG SAMLLSDRLT FLGKYREFHR MYSQEQFSEA ASLLLS LMT ARIAPCSFWL TLLLDALPLL EQKQVIFSAE QTYELMRCLE DRMAAKLEST SPDEIQKQDS SIDNTKVEML RLALARN LA RAIVTEGALQ E

Macromolecule #3: MGC154553 protein

• Macromolecule: Name: MGC154553 protein / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 41.744512 KDa

Sequence

String:

MADKFAAKFV SHKISRTRWR PVSASSLQQP DVFATGSWDN EENKVCVWAT SDFGATSLDE EYQGDPKQLC DIKHPGDVMD MQFLDKERI VTGSSTGTVT IFRHHENNQT LSVNQRWEQA HYHVGSNMRA PCTAIVCSSP EIVSVGEDGR INCFRAESRD V LRTIDDAD SSTMHGVTFL RTTEILTVNS VGQLKLWDLR KQGNDPTQIF SVTGERVPLH CVDRHPNQQH VVATGGQDGM LC IWDVRHG KMPMSLLNAH EAEMWEVHFH PSNPDHLFTC SEDGSLWHWD ASADSEKPTF LLGGRSTFNI SRSSIAPPNA NQS LACAWL STDPTKGQLE ITNLLPSSTL SVNSLDVLGQ NLVCGTDAEA IYVTRRLFS

Macromolecule #4: Nucleoporin SEH1-B

• Macromolecule: Name: Nucleoporin SEH1-B / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 39.798602 KDa

Sequence

String:

MFVARSIAAD HKDLIHDVSF DFHGRRMATC SSDQSVKVWD KSENVNWHCT ASWKTHSGSV WRVTWAHPEF GQVLASCSFD RTAAVWEEI VGESNDKLRG QSHWVKRTTL VDSRTSVTDV KFAPKHMGLM LATCSADGVV RIYEAPDVMN LSQWSLQHEI S CKLSCSCI SWNPSSSRAH SPMIAVGSDD SSPNIMGKVQ IYEYNENTRK YAKAETLMSV SDPVHDIAFA PNLGRSFHIL AV ATKDVRI FTMKPLRKEL SSSGGVTKFE NHTVAQFDNH NSQVWRVSWN ITGTVLASSG DDGTVRLWKA NYMDNWKCIG VLK GDGNPV GNSFQGIFGS SIGSASHGLQ NSVNGTSTSG RKHS

Macromolecule #5: outer Nup160

• Macromolecule: Name: outer Nup160 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 162.658234 KDa

Sequence

String:

MAAAERHMTP FQAIDWAGSI TLPMVQRVGG FTRAIMAASV NLERSYMELI GAERETSRRN FRDLSLRPDV NLVIGGPKYA DCAGGYCYS ESSSLLSATR NRFLHWTSYA DTLELVEISL DINLVNNAVR LRILNCSILP GGVHICETPN NIVVLILTNQ T VHRLILPH PSRMYRSEII SDSHIQSIFT DIGKTNFHDP SNTYVIPAIP GRAPNTTAST AWLSSDGEAL FALPSISGGI LV IKMPPHD MEGLVTIAEL KQSSVMQRLL TGWMPSSIRG DQGPAHLPVS LAVHTLDHDS YLFALCQDHK LRMWSYKDQM CLM VADMLE YVPVSKDIRQ TAGTGHKLRL AFSETLGILY LGVYLHTPKQ GQFCVFQLMC AESNRYSLDH ISSIFTNQET LIDF TFTLT SMDIWALWLD DDNQTVVKHI NFEENQAGQW NPVFVNPLPE DDLAISDEQE PQEAYLECLF APGRFTIAAV QKAIQ ILRK GSGRVLDLSW EELRKDVTLT VENEIQNAVI DYDVSQEEFR QINIENWCKF YTCCLQYQET LSRPLALLVH PDTNMV CLL RKGFLSFLAP CSLVEHLYLV PAEHLLTVDE SVISDDIDAA SDIVNLIQCL RMIADYISED MAYLMESACC HLQSPER VA EQILEDLIAN DIDNIMENIQ NKLQDTRNPI RAIGFLLQNM DYETNADMEQ PQPNTRLNLS TLYGSITASS VVCQAICK I SATRFLICRD LLILQHLLLR LGDMALIGAG QLLHSQQELI PRAAQLLLSY YMIRWGSQCL ACAVPVDILE SNLQHLSVL ELSDSQVEKR RYTSGIQTIV ELFFEDVARK HFPHVFIQSG ASQLQEPLNW SDLIKRITNY LLQLLWPSNP NFQFAECLMR NCQYTQLQE YVRLLLPWCQ VNVGSCHFML AQCYLVAGEG HKALDCFSQA ASEVEREDFL EKLIRVEEGE SVSPRLQYYN R VLRLLEDV GLPELVIQLA TIAIGEASDD WRSQAALRTR IFKHHLDMGH NNQAYDALTQ IPDPSRQLDC LRQLVVVLCE RS QLQDLVE FPYVNLHNEV VGIIESRARA VDLMTHNYYE LLYAFHIYRH NYRKAGSVMF EYGMRLGREV RTLRGLQKQV NSY LACLNC LRLIRPEYAW IVQPVSGAVY ERPGASPKRN YDGESSAVPS SSQIEILELR DLEKEYVLAQ TRLTLAKHNP STAA IAGSS AAEEMVALLV QAGLFDTAIS LCQTFKLALT SVFEGLACKC IRLQQGGEAA QAEAWEWLAA NQLATVITTK ESSAT DEAW RLMISYLDKY EAKNTLYHHC IINKLLSHGV PLPNWLINRY KAMDAAELLR LYLKYDLLEE AAELVLEYVD ALLGKG HQY FGIQAPLSAT SQLVWFPYSA IDHLRQALGE NESNQHNQAI LSKLQRKMDE YFQKLKKATD DYKKLVQKPL RA

Macromolecule #6: MGC83926 protein

• Macromolecule: Name: MGC83926 protein / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 36.588625 KDa

Sequence

String:

MKQDSASNAT YTVDCEDYVH VVEFNPFDSG EAGSLLAYGG ISYVVIASCR FQEEDSTVEG IEFKTLKTFH HGERVVAIAW SPETRCDAL LPLLRFATAA GDKKIRIFTS DFQDKNEYKV IEGHSGYIND LVFCSPEGTD IASVGDDHTC RIWDLDGKQI A MFILRSPG MSVAWHPEGA FKLMVAEKTG TIRFYDLTTH QAILSLESVQ VPLMSADWCV RNTLRIGAVA GNDWIIWEMP RS SYPQDNK PAHADRARMF RWSKCNENVF ATTGYPGKMK SQIAIHHLAH PQPILIGTAP VGSGLSWHRR LPLCVVGGYR KLF FWLTEM

Macromolecule #7: Nuclear pore complex protein Nup98-Nup96

• Macromolecule: Name: Nuclear pore complex protein Nup98-Nup96 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 191.067188 KDa

Sequence

String:

MFNKTFGSPF GTGNGAFGAT STFGQTTGFG TTPATAFGSA GFGTNTSTGG LFGNAQTKPG SLFGTNTFNQ PATSSASSGF GFGTSTGTT NSLFGSTNTS NSLFSAQNNA FGQTKPTTFG NFGTNASTGG LFGTTNTANP FGGASASLFG ASTFTSAPTG T TIKFNPPS GTDTMAKGGV TTNISTKHQC ITAMKEYESK SLEELRLEDY QANRKGPQNP VGAPTGTGLF GTPAATSSTA TG MFGSTAA NSFSFAGNKT TFGTAATGAF GTNTGALFGQ PANQPAASLF NKPFGNATTT QTTGFSFGNT STLGQPQTST MGL FGSNQA TQPGGLFGTT TNTNAANAFG TGASLFGQPN ATTFGGSSVW FGSNTAGTGL FGNKPATGTI GPSLGTSFGT ALNP GQTSL FGGNQPKLTG TLGTGAFGGT GFNSTSTGLF GAPQAAATTL TDFNASAAQQ MFLQQQYNAL RYSPFPDSPL FMNPI SDPK KKEELLKPTN PAAQKAVLTP THYKLTPRPA ARVRPKALQY SGAAKNQLFD ALDDDVPVLS SGVFMPKKSI KQLDLK YLK NSSLFSQKSK DLDEVVPPTP ETQDRSVENH HADEENDEEA ATTYPSPFSR PLPQSQEKKQ FNNMDDTIVA LNMRKSG RI GLENSSNDVS FNEDSLRDTE ILDASPHPAG ITLMRDSYYT IPSMEELARS VDENGECIVD GFTIGREGFG SIYFDGII N LTNLDLDSIV HIRRKEVIVY VDDQNKPPLG EGLNRSAQVT LDEVWPVDKT TRCTITSPER LNEMNYKAKL ENASRKQGA QFVDYRPESG SWVFKVNHFS KYGLQDSDEE DDQLNNAEAK KLKAAPVPPQ GKPPPLQQAT LPGKVTPPPQ SPAVDQLDRV LELDSDMAD ITQDQDLDSV AEEQDITEEQ EPLSASSHIA SSLGINPHAL QVMKASLLLE EEDGEMINRF SSFPSSMDPY P DVRSPRLF PSSHAKRTSS MGLLQSKFAS PSISRISETA QGSHSPRILP VTPWSVPAPL APTFVIPRPA PETHLRTVGT RR QQELVPL EKSVTHGRGS LLIDMGLFMG RSFRVGWGPN WTLVHNGDKL TERLNAEEDQ NMDTIDYGFL PKPTSAKSLT ESP FKVHME KLSLEQKSRE LQSYLMPLEI ELKNSSVDRS AQCPHFKPNA GVAAIHDYAG WVRNLSNEAG ELEAVVKQWG LTWT LCESL WGQLKELEAS LDEPNEYVRN LERRKAFSHW LAHTAEERIE EEVSLYGPER HVEAVFSFLT GGRISDACRL AQKSG DHRL SLLLSQMVGS QEMRELISLQ LVDWNKLQVD HYIQEERLRV FCLLSGTPVW RSSDNRSINV CSQLDWKRTL AVHLWY MLP PTATIAQALR LYERAFQEHE EGEPYACYPL PPYLEDCSIS LGDEPSAKFS SLQRDVCVHL LKLYSERQYD LCQLLDP SS VTPDPLDYRL SWHLWMVLQA LNYTHLSEHR QGTLHASYAA QLENVGLWEW AIFVLLHIPH PHIREAGVRE LLNRQCVV R ESPESLAKEN FLIHRLCVPA QWVHEAKAIR SRRDGDRHKE ALYLLKGHQW NPCHKLVTRH LAADAVINEN YRYLQSFLG ELSNPEHCKH IQDWETAGKV YLDYIRVIDM LNLIQQDESS GCELEKLHTK VMSLCKWVEL IHCYTAKDRL AQSEMAKRVA NILRVVLSL QQPPESMSDS SEPRVPLRLL APHIGRLPMP EDYALEELRG LTQSYLRELI CDS

Macromolecule #8: Protein SEC13 homolog

• Macromolecule: Name: Protein SEC13 homolog / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 35.361309 KDa

Sequence

String:

MVSVINTVDT SHEDMIHDAQ MDYYGIRLAT CSSDRSVKIF DVKNGGQILI ADLRGHEGPV WQVAWAHPMY GNILASCSYD RKVIIWKEE NGTWEKTYEY TGHDSSVNSV CWAPHDFGLL LACGSSDGAI SILTYTGDGP WEVKKISNAH TIGCNAVSWA P SVVPGSLV DQPSSQKPNY IKRFVSGGCD NLVKIWREED GQWKEDQKLE AHSDWVRDVA WAPSIGLPTS TIASCSQDGR VY IWTSDDA ATNCWTPKLL HKFNDVVWHV SWSITANILA VSGGDNKVTL WKESVDGQWA CISDVNKGQG SVSTVTDGQL NEQ

Macromolecule #9: Nuclear pore complex protein

• Macromolecule: Name: Nuclear pore complex protein / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 105.398547 KDa

Sequence

String:

MDMLSPVVRE AEVSRAARRQ SSNRKNPADE SWSNATPTRG PSSRTTGQTL FRQHMTPQTW NSSRPPDVSA ILGTVGRSPR LLQTPGRLA NLSMMSNPDD SVWTTTFSPG RTGMYTTLDS PSFTEDITLS AVMLQEEDPG EAATMSMYPD FLKSFLEHPS S AVFELIEQ YEATCNTQIT LLKKIVKRVT PGQQKFSKTA SILWLLQQEM VTWRLIAALY RDRIQSALEE ENMFEIAAPN AS EKTIVDK LFQRDTLVRQ SQLVVDWLES IAKDEVGDFS DNIEYYAKSV YWENTLHTLK QRSMLSLGSS RPLVSELDPD API RQKLPL DDLDREDDIR LLKYLFTLIR AGMTDEAQRL CKRCGQAWRA ATLEGWKLYH DANINGGTEL QAVEGNPYRC VWKT CCWRM AEDEQFNKYE RAIYATLSGN LKQLLPVCES WEDTVWAHFK VMVDSLVEQE IRASIISFNE ANELPREYLE ANWTL DSVF EELQATDKKR VLEENREHYH IIQKFVILAD VDGLMDEFSE WLSNGKNLLL GHLLRFMTHL LLFFRTLGLQ AKEEVS VEV LKTYIQRLIN EKQIELIAFY VSHLPQELAI SQYAVFLENI TDPDQRQRCL ELAKEAGLDV ASITKTVVEN TRKKDAG EF AHHDFAPALD SGTSEEDRAK IDVIDWLVFD PAQRAEALKQ SNAIMRKFLA SKKHEAAKEV FAKIPQDSIA EIYSQWEE Q AMDSALPAED DNAIREHLCI RAYLESHEAF NEWFKHINSP PQKPTLVGQA SFTEKVAHEH KEKKYEMDFG IWKGHLDAL TSDVKEKIYN VLLFVDGGWM VDVREDTEED PERSHQMVLL RRLCLPMMCF LLHTVLHNTK QYKDCLRLAD IVSSENQKLY TVFSKTEMR NLLQKLRESS LMLLDLQLDP LGYEIQS

Macromolecule #10: nuclear pore complex protein Nup133

• Macromolecule: Name: nuclear pore complex protein Nup133 / type: protein_or_peptide / ID: 10 ; Details: This entity was identical with chain j. However, N-terminal region cannot be assigned due to disorder.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 111.296109 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)YFRE VSQMEIIFEC LVDKEEADLE STSIDSVEWA NIVVNVNTIL KDMLHVACQY RQSKNSLYKN ESGIQE PEH VPWTASSGTA GIRSVVTRQH GIILKVYPQA DSGLRTILIE QLAALLNYLL DDYVTQLKSI DKLANEERYN ILEMEYA QK RSELLSPLLI LGQYAWASNL AEKYCDFDIL VQICEMTDNQ SRLQRYMTLF AEQNFSDFLF RWYLEKGKRG KLLSQPAS Q HGQLAAFLQA HDHLSWLHEL NSQEFEKAHR TLQTLANMET RYFCKKKTLL GLSKLAALAS DFQEDVLQEK VEEIAEQEH FLLHQETLPK KLLEEKQLDL NAMPVLAPFQ LIQLYVCEEN KRANENDFMK ALDLLEYIGD DSEVDVEELK LEILCKAIKR DEWSATDGK DDPIEATKDS IFVKVLQNLL NKGIELKGYL PKAETLLQSE ELNSLKTNSY FEFSLKANYE CYMKMQS

Macromolecule #11: Nup358 complex, clamps

• Macromolecule: Name: Nup358 complex, clamps / type: protein_or_peptide / ID: 11 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 322.784344 KDa

Sequence

String:

MRRSKAEIQR YVENAQNSAS SPREKSMKGF LFARLYYEAK EYELAKRSVS SYISVQERDP KAHRFLGQLF EIEGNVEKAV GCYKRSLEL NPTQKDLTLR IAELICTLNI KDGRAEYWVE RASKLFPGSP EIYRLKEQLL SSQGEAGWNQ LFDLIQAELF A RPNDVYVN LKLVDLFLSN QRLEEAVLHC LKPERRALRT DIEWCSCVVR VFKEYLASKQ GQKNTNMRMI TKELLLAQCD VV FLTLSKK DVQKSKEALE RFDQALLSVK QSVSGTDASD LSVTFYEMRG HYYMHAGTLL LKMAQSCEVQ WKALIEPAAL CYL LAYQVP KPKSKPVKGD DNGQGFLEEL AFDRQSKSGH LLLTLSHGKQ NFISEIIETF ANQCGQSILL KFLFEDNLSM QDSF MGSDD ISYVENRVPD LSELSQHDNG SLRIHNGDLQ HLTWLGLQWH FLSTLPPLRK WLKQIFPRVP QETSRLESNI PESIC LLDL EVFLLAVVQT SYLQLQDNNT TADPNRPRCL PLPICKQLFT DRQRSWWDAV YSLITKKALP GTSAKLRSVI QHDLTT LRA QEKHGLQPAV LVNWARGLHK TGYSLNSFYD QKEYMGRCVH YWKKLLPLLD LVKQKKSIPE PVDPLFKHFH NKDIKVS EV KDLEDEACIA FATLDLVDGK TEDAIIAFES VKNVVAYWNL ALIYQRKAEE IENDCLPAEE QEEFQECLLK CKGFLKMI C DEYSAYPSIA TSLPVPVETV FEMLDSVKQS LGEAMDDHSP AFMENHSVLT TSAIKHSTPS PTKLTISPSK SARFSPKTP PRWAEDQKSL LEVLCNKVEA LKKEVQELKH NNSNANVSPH RWPNEGFESD TVADSYQGTQ NFYTVPLTVS TSGPAAYYGQ SPAYNSQHL LRPAANITPT KTSVYAMNRL PPQQHMYTYP QQMHTPPTQQ SSAGCVFPQE IYGPPLRFES PAAAILSPHN E EFYNYNVP PASTNPPLPE PGYFTKPSTA MQHSKQEVPK VSDFGKGCLG QSTSEGQKPS PFTVPMQSTP ASSTFKFNSN FK SNDGDFT FSSSHAGASS AYTGSESLLG LLTSDRPTQE QGKKSDFENI ASDEKNMFRF GEKSFSPGFT VTGTQSQDKN PLV FGQSEN IFTFKTPGKS TFKPPTFGTQ TKDAHNHSVE SDAGSEHVAD DDGPHFEPII PLPEKVEVKT GEEDEEEMFC NRAK LFRFD AETKEWKERG IGNVKILRHR LSGKIRLLMR REQVLKICAN HYINADMKLK PNATSDKSYV WHAYDYADEM PKPEQ LAIR FKTVDEAAHF KAKFEEAQRL LAMAEAPAIS AQHKNAKDNL KLDASKVKEA PLPFGSQFIL KRGEWQCDCC LATNAP TST SCVCCQTPNK NKSSSISSVC ISAPSFTFVK ESATNKLAFG QQLLKDKDQW TCSKCSQKND AGVSHCSSCQ TQSQAKA GI SQPNIASSGF TNNTSAQGDN LAAVFGKKAG QWDCDVCYVR NEPSANKCIS CQNTKPLSKV SGTQAASFSF AAGADNSQ K NFGAQFAKKE GQWDCDACYV RNEPLATKCI SCQNTKLLSK TTGTQAASFS FAAAADNSQK NFGGQFAKKE GQWDCSSCL VRNEASAPNC VACHSANPQI TNKDVVPPAL TPSGFKFGHN AEVGKTQQSL SAMFSCKQGQ WECSTCLVIN DAAKDTCAAC QAAKPGSSA SQSKEVPSTF GIKANSSQNF GQPAAGFNCG FSAKGFKFGI SDEKASASNF TFKAPATNEE TKMVKDGFNF P VSAGSLSF KFGISEPDKT KEMSTGFMKG TSTNNKGSET AETTAQAEKI QQSPDKVLGQ SVQSFSFADI AKSTEGIEFG KA DPNFKGF SGAGQKLFTS SNQVNASNAQ EAADDLYKTE ERDDIHFEPI VQLPDKVDLI TGEEDEKTLY SQRVKLYRFD ATS GQWKER GVGNLKILKN EVNGKLRVLM RREQVLKVCA NHWITTTMNL KPLTGSDRAW MWLANDFSEG DAKLEQLAVK FKTP EQAEE FKIKFDQCQC LLLDIPLQTP HKLVDTGRTA HLIQKAEEMK TGLKDLKTFL TDKAKPLDDS NAINTTDLEK QALAD GTEP TYEWDTYDMR GDAHEETLDD SVYASPLASS PEKKNLFRFG DLSTSGFNFS FQPEPSPSKS PTKLNHSRVS VGTDEE SDV TQEEERDGQY FEPVVPLPDL VEVTSGEENE QAIFCHRAKL YRFDKDSNQW KERGIGDLKI LQRLDNKSAR VVMRRDQ VL KLCANHRITT DINLQPMKGA ERAWVWTAHD FSEGEGKVEC FAVRFKLQEA ADLFKEVFEE AKEAQAKDCL LTPVSSRG T TPRAASCGKA AIAILEETTK ERTDQQPEED TSLTEASTPS PTDQPAKALV SPANFTFGSD VVKNIFGSEK QVPFAFGNT SSTGSLFGFS FNASQSQGQQ VQKQPPKVTL DFNATFKDAE TTNALQKPSQ SSGQSPIVSS LSSSSSSSSS TLMQPMPARD KAADVPDAD SSSDVLIVYV ATPTPEQKAL AETLLLPLTF FCYKNKPGYV SDESDIDDED FETAVKNLNG ILYTEDKKDK A SSRLSGCS KEPTAESDQD CIIVWEKKPT PEEKAKADSL KLPPTFFCGL GSDTDEDKDN LEDFDTEVRK VKEAKGVPEA DV TSSPEAA IVSAAETSVS LPPKQEPDST TSISQEPVDL SSKQELPKTD SKGFSTPSFS FGLGEVSGVS FADLASTNSG DFA FGSKDT NFQWANTGAA VFGTLSQNKK GEDADGSDEE VVHSDDVHFE PIVSLPEVEV KSGEEDEEIL FKERAKLYRW DRAV GQWKE RGVGDIKILF HKEKGYYRVL MRRDQVLKVC ANHVISTEIK ISTLSTSNNS LVWTATDYSD GEGKVEQLAV RFKTK ELTD SFQNKFEECQ HNLQEESNPQ H

Macromolecule #12: Nuclear pore complex protein Nup93

• Macromolecule: Name: Nuclear pore complex protein Nup93 / type: protein_or_peptide / ID: 12 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 93.565156 KDa

Sequence

String:

MDGEGFGELL QQAEQLAAET EGVTELPHVE RNLQEIQQAG ERLRSKTMTR TSQESANVKA SVLLGSRGLD ISHISQRLES LSAATTFEP LEPVKDTDIQ GFLKNEKDNA LLSAIEESRK RTFVMAEEYH RESMLVEWEQ VKQRVLHTLL ASGEDALDFT Q ESETSYIS ESGAPGRSSL DNVEMAYARQ MYMYNEKVVS GHLQPSLVDL CTEAAERLDD KNVSDLWVMV KQMTDVPLIP AS DTLKSRC SGQMQMAFVR QALNYLEQSY KNYTLISVFA NLQQAQLGGV PGTYNLVRSF LNIRLPTPIP GLQDGEIEGY PVW ALIYYC MRCGDLMAAQ QVVNRAQHQL GDFKNCFQEY IHNKDRRLSP TTENKLRLHY RRAVRASTDP YKRAVYCIIG RCDV SDNHS EVADKTEDYL WLKLSQVCFE DEANSSPQDR LTLPQFQKQL FEDYGESHFA VNQQPYLYFQ VLFLTAQFEA AIAFL FRLE RTRCHAVHVA LALFELKLLL KSTGQSAQLL SQEPGEPQGV RRLNFIRLLM LYTRKFEPTD PREALQYFYF LRNEKD NQG ESMFLRCVSE LVIESREFDM LLGKLEKDGS RKPGAIDKFT RDTKTIINKV ASVAENKGLF EEAAKLYDLA KNPDKVL EL TNKLLSPVVS QISAPQSNRE RLKNMALAIA ERYKSQGVSA EKSINSTFYL LLDLITFFDE YHAGHIDLSF DVIERLKL V PLSQDSVEER VAAFRNFSDE IRHNLSEILL ATMNILFTQY KRLKGSGPTT LGRPQRVQED KDSVLRSQAR ALITFAGMI PYRMSGDTNA RLVQMEVLMN

Macromolecule #13: Nup155-prov protein

• Macromolecule: Name: Nup155-prov protein / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 154.922422 KDa

Sequence

String:

