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- EMDB-29397: Structure of Mycobacterium smegmatis Rsh bound to a 70S translati... -

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Basic information

Entry
Database: EMDB / ID: EMD-29397
TitleStructure of Mycobacterium smegmatis Rsh bound to a 70S translation initiation complex
Map dataM. smegmatis 70S-Rsh-fMet-tRNA complex
Sample
  • Complex: Mycobacterium smegmatis 70S ribosome bound to Rsh, and initiator tRNA-fMet
    • Protein or peptide: x 53 types
    • RNA: x 5 types
Keywordsstarvation sensing / RelA/SpoT homologue / Mycobacterium / 70S initiation complex / TRANSLATION
Function / homology
Function and homology information


GTP diphosphokinase activity / guanosine tetraphosphate biosynthetic process / GTP diphosphokinase / large ribosomal subunit rRNA binding / large ribosomal subunit / small ribosomal subunit / 5S rRNA binding / kinase activity / transferase activity / tRNA binding ...GTP diphosphokinase activity / guanosine tetraphosphate biosynthetic process / GTP diphosphokinase / large ribosomal subunit rRNA binding / large ribosomal subunit / small ribosomal subunit / 5S rRNA binding / kinase activity / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / phosphorylation / mRNA binding / cytoplasm
Similarity search - Function
RelA/SpoT, AH and RIS domains / RelA/SpoT, AH and RIS domains / HD domain / RelA/SpoT family / RelA/SpoT, TGS domain / ACT domain / Region found in RelA / SpoT proteins / RelA/SpoT / Region found in RelA / SpoT proteins / TGS domain ...RelA/SpoT, AH and RIS domains / RelA/SpoT, AH and RIS domains / HD domain / RelA/SpoT family / RelA/SpoT, TGS domain / ACT domain / Region found in RelA / SpoT proteins / RelA/SpoT / Region found in RelA / SpoT proteins / TGS domain / ACT domain profile. / ACT domain / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / ACT-like domain / HD domain profile. / TGS domain profile. / TGS / TGS-like / HD domain / Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein L10 / : / Beta-grasp domain superfamily / Metal dependent phosphohydrolases with conserved 'HD' motif. / HD/PDEase domain / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L11, bacterial-type / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L11, conserved site / Ribosomal protein L10-like domain superfamily / Nucleotidyltransferase superfamily / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein L11 signature. / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L11, N-terminal / Ribosomal protein L17 signature. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11, RNA binding domain / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S19, bacterial-type
Similarity search - Domain/homology
AURKAIP1/COX24 domain-containing protein / Small ribosomal subunit protein bS6 / 30S ribosomal protein S10 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL10 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 ...AURKAIP1/COX24 domain-containing protein / Small ribosomal subunit protein bS6 / 30S ribosomal protein S10 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL10 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Uncharacterized protein / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL25 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein bS18A / Small ribosomal subunit protein uS14A / Large ribosomal subunit protein bL33B / 50S ribosomal protein L28 / 50S ribosomal protein L31 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL34 / GTP pyrophosphokinase RelA / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL15
Similarity search - Component
Biological speciesMycolicibacterium smegmatis MC2 155 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.76 Å
AuthorsMajumdar S / Sharma MR / Manjari SR / Banavali NK / Agrawal RK
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI132422 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM61576 United States
CitationJournal: Proc Natl Acad Sci U S A / Year: 2023
Title: Starvation sensing by mycobacterial RelA/SpoT homologue through constitutive surveillance of translation.
Authors: Yunlong Li / Soneya Majumdar / Ryan Treen / Manjuli R Sharma / Jamie Corro / Howard B Gamper / Swati R Manjari / Jerome Prusa / Nilesh K Banavali / Christina L Stallings / Ya-Ming Hou / ...Authors: Yunlong Li / Soneya Majumdar / Ryan Treen / Manjuli R Sharma / Jamie Corro / Howard B Gamper / Swati R Manjari / Jerome Prusa / Nilesh K Banavali / Christina L Stallings / Ya-Ming Hou / Rajendra K Agrawal / Anil K Ojha /
Abstract: The stringent response, which leads to persistence of nutrient-starved mycobacteria, is induced by activation of the RelA/SpoT homolog (Rsh) upon entry of a deacylated-tRNA in a translating ribosome. ...The stringent response, which leads to persistence of nutrient-starved mycobacteria, is induced by activation of the RelA/SpoT homolog (Rsh) upon entry of a deacylated-tRNA in a translating ribosome. However, the mechanism by which Rsh identifies such ribosomes in vivo remains unclear. Here, we show that conditions inducing ribosome hibernation result in loss of intracellular Rsh in a Clp protease-dependent manner. This loss is also observed in nonstarved cells using mutations in Rsh that block its interaction with the ribosome, indicating that Rsh association with the ribosome is important for Rsh stability. The cryo-EM structure of the Rsh-bound 70S ribosome in a translation initiation complex reveals unknown interactions between the ACT domain of Rsh and components of the ribosomal L7/L12 stalk base, suggesting that the aminoacylation status of A-site tRNA is surveilled during the first cycle of elongation. Altogether, we propose a surveillance model of Rsh activation that originates from its constitutive interaction with the ribosomes entering the translation cycle.
History
DepositionJan 6, 2023-
Header (metadata) releaseMay 17, 2023-
Map releaseMay 17, 2023-
UpdateJun 28, 2023-
Current statusJun 28, 2023Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_29397.png

Downloads & links

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Map

FileDownload / File: emd_29397.map.gz / Format: CCP4 / Size: 476.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationM. smegmatis 70S-Rsh-fMet-tRNA complex
Voxel sizeX=Y=Z: 0.84744 Å
Density
Contour LevelBy AUTHOR: 0.003
Minimum - Maximum-0.010060507 - 0.040820464
Average (Standard dev.)0.00013563005 (±0.0018278987)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions500500500
Spacing500500500
CellA=B=C: 423.72 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: M. smegmatis 70S-Rsh-fMet-tRNA complex- Composite Map

Fileemd_29397_additional_1.map
AnnotationM. smegmatis 70S-Rsh-fMet-tRNA complex- Composite Map
Projections & Slices
AxesZYX

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Slices (1/2)
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Histogram (log scale)

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Half map: M. smegmatis 70S-Rsh-fMet-tRNA complex half map 1

Fileemd_29397_half_map_1.map
AnnotationM. smegmatis 70S-Rsh-fMet-tRNA complex half map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: M. smegmatis 70S-Rsh-fMet-tRNA complex half map 1

Fileemd_29397_half_map_2.map
AnnotationM. smegmatis 70S-Rsh-fMet-tRNA complex half map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Sample components

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Entire : Mycobacterium smegmatis 70S ribosome bound to Rsh, and initiator ...

EntireName: Mycobacterium smegmatis 70S ribosome bound to Rsh, and initiator tRNA-fMet
Components
  • Complex: Mycobacterium smegmatis 70S ribosome bound to Rsh, and initiator tRNA-fMet
    • Protein or peptide: 50S ribosomal protein L33 2Ribosome
    • Protein or peptide: 50S ribosomal protein L28
    • RNA: fMet tRNA (77-MER)
    • Protein or peptide: 30S ribosomal protein S19
    • RNA: 5S rRNA (118-MER)
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein eL31
    • RNA: 23S rRNA (3119-MER)
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L9
    • Protein or peptide: 50S ribosomal protein L10
    • Protein or peptide: 50S ribosomal protein L11
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • RNA: 16S rRNA (1511-MER)
    • Protein or peptide: 30S ribosomal protein S22
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S20
    • Protein or peptide: 30S ribosomal protein S2
    • RNA: mRNAMessenger RNA
    • Protein or peptide: GTP pyrophosphokinase RelA
    • Protein or peptide: 50S ribosomal protein L31
    • Protein or peptide: 30S ribosomal protein S18 1Ribosome
    • Protein or peptide: 30S ribosomal protein S14

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Supramolecule #1: Mycobacterium smegmatis 70S ribosome bound to Rsh, and initiator ...

SupramoleculeName: Mycobacterium smegmatis 70S ribosome bound to Rsh, and initiator tRNA-fMet
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#58

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Macromolecule #1: 50S ribosomal protein L33 2

MacromoleculeName: 50S ribosomal protein L33 2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 6.484445 KDa
SequenceString:
ARNEIRPIVK LRSTAGTGYT YVTRKNRRND PDRIVLRKYD PVLRRHVEFR EER

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Macromolecule #2: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 8.729995 KDa
SequenceString:
SAHCQVTGRG PGFGNSVSHS HRRTPRRWDP NIQNKTYYLP SEGRRIRLRV SAKGIKVIDR DGIEAVVARM RARGEKV

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Macromolecule #4: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 9.379874 KDa
SequenceString:
PRSLKKGPFV DDHLLKKVDV QNEKNTKQVI KTWSRRSTII PDFIGHTFAV HDGRKHVPVF VTEAMVGHKL GEFAPTRTFK GH

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Macromolecule #6: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.374481 KDa
SequenceString:
ARLVGVDLPR DKRMEIALTY IYGIGRTRSN EILAATGIDK NMRTKDLTDD QVTVLRDYIE GNLKVEGDLR REVQADIRRK IEIGCYQGL RHRRGLPVRG QRTKTNARTR KGPKRTI

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Macromolecule #7: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 15.498937 KDa
SequenceString:
MLIPRKVKHR KQHHPEQRGI ASGGTSVSFG DYGIQALEHA YITNRQIESA RIAINRHIKR GGKVWINIFP DRPLTKKPAE TRMGSGKGS PEWWVANVKP GRVLFELSYP DEKTARDALT RAIHKLPIKA RIVTREE

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Macromolecule #8: 50S ribosomal protein eL31

MacromoleculeName: 50S ribosomal protein eL31 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 2.710179 KDa
SequenceString:
AKRGRKKRDR KHSKANHGKR PNA

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Macromolecule #10: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 30.008379 KDa
SequenceString: GIRKYKPTTP GRRGASVSDF AEITRSTPEK SLVRPLHGKG GRNAHGRITT RHKGGGHKRA YRVIDFRRHD KDGVNAKVAH IEYDPNRTA NIALLHYLDG EKRYIIAPQG LKQGDVIESG ANADIKPGNN LPLRNIPAGT VIHAVELRPG GGAKLARSAG V SIQLLGKE ...String:
GIRKYKPTTP GRRGASVSDF AEITRSTPEK SLVRPLHGKG GRNAHGRITT RHKGGGHKRA YRVIDFRRHD KDGVNAKVAH IEYDPNRTA NIALLHYLDG EKRYIIAPQG LKQGDVIESG ANADIKPGNN LPLRNIPAGT VIHAVELRPG GGAKLARSAG V SIQLLGKE GTYAALRMPS GEIRRVDVRC RATVGEVGNA EQSNINWGKA GRMRWKGKRP TVRGVVMNPV DHPHGGGEGK TS GGRHPVS PWGKPEGRTR KPNKPSDKLI VRRRRTGK

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Macromolecule #11: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 22.621902 KDa
SequenceString: ARKGILGTKL GMTQVFDENN KVVPVTVVKA GPNVVTRIRT TERDGYSAVQ LAYGEISPRK VIKPVAGQFA AAGVNPRRHV AELRLDDEA AVAEYEVGQE LTAEIFSDGA YVDVTGTSKG KGFAGTMKRH GFRGQGAAHG AQAVHRRPGS IGGCATPGRV F KGTRMSGR ...String:
ARKGILGTKL GMTQVFDENN KVVPVTVVKA GPNVVTRIRT TERDGYSAVQ LAYGEISPRK VIKPVAGQFA AAGVNPRRHV AELRLDDEA AVAEYEVGQE LTAEIFSDGA YVDVTGTSKG KGFAGTMKRH GFRGQGAAHG AQAVHRRPGS IGGCATPGRV F KGTRMSGR MGNDRVTTQN LKVHKVDAEN GVLLIKGAIP GRNGGLVVVR SAIKRG

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Macromolecule #12: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 22.346301 KDa
SequenceString: TLKVDVKTPA GKTDGSVELP AELFDVEPNI ALMHQVVTAQ LAAKRQGTHS TKTRGEVSGG GKKPYRQKGT GRARQGSTRA PQFTGGGTV HGPKPRDYSQ RTPKKMIAAA LRGALSDRAR NDRIHAVTEL VEGQTPSTKS AKTFLGTLTE NKKVLVVIGR T DEVGAKSV ...String:
TLKVDVKTPA GKTDGSVELP AELFDVEPNI ALMHQVVTAQ LAAKRQGTHS TKTRGEVSGG GKKPYRQKGT GRARQGSTRA PQFTGGGTV HGPKPRDYSQ RTPKKMIAAA LRGALSDRAR NDRIHAVTEL VEGQTPSTKS AKTFLGTLTE NKKVLVVIGR T DEVGAKSV RNLPGVHVIS PDQLNTYDVL NADDVVFSVE ALNAYISANS K

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Macromolecule #13: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 20.588701 KDa
SequenceString:
KALPRLKQRY REEIREALQQ EFNYANVMQI PGVVKVVVNM GVGDAARDAK LINGAINDLA LITGQKPEVR RARKSIAQFK LREGMPIGA RVTLRGDRMW EFLDRLISIA LPRIRDFRGL SPKQFDGTGN YTFGLNEQSM FHEIDVDSID RPRGMDITVV T TATNDAEG RALLRALGFP FKEN

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Macromolecule #14: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 19.165934 KDa
SequenceString:
SRIGKQPVPV PSGVDVTING QNLSVKGPKG TLTLDVAEPI SVSRAEDGAI VVTRPDDERR SRSLHGLSRT LIANLVTGVT EGYTQKMEI FGVGYRVQLK GQNLEFALGY SHPVLIEAPE GITFAVESPT KFSVSGIDKQ KVGQISAVIR RLRRPDPYKG K GVRYEGEQ IRRKVGKT

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Macromolecule #15: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 15.949253 KDa
SequenceString:
MKLILTAEVE HLGAAGDTVE VKDGYGRNYL LPRGLAIVAS RGAERQAEEI RRARESKVIR DIEHANELKT ALEGLGDVTL SVNAAGDTG KLFGSVTAAD VVNAIKKAGG PNLDKRTVQL AKAHIKSVGT HPVTVKLHTG VEAKVSLNVV AQ

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Macromolecule #16: 50S ribosomal protein L10

MacromoleculeName: 50S ribosomal protein L10 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.095021 KDa
SequenceString:
MAKADKATAV ADIAEQFKAS TATVVTEYRG LTVANLAELR RALGDSATYT VAKNTLVKRA ASEAGIEGLD ELFAGPTAIA FVKGEAVDA AKAIKKFAKD NKALVIKGGY MDGKALSVAD VEKIADL

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Macromolecule #17: 50S ribosomal protein L11

MacromoleculeName: 50S ribosomal protein L11 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 14.109185 KDa
SequenceString:
LIKLQIQAGQ ANPAPPVGPA LGQHGVNIME FCKAYNAATE SQRGNVIPVE ITVYEDRSFT FALKTPPAAK LLLKAAGVQK GSGEPHKTK VAKVTWDQVR EIAETKKADL NANDIDAAAK IIAGTARSMG ITVE

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Macromolecule #18: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 16.015493 KDa
SequenceString:
PTYTPKAGDT TRSWYVIDAS DVVLGRLASA AATLLRGKHK PTFTPNVDGG DFVIVINADK IAVSGDKLTK KFAYRHSGYP GGLRKRTIG ELLEKHPTRV VENAIIGMLP HNKLGRQIQK KLKVYAGPDH PHAAQQPIPF EIKQVAQ

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Macromolecule #19: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.400625 KDa
SequenceString:
MIQQESRLKV ADNTGAKEIL CIRVLGGSSR RYAGIGDVIV ATVKDAIPGG NVKRGDVVKA VVVRTVKERR RADGSYIKFD ENAAVIIKN DNDPRGTRIF GPVGRELREK KFMKIVSLAP EVL

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Macromolecule #20: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 15.383968 KDa
SequenceString:
VIKLHDLKPA PGEKKAKTRV GRGEGSKGKT AGRGTKGTKA RKNVPVMFEG GQMPIHMRLP KLKGFKNRFR TEYQVVNVGD INKAFPQGG TVGVDELVAK GLVRKNSLVK VLGDGKLTVK VDVTANKFSG SAREAITAAG GSATEL

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Macromolecule #21: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.210346 KDa
SequenceString:
PKPTKGPRLG GSSSHQSALL ANLATSLFEH GRIKTTEPKA RALRPYAEKL ITHAKKGALH NRREVMKKIR DKDVVHTLFA EIGPFYADR NGGYTRIIKV ENRKGDNAPM AVIELVREK

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Macromolecule #22: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.580531 KDa
SequenceString:
AHKPVGQNIS EVRRNARLRR HARLRKKVAG TAEVPRLVVN RSARHIHVQL VNDLNGTTLA AASSIEADVR AIDGDKKAHS VRVGQLIAE RAKAAGVETV VFDRGGYTYG GRIAALADAA REAGLKF

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Macromolecule #23: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 12.892806 KDa
SequenceString:
MNTLDFVDQA SLRDDIPTFS PGDTVNVHVK VIEGSKERIQ VFKGVVIRRQ GGGISETFTV RKESYGVGVE RTFPVHSPNI DHIDVLTRG DVRRAKLYYL RELRGKKAKI KEKR

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Macromolecule #24: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 14.035175 KDa
SequenceString:
ARVKRALNAQ KKRRTVLKAS KGYRGQRSRL YRKAKEQQLH SLTYAYRDRR ARKGEFRKLW ISRINAAARA NDITYNRLIQ GLKAAGVEV DRKNLAELAV SDPAAFTALV DVARAALPED VNAPS

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Macromolecule #25: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 10.72444 KDa
SequenceString:
TYAIVKTGGK QYKVAAGDVV KVEKLDSEPG ASVSLPVALV VDGANVTSKA DDLAKVAVTA EVLEHTKGPK IRIHKFKNKT GYHKRQGHR QQLTVLKVTG I

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Macromolecule #26: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 12.408192 KDa
SequenceString:
EFPSATAKAR YVRVSATKAR RVIDLVRGKS VEEALDILRW APQAASEPVA KVIASAAANA QNNEGLDPST LVVATVYADE GPTAKRIRP RAQGRAFRIR KRTSHITVIV ESRPP

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Macromolecule #27: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 10.748448 KDa
SequenceString:
ATITDPRDII LAPVISEKSY GLIEDNVYTF VVHPDSNKTQ IKIAIEKIFD VKVDSVNTAN RQGKRKRTRT GFGKRKSTKR AIVKLAAGS KPIDLFGA

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Macromolecule #28: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 11.228946 KDa
SequenceString:
MKVHKGDTVL VISGKDKGAK GKVLVAYPDR NKVLVEGVNR IKKHTAVSAN ERGASSGGIV TQEAPIHVSN VMVVDSDGKP TRVGYRIDD ETGKKVRIAK TNGKDI

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Macromolecule #29: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 20.331725 KDa
SequenceString: TANIPNKLTA NVRTRTGKGA SRQARRDGKV PAVLYGHGTD PQHLELNARD FAAVLRSHGT NAILTLDIEG TEQLALTKAL DVHPIRRNI QHADLLVVQR GEKVTVEVTV LVEGDATPGT LVTQDANTIE IEAEALSIPE QLTVSVEGVE AGTQITAGQI S LPEGVNLI ...String:
TANIPNKLTA NVRTRTGKGA SRQARRDGKV PAVLYGHGTD PQHLELNARD FAAVLRSHGT NAILTLDIEG TEQLALTKAL DVHPIRRNI QHADLLVVQR GEKVTVEVTV LVEGDATPGT LVTQDANTIE IEAEALSIPE QLTVSVEGVE AGTQITAGQI S LPEGVNLI SDPELLVVNV VEAPSAEALE EEGA

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Macromolecule #30: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 8.322479 KDa
SequenceString:
SSSRNGRDSA AQRLGVKRFG GQVVKAGEIL VRQRGTHFHP GVNVGRGGDD TLFALAPGAV EFGAKRGRKT VNIVPVARP

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Macromolecule #31: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 7.590576 KDa
SequenceString:
GTTPGELREL TDDELKDKLR ESKEELFNLR FQMATGQLSN NRRLRTVRQE IARVYTVLRE RELG

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Macromolecule #32: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 6.761772 KDa
SequenceString:
AELKITQVRS TIGARWKQRE SLRTLGLKKI RQSVVREDNA QTRGLINTVH HLVEVEEVG

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Macromolecule #33: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 6.050149 KDa
SequenceString:
AVPKRRMSRA NTRSRRAQWK AEAPGLVTVS VAGQQRKVPR RLLKAARLGL VDLD

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Macromolecule #34: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 5.391384 KDa
SequenceString:
AKGKRTFQPN NRRRARVHGF RLRMRTRAGR AIVANRRSKG RRALTA

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Macromolecule #35: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 7.16731 KDa
SequenceString:
PKAKTHSGAS KRFRRTGTGK IVRQKANRRH LLEHKPTKRT RRLDGRTTVS AADNSRINKL LNG

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Macromolecule #36: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 4.341277 KDa
SequenceString:
MKVNPSVKPI CDKCRVIRRH GRVMVICSDP RHKQRQG

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Macromolecule #38: 30S ribosomal protein S22

MacromoleculeName: 30S ribosomal protein S22 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 4.033104 KDa
SequenceString:
GSVIKKRRKR MSKKKHRKLL RRTRVQRRKL GK

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Macromolecule #39: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 23.61715 KDa
SequenceString: GQKINPHGFR LGITTEWKSR WYADKQYKDY VKEDVAIRKL LATGLERAGI ADVEIERTRD RVRVDIHTAR PGIVIGRRGT EADRIRADL EKLTGKQVQL NILEVKNPES QAQLVAQGVA EQLSNRVAFR RAMRKAIQSA MRQPNVKGIR VQCSGRLGGA E MSRSEFYR ...String:
GQKINPHGFR LGITTEWKSR WYADKQYKDY VKEDVAIRKL LATGLERAGI ADVEIERTRD RVRVDIHTAR PGIVIGRRGT EADRIRADL EKLTGKQVQL NILEVKNPES QAQLVAQGVA EQLSNRVAFR RAMRKAIQSA MRQPNVKGIR VQCSGRLGGA E MSRSEFYR EGRVPLHTLR ADIDYGLYEA KTTFGRIGVK VWIYKGDIVG

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Macromolecule #40: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 23.28459 KDa
SequenceString: ARYTGPATRK SRRLGVDLVG GDQSFEKRPY PPGQHGRARI KESEYRQQLQ EKQKARFSYG VMEKQFRRYY EEANRQPGKT GDNLLRILE SRLDNVVYRA GLARTRRMAR QLVSHGHFLV NGVKVDIPSY RVSQYDIIDV KEKSLNTLPF QIARETAGER P IPSWLQVV ...String:
ARYTGPATRK SRRLGVDLVG GDQSFEKRPY PPGQHGRARI KESEYRQQLQ EKQKARFSYG VMEKQFRRYY EEANRQPGKT GDNLLRILE SRLDNVVYRA GLARTRRMAR QLVSHGHFLV NGVKVDIPSY RVSQYDIIDV KEKSLNTLPF QIARETAGER P IPSWLQVV GERQRILVHQ LPERAQIDVP LTEQLIVELY SK

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Macromolecule #41: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 18.481439 KDa
SequenceString:
NYIERVVSIN RVSKVVKGGR RFSFTALVIV GDGKGMVGVG YGKAKEVPAA IAKGVEEARK NFFRVPLIGS TITHPVQGEA AAGVVMLRP ASPGTGVIAG GAARAVLECA GVHDILAKSL GSDNAINVVH ATVAALKLLQ RPEEVAARRG LPIEDVAPAG M LKARRESE ALAAAAAREG SA

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Macromolecule #42: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 10.991637 KDa
SequenceString:
MRPYEIMVIL DPTLDERTVA PSLETFLNVI RKDGGTVDKV DIWGRRRLAY EIAKHAEGIY AVIDVKAEPA TVSELDRQLN LNESVLRTK VLRTDKH

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Macromolecule #43: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 17.52918 KDa
SequenceString:
PRKGPAPKRP LVNDPVYGSQ LVTQLVNKVL LEGKKSLAER IVYGALEQAR EKTGTDPVVT LKRALDNVKP ALEVRSRRVG GATYQVPVE VRPDRSTTLA LRWLVNFSRQ RREKTMVERL ANEILDASNG LGASVKRRED THKMAEANRA FAHYRW

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Macromolecule #44: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 14.361442 KDa
SequenceString:
TMTDPIADFL TRLRNANSAY HDEVTLPHSK LKANIAEILK REGYISDYRT EDARVGKSLV VQLKYGPSRE RSIAGLRRVS KPGLRVYAK STNLPRVLGG LGVAIISTSS GLLTDRQAAR QGVGGEVLAY VW

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Macromolecule #45: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 14.093392 KDa
SequenceString:
PIQTVGRRKE AVVRVRLVPG TGQFNLDGRT LENYFPNKVH QQLIKAPLVT VDRVDQFDIY AHLDGGGPSG QAGALRLAIA RALILVQPE DRPALKKAGF LTRDPRAIER KKYGLKKARK APQYSKR

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Macromolecule #46: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 11.252039 KDa
SequenceString:
GQKIRIRLKA YDHEAIDASA RKIVETVTRT GASVVGPVPL PTEKNVYCVI RSPHKYKDSR EHFEMRTHKR LIDILDPTPK TVDALMRID LPASVDVNIQ

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Macromolecule #47: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 12.151729 KDa
SequenceString:
KNVPHGAAHI KSTFNNTIVS ITDPQGNVIA WASSGHVGFK GSRKSTPFAA QLAAENAARK AQEHGVKKVD VFVKGPGSGR ETAIRSLQA AGLEVGTISD VTPQPHNGCR PPKRRR

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Macromolecule #48: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.678093 KDa
SequenceString:
PTIQQLVRKG RRDKIAKVKT AALKGSPQRR GVCTRVYTTT PKKPNSALRK VARVKLTSQV EVTAYIPGEG HNLQEHSMVL VRGGRVKDL PGVRYKIIRG SLDTQGVKNR KQARSRYGAK KEK

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Macromolecule #49: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 10.2369 KDa
SequenceString:
ALTAEQKKEI LGQYGLHDTD TGSPEAQVAL LTKRIQDLTE HLKVHKHDHH SRRGLLLLVG RRRRLLKYVA QVDVARYRSL IERLGLRR

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Macromolecule #50: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 12.635652 KDa
SequenceString:
AVKIKLTRLG KIRNPQYRII VADARTRRDG RAIEVIGRYH PKEEPSLIQI DSERAQYWLG VGAQPTEPVL ALLKITGDWQ KFKGLPGAE GTLKVKEPKP SKLDLFNAAL AEAE

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Macromolecule #51: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 10.680461 KDa
SequenceString:
QKGPKYTPAA EKPRGRRKTA IGYVVSDKMQ KTIVVELEDR KSHPLYGKII RTTKKVKAHD ENGEAGIGDR VSLMETRPLS ATKRWRLVE ILEKA

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Macromolecule #52: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 9.424908 KDa
SequenceString:
ANIKSQIKRI RTNERRRLRN QSVKSSLRTA IRGFREAVDA GDKDKASELL HATSRKLDKA ASKGVIHPNQ AANKKSALAL ALNKL

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Macromolecule #53: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 25.576547 KDa
SequenceString: MAVVTMKQLL DSGAHFGHQT RRWNPKMKRF IFTDRNGIYI IDLQQTLTYI DKAYEFVKET VAHGGTVLFV GTKKQAQESI AEEATRVGM PYVNQRWLGG MLTNFSTVHK RLQRLKELEA MEQTGGFEGR TKKEILMLTR EKNKLERSLG GIRDMQKVPS A VWVVDTNK ...String:
MAVVTMKQLL DSGAHFGHQT RRWNPKMKRF IFTDRNGIYI IDLQQTLTYI DKAYEFVKET VAHGGTVLFV GTKKQAQESI AEEATRVGM PYVNQRWLGG MLTNFSTVHK RLQRLKELEA MEQTGGFEGR TKKEILMLTR EKNKLERSLG GIRDMQKVPS A VWVVDTNK EHIAVGEARK LGIPVIAILD TNCDPDVVDY PIPGNDDAIR SAALLTKVIA SAVAEGLQAR

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Macromolecule #55: GTP pyrophosphokinase RelA

MacromoleculeName: GTP pyrophosphokinase RelA / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 88.057672 KDa
Recombinant expressionOrganism: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria)
SequenceString: MVDEPGKGQA VQSPPAVPET VPKPPSGTAP TTTPAAPSTS SETARISASR RVRARLARRM TAQRSAINPV LEPLVAVHRE IYPKADLQL LQRAYEVAEK RHADQLRKSG DPYITHPLAV ANILAELGMD TTTLVAALLH DTVEDTGYSL EALTADFGSE V GHLVDGVT ...String:
MVDEPGKGQA VQSPPAVPET VPKPPSGTAP TTTPAAPSTS SETARISASR RVRARLARRM TAQRSAINPV LEPLVAVHRE IYPKADLQL LQRAYEVAEK RHADQLRKSG DPYITHPLAV ANILAELGMD TTTLVAALLH DTVEDTGYSL EALTADFGSE V GHLVDGVT KLDKVVLGSA AEGETIRKMI IAMARDPRVL VIKVADRLHN MRTMRFLPPE KQARKARETL EVIAPLAHRL GM ATVKWEL EDLAFAILHP KKYEEIVRLV ADRAPSRDTY LAKVRAEIGV ALSAMKINAV VEGRPKHYWS IYQKMIVKGR DFD DIHDLV GVRILCDEIR DCYAAVGVVH SLWQPMAGRF KDYIAQPRYG VYQSLHTTVV GPEGKPLEVQ IRTREMHRTA EYGI AAHWR YKEAKGRNGV PHSHAAAEID DMAWMRQLLD WQREAADPGE FLESLRYDLA TQEIFVFTPK GDVITLPAGS TPVDF AYAV HTEVGHRCIG ARVNGRLVAL ERKLENGEVV EVFTSKAPNA GPSRDWQGFV VSPRAKTKIR QWFAKERREE ALESGK DAI AREVRRGGLP LQRLMNAETM GALARELRYQ DVSALYTAVG EGHVSARHVV QRLLAQFGGD DAAEDELAER STPATMP MR QRSNDDTGVA VPGAPGVLTK LAKCCTPVPG DTIMGFVTRG GGVSVHRTDC TNAESLQQQS ERIIEVNWAP SPSSVFLV A IQVEALDRHR LLSDVTRVLA DEKVNILSAS VTTSNDRVAI SRFTFEMGDP KHLGHLLSVV RNVEGVYDVY RVTSAA

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Macromolecule #56: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 9.141198 KDa
SequenceString:
MKPGIHPDYH PVVFQDAATG AQFLTRSTAT STRTIEWPTP SGPKTYPLIV VDVTSDSHPF WTGSARHIDS AGQVEKFRRR YG

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Macromolecule #57: 30S ribosomal protein S18 1

MacromoleculeName: 30S ribosomal protein S18 1 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 9.449175 KDa
SequenceString:
MMAVKKSRKR TAATELKKPR RNQLEALGVT TIDYKDVAVL RTFLSERGKI RSRHVTGLTP QQQRQVATAI KNAREMALLP MAGP

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Macromolecule #58: 30S ribosomal protein S14

MacromoleculeName: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 11.66153 KDa
SequenceString:
AKKSKIVKNE QRRELVQRYA ERRAELKRTI RDPASSPERR AAAVSALQRL PRDSSPVRLR NRDVVDGRPR GHLRKFGLSR VRVREMAHR GELPGVRKAS W

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Macromolecule #3: fMet tRNA (77-MER)

MacromoleculeName: fMet tRNA (77-MER) / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 24.78073 KDa
SequenceString:
CGCGGGGUGG AGCAGCUCGG UAGCUCGCUG GGCUCAUAAC CCAGAGGUCG CAGGUUCGAA UCCUGUCCCC GCUACCA

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Macromolecule #5: 5S rRNA (118-MER)

MacromoleculeName: 5S rRNA (118-MER) / type: rna / ID: 5 / Number of copies: 1
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 38.061816 KDa
SequenceString:
GUUACGGCGG UCCAUAGCGG CAGGGAAACG CCCGGUCCCA UCCCGAACCC GGAAGCUAAG CCUGCCAGCG CCGAUGAUAC UACCCAUCC GGGUGGAAAA GUAGGACACC GCCGAACAC

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Macromolecule #9: 23S rRNA (3119-MER)

MacromoleculeName: 23S rRNA (3119-MER) / type: rna / ID: 9 / Number of copies: 1
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 1.01183475 MDa
SequenceString: AAGUGUUUAA GGGCGCAUGG UGGAUGCCUU GGCACUGGGA GCCGAUGAAG GACGUAGGAG GCUGCGAUAA GCCUCGGGGA GCUGUCAAC CGAGCGUUGA UCCGAGGAUG UCCGAAUGGG GAAACCCGGC ACGAGUGAUG UCGUGUCACC AGGCGCUGAA U AUAUAGGC ...String:
AAGUGUUUAA GGGCGCAUGG UGGAUGCCUU GGCACUGGGA GCCGAUGAAG GACGUAGGAG GCUGCGAUAA GCCUCGGGGA GCUGUCAAC CGAGCGUUGA UCCGAGGAUG UCCGAAUGGG GAAACCCGGC ACGAGUGAUG UCGUGUCACC AGGCGCUGAA U AUAUAGGC GUCUGGGGGG AACGCGGGGA AGUGAAACAU CUCAGUACCC GUAGGAAGAG AAAACAAAAU GUGAUUCCGU GA GUAGUGG CGAGCGAAAG CGGAGGAUGG CUAAACCGUA UGCAUGUGAU ACCGGGUAGG GGUUGUGUGU GCGGGGUUGU GGG ACCUAU CUUUCCGGCU CUACCUGGCU GGAGGGCAGU GAGAAAAUGU UGUGGUUAGC GGAAAUGGCU UGGGAUGGCC UGCC GUAGA CGGUGAGAGC CCGGUACGUG AAAACCCGAC GUCUGUCUUG AUGGUGUUCC CGAGUAGCAG CGGGCCCGUG GAAUC UGCU GUGAAUCUGC CGGGACCACC CGGUAAGCCU GAAUACUUCC CAGUGACCGA UAGCGGAUUA GUACCGUGAG GGAAUG GUG AAAAGUACCC CGGGAGGGGA GUGAAAGAGU ACCUGAAACC GUGCGCUUAC AAUCCGUCAG AGCCCUCGAC GUGUCGU GG GGUGAUGGCG UGCCUUUUGA AGAAUGAGCC UGCGAGUCAG GGACAUGUCG CGAGGUUAAC CCGGGUGGGG UAGCCGCA G CGAAAGCGAG UCUGAAUAGG GCGUAUCCAC ACAAGAGUGU GUGGUGUAGU GGUGUGUUCU GGACCCGAAG CGGAGUGAU CUACCCAUGG CCAGGGUGAA GCGCGGGUAA GACCGCGUGG AGGCCCGAAC CCACUUAGGU UGAAGACUGA GGGGAUGAGC UGUGGGUAG GGGUGAAAGG CCAAUCAAAC UCCGUGAUAG CUGGUUCUCC CCGAAAUGCA UUUAGGUGCA GCGUCGCAUG U UUCUUGCC GGAGGUAGAG CUACUGGAUG GCCGAUGGGC CCCACAGGGU UACUGACGUC AGCCAAACUC CGAAUGCCGG UA AGUCCAA GAGUGCGGCA GUGAGACGGC GGGGGAUAAG CUCCGUGCGU CGAGAGGGAA ACAGCCCAGA UCGCCGGCUA AGG CCCCUA AGCGUGUGCU AAGUGGAAAA GGAUGUGCAG UCGCGAAGAC AACCAGGAGG UUGGCUUAGA AGCAGCCACC CUUG AAAGA GUGCGUAAUA GCUCACUGGU CAAGUGAUUG UGCGCCGAUA AUGUAGCGGG GCUCAAGCAC ACCGCCGAAG CCGCG GCAG CCAACGUGUU GGCUGGGUAG GGGAGCGUCC UGCAUCCGGU GAAGCCGCCG AGUGAUCGAG UGGUGGAGGG UGUGGG AGU GAGAAUGCAG GCAUGAGUAG CGAUUAGGCA AGUGAGAACC UUGCCCGCCG AAAGACCAAG GGUUCCUGGG CCAGGCC AG UCCGCCCAGG GUGAGUCGGG ACCUAAGGCG AGGCCGACAG GCGUAGUCGA UGGACAACGG GUUGAUAUUC CCGUACCC G UGUAUGUGCG UCCAUGAUGA AUCAGCGGUA CUAACCAUCC AAAACCACCG UGACCGCACC UUUCGGGGUG UGGCGUUGG UGGGGCUGCA UGGGACCUUC GUUGGUAGUA GUCAAGCGAU GGGGUGACGC AGGAAGGUAG CCGUACCGGU CAGUGGUAAU ACCGGGGUA AGCCUGUAGG GAGUCAGAUA GGUAAAUCCG UCUGGCAUAU AUCCUGAGAG GUGAUGCAUA GCCGAGUGAG G CGAAUUCG GUGAUCCUAU GCUGCCGAGA AAAGCCUCUA GCGAGGACAU ACACGGCCCG UACCCCAAAC CAACACAGGU GG UCAGGUA GAGAAUACUA AGGCGUACGA GUGAACUAUG GUUAAGGAAC UCGGCAAAAU GCCCCCGUAA CUUCGGGAGA AGG GGGACC CACAUGGCGU GUAAGCCUUU ACGGCCCAAG CGUGAGUGGG UGGCACAAAC CAGUGAGAAG CGACUGUUUA CUAA AAACA CAGGUCCGUG CGAAGUCGCA AGACGAUGUA UACGGACUGA CGCCUGCCCG GUGCUGGAAG GUUAAGAGGA CCCGU UAAC UCCCUUUGGG GGUGAAGCGG AGAAUUUAAG CCCCAGUAAA CGGCGGUGGU AACUAUAACC AUCCUAAGGU AGCGAA AUU CCUUGUCGGG UAAGUUCCGA CCUGCACGAA UGGCGUAACG ACUUCUCAAC UGUCUCAACC AUAGACUCGG CGAAAUU GC ACUACGAGUA AAGAUGCUCG UUACGCGCGG CAGGACGAAA AGACCCCGGG ACCUUCACUA CAACUUGGUA UUGGUGCU C GAUACGGUUU GUGUAGGAUA GGUGGGAGAC UGUGAAGCUC ACACGCCAGU GUGGGUGGAG UCGUUGUUGA AAUACCACU CUGAUCGUAU UGGGCCUCUA ACCUCGGACC GUAUAUCCGG UUCAGGGACA GUGCCUGGUG GGUAGUUUAA CUGGGGCGGU UGCCUCCUA AAAUGUAACG GAGGCGCCCA AAGGUUCCCU CAACCUGGAC GGCAAUCAGG UGUUGAGUGU AAGUGCACAA G GGAGCUUG ACUGCGAGAC GGACAUGUCG AGCAGGGACG AAAGUCGGGA CUAGUGAUCC GGCACCUCUG AGUGGAAGGG GU GUCGCUC AACGGAUAAA AGGUACCCCG GGGAUAACAG GCUGAUCUUC CCCAAGAGUC CAUAUCGACG GGAUGGUUUG GCA CCUCGA UGUCGGCUCG UCGCAUCCUG GGGCUGGAGC AGGUCCCAAG GGUUGGGCUG UUCGCCCAUU AAAGCGGCAC GCGA GCUGG GUUUAGAACG UCGUGAGACA GUUCGGUCUC UAUCCGCCGC GCGCGUCAGA AGCUUGAGGA AACCUGUCCC UAGUA CGAG AGGACCGGGA CGGACGAACC UCUGGUAUAC CAGUUGUCCC ACCAGGGGCA CGGCUGGAUA GCCACGUUCG GACAGG AUA ACCGCUGAAA GCAUCUAAGC GGGAAACCUC UUCCAAGACC AGGCUUCUCA CCCUCUAGGA GGGAUAAGGC CCCCCGC AG ACCACGGGAU UGAUAGACCA GACCUGGAAG CCUAGUAAUA GGUGCAGGGA ACUGGCACUA ACCGGCCGAA AACUUAC

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Macromolecule #37: 16S rRNA (1511-MER)

MacromoleculeName: 16S rRNA (1511-MER) / type: rna / ID: 37 / Number of copies: 1
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 490.111719 KDa
SequenceString: UUUGGAGAGU UUGAUCCUGG CUCAGGACGA ACGCUGGCGG CGUGCUUAAC ACAUGCAAGU CGAACGGAAA GGCCCUUUCG GGGGUACUC GAGUGGCGAA CGGGUGAGUA ACACGUGGGU GAUCUGCCCU GCACUUUGGG AUAAGCCUGG GAAACUGGGU C UAAUACCG ...String:
UUUGGAGAGU UUGAUCCUGG CUCAGGACGA ACGCUGGCGG CGUGCUUAAC ACAUGCAAGU CGAACGGAAA GGCCCUUUCG GGGGUACUC GAGUGGCGAA CGGGUGAGUA ACACGUGGGU GAUCUGCCCU GCACUUUGGG AUAAGCCUGG GAAACUGGGU C UAAUACCG AAUACACCCU GCUGGUCGCA UGGCCUGGUA GGGGAAAGCU UUUGCGGUGU GGGAUGGGCC CGCGGCCUAU CA GCUUGUU GGUGGGGUGA UGGCCUACCA AGGCGACGAC GGGUAGCCGG CCUGAGAGGG UGACCGGCCA CACUGGGACU GAG AUACGG CCCAGACUCC UACGGGAGGC AGCAGUGGGG AAUAUUGCAC AAUGGGCGCA AGCCUGAUGC AGCGACGCCG CGUG AGGGA UGACGGCCUU CGGGUUGUAA ACCUCUUUCA GCACAGACGA AGCGCAAGUG ACGGUAUGUG CAGAAGAAGG ACCGG CCAA CUACGUGCCA GCAGCCGCGG UAAUACGUAG GGUCCGAGCG UUGUCCGGAA UUACUGGGCG UAAAGAGCUC GUAGGU GGU UUGUCGCGUU GUUCGUGAAA ACUCACAGCU UAACUGUGGG CGUGCGGGCG AUACGGGCAG ACUAGAGUAC UGCAGGG GA GACUGGAAUU CCUGGUGUAG CGGUGGAAUG CGCAGAUAUC AGGAGGAACA CCGGUGGCGA AGGCGGGUCU CUGGGCAG U AACUGACGCU GAGGAGCGAA AGCGUGGGGA GCGAACAGGA UUAGAUACCC UGGUAGUCCA CGCCGUAAAC GGUGGGUAC UAGGUGUGGG UUUCCUUCCU UGGGAUCCGU GCCGUAGCUA ACGCAUUAAG UACCCCGCCU GGGGAGUACG GCCGCAAGGC UAAAACUCA AAGGAAUUGA CGGGGGCCCG CACAAGCGGC GGAGCAUGUG GAUUAAUUCG AUGCAACGCG AAGAACCUUA C CUGGGUUU GACAUGCACA GGACGCCGGC AGAGAUGUCG GUUCCCUUGU GGCCUGUGUG CAGGUGGUGC AUGGCUGUCG UC AGCUCGU GUCGUGAGAU GUUGGGUUAA GUCCCGCAAC GAGCGCAACC CUUGUCUCAU GUUGCCAGCA CGUUAUGGUG GGG ACUCGU GAGAGACUGC CGGGGUCAAC UCGGAGGAAG GUGGGGAUGA CGUCAAGUCA UCAUGCCCCU UAUGUCCAGG GCUU CACAC AUGCUACAAU GGCCGGUACA AAGGGCUGCG AUGCCGUGAG GUGGAGCGAA UCCUUUCAAA GCCGGUCUCA GUUCG GAUC GGGGUCUGCA ACUCGACCCC GUGAAGUCGG AGUCGCUAGU AAUCGCAGAU CAGCAACGCU GCGGUGAAUA CGUUCC CGG GCCUUGUACA CACCGCCCGU CACGUCAUGA AAGUCGGUAA CACCCGAAGC CGGUGGCCUA ACCCUUGUGG AGGGAGC CG UCGAAGGUGG GAUCGGCGAU UGGGACGAAG UCGUAACAAG GUAGCCGUAC CGGAAGGUGC GGCUGGAUC

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Macromolecule #54: mRNA

MacromoleculeName: mRNA / type: rna / ID: 54 / Number of copies: 1
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 6.502976 KDa
SequenceString:
AGGAGGUAAA AAUGUUCAAA

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
GridModel: Quantifoil / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 81000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 66.59 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1928609
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6dzi

Initial angle assignmentType: NOT APPLICABLE
Final 3D classificationSoftware - Name: RELION (ver. 4.0-beta-2)
Final angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 4.0-beta-2)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.76 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 4.0-beta-2) / Number images used: 36321
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-8fr8:
Structure of Mycobacterium smegmatis Rsh bound to a 70S translation initiation complex

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