+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-30108 | ||||||||||||
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タイトル | Cryo-Em structure of eukaryotic pre-60S ribosome subunit from Saccharomyces cerevisiae rpf2 delta 255-344 strain, C4 state. | ||||||||||||
マップデータ | Cryo-EM structure of pre-60S ribosomal subunit from Saccharomyces cerevisiae rpf2 delta 255-344 strain, C4 state | ||||||||||||
試料 |
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キーワード | pre-60s / Rpf2 / RIBOSOME | ||||||||||||
機能・相同性 | 機能・相同性情報 protein-RNA complex remodeling / regulation of ribosomal subunit export from nucleus / exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear exosome (RNase complex) / 加水分解酵素 / PeBoW complex / 7S RNA binding / rRNA primary transcript binding / positive regulation of ATP-dependent activity / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ...protein-RNA complex remodeling / regulation of ribosomal subunit export from nucleus / exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear exosome (RNase complex) / 加水分解酵素 / PeBoW complex / 7S RNA binding / rRNA primary transcript binding / positive regulation of ATP-dependent activity / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of 5.8S rRNA / hexon binding / pre-mRNA 5'-splice site binding / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / proteasome binding / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / ribosomal large subunit binding / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / negative regulation of mRNA splicing, via spliceosome / preribosome, large subunit precursor / nuclear-transcribed mRNA catabolic process / ATPase activator activity / L13a-mediated translational silencing of Ceruloplasmin expression / translational elongation / ribosomal large subunit export from nucleus / regulation of translational fidelity / protein-RNA complex assembly / ribonucleoprotein complex binding / ribosomal subunit export from nucleus / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / Neutrophil degranulation / translation initiation factor activity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / proteasome complex / nuclear periphery / assembly of large subunit precursor of preribosome / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / cytosolic ribosome assembly / small-subunit processome / macroautophagy / maintenance of translational fidelity / protein catabolic process / rRNA processing / metallopeptidase activity / protein transport / ribosome biogenesis / viral capsid / protein-macromolecule adaptor activity / ATPase binding / 5S rRNA binding / large ribosomal subunit rRNA binding / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / nucleic acid binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / GTPase activity / mRNA binding / host cell nucleus / GTP binding / nucleolus / proteolysis / RNA binding / zinc ion binding / nucleoplasm / identical protein binding / nucleus / metal ion binding / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||||||||
生物種 | Saccharomyces cerevisiae S288C (パン酵母) / Saccharomyces cerevisiae S288c (パン酵母) | ||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.2 Å | ||||||||||||
データ登録者 | Li Y / Micic J | ||||||||||||
資金援助 | 中国, 3件
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引用 | ジャーナル: Nat Commun / 年: 2020 タイトル: Coupling of 5S RNP rotation with maturation of functional centers during large ribosomal subunit assembly. 著者: Jelena Micic / Yu Li / Shan Wu / Daniel Wilson / Beril Tutuncuoglu / Ning Gao / John L Woolford / 要旨: The protein composition and structure of assembling 60S ribosomal subunits undergo numerous changes as pre-ribosomes transition from the nucleolus to the nucleoplasm. This includes stable anchoring ...The protein composition and structure of assembling 60S ribosomal subunits undergo numerous changes as pre-ribosomes transition from the nucleolus to the nucleoplasm. This includes stable anchoring of the Rpf2 subcomplex containing 5S rRNA, rpL5, rpL11, Rpf2 and Rrs1, which initially docks onto the flexible domain V of rRNA at earlier stages of assembly. In this work, we tested the function of the C-terminal domain (CTD) of Rpf2 during these anchoring steps, by truncating this extension and assaying effects on middle stages of subunit maturation. The rpf2Δ255-344 mutation affects proper folding of rRNA helices H68-70 during anchoring of the Rpf2 subcomplex. In addition, several assembly factors (AFs) are absent from pre-ribosomes or in altered conformations. Consequently, major remodeling events fail to occur: rotation of the 5S RNP, maturation of the peptidyl transferase center (PTC) and the nascent polypeptide exit tunnel (NPET), and export of assembling subunits to the cytoplasm. | ||||||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_30108.map.gz | 192.9 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-30108-v30.xml emd-30108.xml | 74.8 KB 74.8 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_30108.png | 69.5 KB | ||
Filedesc metadata | emd-30108.cif.gz | 17.1 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-30108 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-30108 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_30108_validation.pdf.gz | 624.2 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_30108_full_validation.pdf.gz | 623.8 KB | 表示 | |
XML形式データ | emd_30108_validation.xml.gz | 7.1 KB | 表示 | |
CIF形式データ | emd_30108_validation.cif.gz | 8.2 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-30108 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-30108 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_30108.map.gz / 形式: CCP4 / 大きさ: 244.1 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Cryo-EM structure of pre-60S ribosomal subunit from Saccharomyces cerevisiae rpf2 delta 255-344 strain, C4 state | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.057 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
+全体 : Eukaryotic pre-60S ribosomal subunits
+超分子 #1: Eukaryotic pre-60S ribosomal subunits
+分子 #1: RDN25-1 rRNA
+分子 #2: RDN58-1 rRNA
+分子 #3: RDN5-2 rRNA
+分子 #6: ITS2-1 miscRNA
+分子 #4: Probable metalloprotease ARX1
+分子 #5: rRNA-processing protein CGR1
+分子 #7: 60S ribosomal protein L2-A
+分子 #8: 60S ribosomal protein L3
+分子 #9: 60S ribosomal protein L4-A
+分子 #10: 60S ribosomal protein L5
+分子 #11: 60S ribosomal protein L6-A
+分子 #12: 60S ribosomal protein L7-A
+分子 #13: 60S ribosomal protein L8-A
+分子 #14: 60S ribosomal protein L9-A
+分子 #15: Bud site selection protein 20
+分子 #16: 60S ribosomal protein L11-A
+分子 #17: Proteasome-interacting protein CIC1
+分子 #18: 60S ribosomal protein L13-A
+分子 #19: 60S ribosomal protein L14-A
+分子 #20: 60S ribosomal protein L15-A
+分子 #21: 60S ribosomal protein L16-A
+分子 #22: 60S ribosomal protein L17-A
+分子 #23: 60S ribosomal protein L18-A
+分子 #24: 60S ribosomal protein L19-A
+分子 #25: 60S ribosomal protein L20-A
+分子 #26: 60S ribosomal protein L21-A
+分子 #27: 60S ribosomal protein L22-A
+分子 #28: 60S ribosomal protein L23-A
+分子 #29: Ribosome assembly factor MRT4
+分子 #30: 60S ribosomal protein L25
+分子 #31: 60S ribosomal protein L26-A
+分子 #32: 60S ribosomal protein L27-A
+分子 #33: 60S ribosomal protein L28
+分子 #34: Nucleolar GTP-binding protein 1
+分子 #35: 60S ribosomal protein L30
+分子 #36: 60S ribosomal protein L31-A
+分子 #37: 60S ribosomal protein L32
+分子 #38: 60S ribosomal protein L33-A
+分子 #39: 60S ribosomal protein L34-A
+分子 #40: 60S ribosomal protein L35-A
+分子 #41: 60S ribosomal protein L36-A
+分子 #42: 60S ribosomal protein L37-A
+分子 #43: 60S ribosomal protein L38
+分子 #44: 60S ribosomal protein L39
+分子 #45: Nucleolar GTP-binding protein 2
+分子 #46: Pescadillo homolog
+分子 #47: Ribosome biogenesis protein 15
+分子 #48: 60S ribosomal protein L43-A
+分子 #49: Ribosome biogenesis protein NOP53
+分子 #50: Ribosome biogenesis protein NSA2
+分子 #51: Nuclear GTP-binding protein NUG1
+分子 #52: Ribosome biogenesis protein RLP7
+分子 #53: Ribosome biogenesis protein RLP24
+分子 #54: Ribosome biogenesis protein RPF2
+分子 #55: Regulator of ribosome biosynthesis
+分子 #56: Ribosome assembly protein 4
+分子 #57: Eukaryotic translation initiation factor 6
+分子 #58: UPF0642 protein YBL028C
+分子 #59: ZINC ION
+分子 #60: GUANOSINE-5'-TRIPHOSPHATE
+分子 #61: MAGNESIUM ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 1.9 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: OTHER |
電子光学系 | 照射モード: SPOT SCAN / 撮影モード: OTHER |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
初期モデル | モデルのタイプ: EMDB MAP |
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最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.2 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 53177 |
初期 角度割当 | タイプ: ANGULAR RECONSTITUTION |
最終 角度割当 | タイプ: ANGULAR RECONSTITUTION |