MPSATPGAPA ASAAMQEALE GAGRIIDRLL QEDRAYPDLS ELLNVPVHTC PTISGVSEMD YPLQSPGLLT IPSLPEISAI RRVPLPPEL IEQFGHMQCN CMMGVFPEIS RAWLTIDSDI FMWNYEDGGD LAYYDGLSET ILSVGLVKPK TGIFQPHIRF L LVLSTPVD IVILGLSFAN LQPGNLNDSI SGGMQLLPDP LYSLPTDNTY LLSITSTDNG RIFLSGKDGC LYEVEYQAEA GW FSQRCRK INHSKSSLSF LVPSVLQFAF SEDDPIVQIA IDNSRNILYT RSEKGVIQVY DLGVDGHGMS RVASVSQNSL VSA AGNIAR TIDRNVFKPI IHISVIEMSE SVNCHLLAVT HTGVRFYFST VPFKQPTARP CMLALVHVRL PPGFSASSNV EKPS KVHKA LYNNGVLLMA ASENEDNDML WCINRDSFPF QKPMMETQVT TQVDGHSWAL SAVDEQKADK IVTPLNKDLI PLTDS PVII QQHMIPPKRF VLLSAQGSHI FYKLRPVDQL RHLLVSNSGG DGEEIERFFK LHQENQACAT CLILACSSAA SDREVS SWA ARAFFRYGGE AQLRVQSALH APSNVGPIFG SPLPVASPIP VGSPMPNPSF LGTPTQGACP PNVSTPAYGV ATPAPQP PA VPGMMGTEIV FSGKHNGICI YFCRIIGNIW DGSVAVENTF QSGNREVTAI DSSVTPQHLE SVLKELKGLL EFLDRYSQ F TAGSLGNPGF GTPANRQQRL VSLGRPDIGS SQQAQQELQR KYHTEAQLAE QFSLQGIHQL VRKMCQALAL WKLLCEHQF SLVVSDLQKE LQEQLKITTF KDLVIRDKEL TGALTASLIS CYIRDNASVD GISYRLQEVC PLLYSTDDAV CSKANELLQR SRHVPNKQE KERMLRESLK EYQKISQQVD LPNVCAQYRQ VRFYEGVVEL CLSAAEKKDP QGLGLHFHKN GEPEEDMAGL Q AFQERLNS YKCITDTLQE LVNQSKAAPQ SPSVPKKPGP PVLSSDPNML SNEEAGIHFE QMLKLAQRSA DELFNIALFN WL IQADLTD KLLELNSPFL EPHLVRMAKL DQNKVRYMDL LWRYYEKNRN FSNAARVVAK LADMHSPEIS LKQRLEYISR AIL SAKSST TMSTLAADGE FLHELEEKLE VARIQLQIQE TLTRQYSHHS TVGDAVSQLD SQLMDITKMF GQYADPFRLS ECKL AIIHC AGHSDPILVQ TLWQDIIDKE LSDSMGNSSV DRMQSLHLKI TSLGKIYAST PRYFPLEFLV KYLEQQVCNF SWDAG FVTY TMQEINVPVP KLLEVYDHLF KARDPWWSRM KKPLHLLESI YILLSGYVQE PSKVPSYERR RFTTLCLDAI SCYLVE LQS MDPAPALLNT VSNFKSLQAK LERLS

Macromolecule #14: Nup98

• Macromolecule: Name: Nup98 / type: protein_or_peptide / ID: 14 ; Details: The putative protein Nup98 was from the same gene of the chains g/G, whose corresponding peptide is post-translationally cleaved into two separate proteins.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 13.719902 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #15: Nucleoporin Nup88A

• Macromolecule: Name: Nucleoporin Nup88A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 82.570148 KDa

Sequence

String:

