+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-31220 | ||||||||||||||||||
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Title | Asymmetric reconstruction of attachment protein sigma1 | ||||||||||||||||||
Map data | |||||||||||||||||||
Sample |
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Biological species | Mammalian orthoreovirus 3 Dearing | ||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.8 Å | ||||||||||||||||||
Authors | Pan M / Zhou ZH | ||||||||||||||||||
Funding support | United States, 5 items
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Citation | Journal: To Be Published Title: Asymmetric reconstruction of mammalian reovirus reveals interactions among RNA, transcriptional factor mu2 and capsid proteins Authors: Pan M / Alvarez-Cabrera AL / Kang JS / Wang L / Fan C / Zhou ZH | ||||||||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_31220.map.gz | 2 MB | EMDB map data format | |
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Header (meta data) | emd-31220-v30.xml emd-31220.xml | 8 KB 8 KB | Display Display | EMDB header |
Images | emd_31220.png | 46.5 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-31220 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-31220 | HTTPS FTP |
-Validation report
Summary document | emd_31220_validation.pdf.gz | 328.7 KB | Display | EMDB validaton report |
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Full document | emd_31220_full_validation.pdf.gz | 328.2 KB | Display | |
Data in XML | emd_31220_validation.xml.gz | 5.7 KB | Display | |
Data in CIF | emd_31220_validation.cif.gz | 6.4 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-31220 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-31220 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_31220.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.07 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : Mammalian orthoreovirus 3 Dearing
Entire | Name: Mammalian orthoreovirus 3 Dearing |
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Components |
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-Supramolecule #1: Mammalian orthoreovirus 3 Dearing
Supramolecule | Name: Mammalian orthoreovirus 3 Dearing / type: virus / ID: 1 / Parent: 0 / NCBI-ID: 10886 / Sci species name: Mammalian orthoreovirus 3 Dearing / Virus type: VIRUS-LIKE PARTICLE / Virus isolate: SEROTYPE / Virus enveloped: No / Virus empty: No |
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-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 56.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 21420 |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |