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- EMDB-3128: Structure of a cross-beta amyloid fibril from IGSNVVTWYQQL peptid... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-3128 | |||||||||
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Title | Structure of a cross-beta amyloid fibril from IGSNVVTWYQQL peptide of AL-DIA immunoglobulin light chain by cryo-EM and fiber diffraction | |||||||||
![]() | Reconstruction morphology 1 of a left-handed amyloid-like fibril of the IGSNVVTWYQQL fragment of an immunoglobulin light chain | |||||||||
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![]() | AL amyloidosis / cryo electron microscopy / steric zipper / three dimensional reconstruction | |||||||||
Biological species | ![]() | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 8.3 Å | |||||||||
![]() | Schmidt A / Annamalai K / Schmidt M / Grigorieff N / Fandrich M | |||||||||
![]() | ![]() Title: Cryo-EM reveals the steric zipper structure of a light chain-derived amyloid fibril. Authors: Andreas Schmidt / Karthikeyan Annamalai / Matthias Schmidt / Nikolaus Grigorieff / Marcus Fändrich / ![]() ![]() Abstract: Amyloid fibrils are proteinaceous aggregates associated with diseases in humans and animals. The fibrils are defined by intermolecular interactions between the fibril-forming polypeptide chains, but ...Amyloid fibrils are proteinaceous aggregates associated with diseases in humans and animals. The fibrils are defined by intermolecular interactions between the fibril-forming polypeptide chains, but it has so far remained difficult to reveal the assembly of the peptide subunits in a full-scale fibril. Using electron cryomicroscopy (cryo-EM), we present a reconstruction of a fibril formed from the pathogenic core of an amyloidogenic immunoglobulin (Ig) light chain. The fibril density shows a lattice-like assembly of face-to-face packed peptide dimers that corresponds to the structure of steric zippers in peptide crystals. Interpretation of the density map with a molecular model enabled us to identify the intermolecular interactions between the peptides and rationalize the hierarchical structure of the fibril based on simple chemical principles. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 2.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.8 KB 9.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 11.8 KB | Display | ![]() |
Images | ![]() | 16.8 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Reconstruction morphology 1 of a left-handed amyloid-like fibril of the IGSNVVTWYQQL fragment of an immunoglobulin light chain | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Amyloidogenic Fragment IGSNVVTWYQQL of Immunoglobulin Light Chain...
Entire | Name: Amyloidogenic Fragment IGSNVVTWYQQL of Immunoglobulin Light Chain of Human AL Patient |
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Components |
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-Supramolecule #1000: Amyloidogenic Fragment IGSNVVTWYQQL of Immunoglobulin Light Chain...
Supramolecule | Name: Amyloidogenic Fragment IGSNVVTWYQQL of Immunoglobulin Light Chain of Human AL Patient type: sample / ID: 1000 / Details: The sample forms long amyloid-like fibrils. / Number unique components: 1 |
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-Macromolecule #1: Immunoglobulin Light Chain
Macromolecule | Name: Immunoglobulin Light Chain / type: protein_or_peptide / ID: 1 / Recombinant expression: No / Database: NCBI |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | helical reconstruction |
Aggregation state | filament |
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Sample preparation
Concentration | 5.0 mg/mL |
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Buffer | pH: 8 / Details: 50mM Tris-HCL |
Grid | Details: C-flat 1.2/1.3-2C 400 mesh grids, glow discharged |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 50 % / Chamber temperature: 100 K / Instrument: GATAN CRYOPLUNGE 3 Method: Incubation of 0.014 mg/ml fibril solution on glow discharged holey carbon grid for 30 seconds and backside blotting for 4 seconds before plunging. |
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Electron microscopy
Microscope | FEI TECNAI F20 |
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Temperature | Average: 100 K |
Date | Nov 12, 2012 |
Image recording | Category: CCD / Film or detector model: FEI FALCON I (4k x 4k) / Number real images: 10 / Average electron dose: 25 e/Å2 / Bits/pixel: 16 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Calibrated magnification: 66350.7 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.0 mm / Nominal defocus max: 5.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 50000 |
Sample stage | Specimen holder model: GATAN LIQUID NITROGEN |
Experimental equipment | ![]() Model: Tecnai F20 / Image courtesy: FEI Company |