MAAGEHWQSA LSEHALFSSL KERLRDVEEL REEGERRASK DKQLRNLLCV LDGDLLVWDA EECAFHTVTL RSLSVESTGN SSSGEQKLL CTNPPLFDVN EVLLSPTQHH VALVGSKGVM VLEIPKRWGK KSEFEGGEKT VNCRTIPIAE RIFTSSTSLL L KQAIWYPS ETQEPHLILL TSDNILRLYN LQDLFTPVKV ISLSSAEEDT TLPHNGRSYK ASLGETAVAC DFGPLAVLPK GF GQHSKED TVAYPLYILY ETGETYLMYI DLQKSNITVG KLLGPLPMYP AAEDNYGYDA CALLCLPCVP NIIVIATESG LLY HCVVLE GEEDDEQTSN KSWNASCDLI PSLYVFECVE LELALKFATE EEESLELDFA CPIKLHRDPI CPSRYHCTHV AGVH SVGLT WFNKLEKFLS SGEEDKDSLQ ELAAEQKCLV EHILCTKPLR CRLPSPIQGF WIISDLFLGT SMICITCDFE CIVRP LLTT IRPPSPPLLC SQSDKSSTEI LPHVLADVKG PFEEHIRGIL RRNSANPLLL NSSSKDSSPP PEECLQLLSR ATQVFR EEY LLKQDLANEE IQRRVKLLIA QKEKQLEDLR YCREERKSLT ETAERLAEKF EEAKEKQEDL INRLKRILRS FHTQLPV LS ESERDMKKEL QATNQQLQQL GNSINQVNRK MSYQEKQMEK GKSPRKSSLT LSDYQKKNIK AVLKEHGEHI QEMVKQIN N IRNHVNF

Macromolecule #16: outer Nup133

• Macromolecule: Name: outer Nup133 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: African clawed frog (African clawed frog)
• Molecular weight: Theoretical: 127.55125 KDa

Sequence

String:

MFPSPRAQGM GSARRPFNSR LTGGRKALGP GVTASSSPSA LYSPVGRRVS ASGARSTPSR VYLHPAASET VNYNVQLFGS SLPVKVMEA LSNASADEPM AACIHEGGWA WLACNDRLII WKISHSSSAK LMVCKELPLP LSDSEWSADL VDICAQTGDP A AAQSVALM AATPEGSSRY WPNILHEGTY IESYTEFGSS LCAFVTAVKG NSFILSSEKN QLVRLTPDAS GKMNQRVLPQ GQ GMLSGIG RRVSTLFGIL SPAVESTLCS VLWDKGDCFY TLTDSSINKW DLDDTSESQV LNWDMSRVLR EYISDAIWGS ESD YDDIKA GININYLSLN QNCDGLVILS AAWHPGDNPC QIYYTLVTVK DEGYNISDEI TVEVTQFNPV FQARGMQLCQ LVVP NFSSQ ACYLYTQEMI FACSTGTGRS TLPQEKIPFE AQGDNIVGAG SCEGWPVFFI RKSGMLTVVA RETASVLPEH MEESL SSVS KSSRQAVVKD SRPDQIAHDD KTKHLKAAFL RYCRKDILGA QSMVDSLFSD SDMEPDDELD LAVNQISVDL IDDYPA SDP RWAESVPEEA AGFSNTSLIL LHQLEDKMKA HSFFVDFLHQ VGLFSRLSTC QTKGMLVATR LLLSEHAEKL SAAIVLK NH HAKLPVLVNS AIQLALDKRM CTVPQNLTAA DVYFREVSQM EIIFECLVDK EEADLESTSI DSVEWANIVV NVNTILKD M LHVACQYRQS KNSLYKNESG IQEPEHVPWT ASSGTAGIRS VVTRQHGIIL KVYPQADSGL RTILIEQLAA LLNYLLDDY VTQLKSIDKL ANEERYNILE MEYAQKRSEL LSPLLILGQY AWASNLAEKY CDFDILVQIC EMTDNQSRLQ RYMTLFAEQN FSDFLFRWY LEKGKRGKLL SQPASQHGQL AAFLQAHDHL SWLHELNSQE FEKAHRTLQT LANMETRYFC KKKTLLGLSK L AALASDFQ EDVLQEKVEE IAEQEHFLLH QETLPKKLLE EKQLDLNAMP VLAPFQLIQL YVCEENKRAN ENDFMKALDL LE YIGDDSE VDVEELKLEI LCKAIKRDEW SATDGKDDPI EATKDSIFVK VLQNLLNKGI ELKGYLPKAE TLLQSEELNS LKT NSYFEF SLKANYECYM KMQS

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.5 µm
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 75.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: EMDB MAP
EMDB ID:

0909

• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 1279270

Atomic model buiding 1

• Refinement: Space: REAL

Output model

PDB-7fik:
The cryo-EM structure of the CR subunit from X. laevis NPC