[English] 日本語
![](img/lk-miru.gif)
- EMDB-31428: Cryo-EM structure of the fibril formed by disaccharide-modified a... -
+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Cryo-EM structure of the fibril formed by disaccharide-modified amyloid-beta(1-42) | |||||||||
![]() | ||||||||||
![]() |
| |||||||||
![]() | amyloid fibril / PROTEIN FIBRIL | |||||||||
Function / homology | ![]() signaling receptor activator activity / Golgi-associated vesicle / clathrin-coated pit / axonogenesis / central nervous system development / heparin binding / growth cone / perikaryon / early endosome / membrane raft ...signaling receptor activator activity / Golgi-associated vesicle / clathrin-coated pit / axonogenesis / central nervous system development / heparin binding / growth cone / perikaryon / early endosome / membrane raft / signaling receptor binding / Golgi apparatus / cell surface / endoplasmic reticulum / extracellular region / nucleus / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||
![]() | Xia WC / Sun YP / Liu C | |||||||||
Funding support | 1 items
| |||||||||
![]() | ![]() Title: O-Glycosylation Induces Amyloid-beta To Form New Fibril Polymorphs Vulnerable for Degradation Authors: Liu D / Wei Q / Xia W / He C / Zhang Q / Huang L / Wang X / Sun Y / Ma Y / Zhang X / Wang Y / Shi X / Liu C / Dong S | |||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 4.4 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 9.4 KB 9.4 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 7.4 KB | Display | ![]() |
Images | ![]() | 80.4 KB | ||
Filedesc metadata | ![]() | 4.6 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 409.2 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 408.7 KB | Display | |
Data in XML | ![]() | 9.9 KB | Display | |
Data in CIF | ![]() | 12.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7f29MC M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-
Sample components
-Entire : Cryo-EM structure of the fibril formed by disaccharide-modified a...
Entire | Name: Cryo-EM structure of the fibril formed by disaccharide-modified amyloid-beta(1-42) |
---|---|
Components |
|
-Supramolecule #1: Cryo-EM structure of the fibril formed by disaccharide-modified a...
Supramolecule | Name: Cryo-EM structure of the fibril formed by disaccharide-modified amyloid-beta(1-42) type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1 |
---|---|
Source (natural) | Organism: ![]() |
-Macromolecule #1: Amyloid-beta A4 protein
Macromolecule | Name: Amyloid-beta A4 protein / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 3.90044 KDa |
Sequence | String: HDSGYEVHHQ KLVFFAEDVG SNKGAIIGLM VGGVVIA UniProtKB: Amyloid-beta A4 protein |
-Macromolecule #3: ACETIC ACID
Macromolecule | Name: ACETIC ACID / type: ligand / ID: 3 / Number of copies: 6 / Formula: ACY |
---|---|
Molecular weight | Theoretical: 60.052 Da |
Chemical component information | ![]() ChemComp-ACY: |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | helical reconstruction |
Aggregation state | filament |
-
Sample preparation
Buffer | pH: 4 |
---|---|
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 55.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |