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- EMDB-28981: Structure of the vertebrate augmin complex -

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Basic information

Entry
Database: EMDB / ID: EMD-28981
TitleStructure of the vertebrate augmin complex
Map dataSharpened map of X laevis augmin
Sample
  • Complex: Augmin
    • Protein or peptide: HAUS augmin-like complex subunit 1
    • Protein or peptide: HAUS augmin-like complex subunit 3
    • Protein or peptide: HAUS augmin like complex subunit 4 L homeolog
    • Protein or peptide: HAUS augmin-like complex subunit 5
    • Protein or peptide: HAUS augmin like complex subunit 2 L homeolog, Green fluorescent protein chimera
    • Protein or peptide: HAUS augmin like complex subunit 6 L homeolog
    • Protein or peptide: HAUS augmin like complex subunit 7 S homeolog
    • Protein or peptide: HAUS augmin-like complex subunit 8
Function / homology
Function and homology information


HAUS complex / microtubule minus-end binding / mitotic spindle microtubule / centrosome cycle / spindle assembly / bioluminescence / generation of precursor metabolites and energy / spindle pole / spindle / microtubule ...HAUS complex / microtubule minus-end binding / mitotic spindle microtubule / centrosome cycle / spindle assembly / bioluminescence / generation of precursor metabolites and energy / spindle pole / spindle / microtubule / cell division / centrosome / cytoplasm
Similarity search - Function
HAUS augmin-like complex subunit 2, metazoa / HAUS augmin-like complex subunit 6 / HAUS augmin-like complex subunit 6, N-terminal / HAUS augmin-like complex subunit 2 / HAUS augmin-like complex subunit 7-like / HAUS augmin-like complex subunit 6 N-terminus / HAUS augmin-like complex subunit 2 / HAUS augmin-like complex subunit 4, metazoa / HAUS complex subunit 5, metazoa / HAUS augmin-like complex subunit 1 ...HAUS augmin-like complex subunit 2, metazoa / HAUS augmin-like complex subunit 6 / HAUS augmin-like complex subunit 6, N-terminal / HAUS augmin-like complex subunit 2 / HAUS augmin-like complex subunit 7-like / HAUS augmin-like complex subunit 6 N-terminus / HAUS augmin-like complex subunit 2 / HAUS augmin-like complex subunit 4, metazoa / HAUS complex subunit 5, metazoa / HAUS augmin-like complex subunit 1 / HAUS augmin-like complex subunit 5 / HAUS augmin-like complex subunit 4 / HAUS augmin-like complex subunit 4 / HAUS augmin-like complex subunit 5 / HAUS augmin-like complex subunit 3 / HAUS augmin-like complex subunit 3, N-terminal / HAUS augmin-like complex subunit 3 / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein
Similarity search - Domain/homology
HAUS augmin-like complex subunit 5 / HAUS augmin-like complex subunit 1 / HAUS augmin like complex subunit 6 L homeolog / HAUS augmin like complex subunit 7 S homeolog / Green fluorescent protein / HAUS augmin-like complex subunit 8 / HAUS augmin like complex subunit 4 L homeolog / HAUS augmin-like complex subunit 3 / HAUS augmin like complex subunit 2 L homeolog
Similarity search - Component
Biological speciesXenopus laevis (African clawed frog)
Methodsingle particle reconstruction / cryo EM / Resolution: 6.88 Å
AuthorsTravis SM / Huang W / Zhang R / Petry S
Funding support United States, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)F32GM142149 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)1R01GM138854 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)1R01GM141100 United States
CitationJournal: Nat Commun / Year: 2023
Title: Integrated model of the vertebrate augmin complex.
Authors: Sophie M Travis / Brian P Mahon / Wei Huang / Meisheng Ma / Michael J Rale / Jodi Kraus / Derek J Taylor / Rui Zhang / Sabine Petry /
Abstract: Accurate segregation of chromosomes is required to maintain genome integrity during cell division. This feat is accomplished by the microtubule-based spindle. To build a spindle rapidly and with high ...Accurate segregation of chromosomes is required to maintain genome integrity during cell division. This feat is accomplished by the microtubule-based spindle. To build a spindle rapidly and with high fidelity, cells take advantage of branching microtubule nucleation, which rapidly amplifies microtubules during cell division. Branching microtubule nucleation relies on the hetero-octameric augmin complex, but lack of structure information about augmin has hindered understanding how it promotes branching. In this work, we combine cryo-electron microscopy, protein structural prediction, and visualization of fused bulky tags via negative stain electron microscopy to identify the location and orientation of each subunit within the augmin structure. Evolutionary analysis shows that augmin's structure is highly conserved across eukaryotes, and that augmin contains a previously unidentified microtubule binding site. Thus, our findings provide insight into the mechanism of branching microtubule nucleation.
History
DepositionDec 1, 2022-
Header (metadata) releaseApr 19, 2023-
Map releaseApr 19, 2023-
UpdateApr 26, 2023-
Current statusApr 26, 2023Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_28981.png

Downloads & links

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Map

FileDownload / File: emd_28981.map.gz / Format: CCP4 / Size: 536.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationSharpened map of X laevis augmin
Voxel sizeX=Y=Z: 1.37 Å
Density
Contour LevelBy AUTHOR: 0.161
Minimum - Maximum-0.7487022 - 1.2238625
Average (Standard dev.)-0.000103659186 (±0.01917534)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions520520520
Spacing520520520
CellA=B=C: 712.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Half-map A of X laevis augmin

Fileemd_28981_half_map_1.map
AnnotationHalf-map A of X laevis augmin
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half-map B of X laevis augmin

Fileemd_28981_half_map_2.map
AnnotationHalf-map B of X laevis augmin
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Augmin

EntireName: Augmin
Components
  • Complex: Augmin
    • Protein or peptide: HAUS augmin-like complex subunit 1
    • Protein or peptide: HAUS augmin-like complex subunit 3
    • Protein or peptide: HAUS augmin like complex subunit 4 L homeolog
    • Protein or peptide: HAUS augmin-like complex subunit 5
    • Protein or peptide: HAUS augmin like complex subunit 2 L homeolog, Green fluorescent protein chimera
    • Protein or peptide: HAUS augmin like complex subunit 6 L homeolog
    • Protein or peptide: HAUS augmin like complex subunit 7 S homeolog
    • Protein or peptide: HAUS augmin-like complex subunit 8

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Supramolecule #1: Augmin

SupramoleculeName: Augmin / type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: all / Details: Peak octameric fraction following gel filtration
Source (natural)Organism: Xenopus laevis (African clawed frog) / Organelle: mitotic spindle / Location in cell: cytoplasm
Molecular weightTheoretical: 403 KDa

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Macromolecule #1: HAUS augmin-like complex subunit 1

MacromoleculeName: HAUS augmin-like complex subunit 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 32.655621 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MDEKSTKIIM WLKKMFGDKP LPPYEVNTRT MEILYQLAEW NEARDKDLSL VTEDLKLKSA EVKAEAKYLQ DLLTEGLGPS YTNLSRMGN NYLNQIVDSC LALELKNSSL SSYIPAVNDL SSELVAIELN NQEMEAELTS LRKKLTEALV LEKSLEQDLK K AEEQCNFE ...String:
MDEKSTKIIM WLKKMFGDKP LPPYEVNTRT MEILYQLAEW NEARDKDLSL VTEDLKLKSA EVKAEAKYLQ DLLTEGLGPS YTNLSRMGN NYLNQIVDSC LALELKNSSL SSYIPAVNDL SSELVAIELN NQEMEAELTS LRKKLTEALV LEKSLEQDLK K AEEQCNFE KAKVEIRSQN MKKLKDKSEE YKYKIHAAKD QLSSAGMEEP LTHRSLVSLS ETLTELKAQS MAAKEKLNSY LD LAPNPSL VKVKIEEAKR ELKATEVELT TKVNMMEFVV PEPSKRRLK

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Macromolecule #2: HAUS augmin-like complex subunit 3

MacromoleculeName: HAUS augmin-like complex subunit 3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 68.11225 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MSGGDRFVQT LQKLNYPKGA QLDGEDFDWL FEAVDLKPFL DWFCSAASEQ NVVPDEKLQA FNTLKESGKP VLDEKALDEV LKTFSISKV PAIEEVAIEK LEEEVKALQK QKNLHIRRRN KLQMVESGNR QMCLKSKDKE EETGRAFQEV LHLLRVTNKK L NHELQSIV ...String:
MSGGDRFVQT LQKLNYPKGA QLDGEDFDWL FEAVDLKPFL DWFCSAASEQ NVVPDEKLQA FNTLKESGKP VLDEKALDEV LKTFSISKV PAIEEVAIEK LEEEVKALQK QKNLHIRRRN KLQMVESGNR QMCLKSKDKE EETGRAFQEV LHLLRVTNKK L NHELQSIV NGVQTLMSFF STPETACELS SQPIFLSQLL LDKYLSLEEQ STAALTSFTK EHFFEGMSKF VEGSDENFQL VQ LNVNSFG EDGTTEDKCK EMMRLQLAYI CAKHKLIQMK AKSASLKVGL QWAENNASVV QDKASQKEEN LKVRITSLKN ETL QIENHT NSISNEKLPG LVRDNAQLLN MPIVKGDYDL QMAHQTSCSS RQDLVCDHLM KQKASFELLQ LGYELELRKH RDVY RELGS IVQELKESGD KLEERLTMLS DVNLLSASKP RSNIDSKDLT SHRLYQLLDG DNTQKLFRTY DGLESVAQKL SQDIA SMRD QLEVSEQEHS LLLSKLDSHL KELRDFMYPE GNTLMLTTPE LSGEFHQLGS QLEKLNHITV EILGDLQLKR KMLESN KLQ QIEKQLYVYF FQNEEQLKSI VGKLEAQTGG GSSA

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Macromolecule #3: HAUS augmin like complex subunit 4 L homeolog

MacromoleculeName: HAUS augmin like complex subunit 4 L homeolog / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 41.256438 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MAQTLQYVSS RLSMLQIDEE DLERNAQFGK VLIELCPLLG PNGGSANLNR ELEETRRELL LQRKMWMRSE VIYQLVQEML LDLQVRKLE GSLTEEERKF QDGLQQCMLV SECSRLLTAD SVPPSDSTSI LGLDKQDLLD LLPPNMLVLW VRDRLQKQLE E ALKKKCFT ...String:
MAQTLQYVSS RLSMLQIDEE DLERNAQFGK VLIELCPLLG PNGGSANLNR ELEETRRELL LQRKMWMRSE VIYQLVQEML LDLQVRKLE GSLTEEERKF QDGLQQCMLV SECSRLLTAD SVPPSDSTSI LGLDKQDLLD LLPPNMLVLW VRDRLQKQLE E ALKKKCFT FLSFHQPETD EEGDVLRAAK VLRLASTLED EKRRLQNEQE KHQEMRALLE KQQEIYPHVL LRCLSLLRQA AS ELRLRAQ SDIDRINAEY LEAKSNALFL KLRMEELQVL TDCYTPEKVL VHRQIRDTLE AGVKKEKQEL STSRQILSSY EFL GPEFEG LVQEYTRLKD KIKDNRWMLQ ELSKSLP

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Macromolecule #4: HAUS augmin-like complex subunit 5

MacromoleculeName: HAUS augmin-like complex subunit 5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 77.357281 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MERRSLAQEL KKWAVEEMGL PAQKAPSEEM LQRLFIGQCG DIWKFIIRHI HSHRTVRKIE GNLLWYQQLQ HTEAQRTAEE EQQQRRKQL CKEILELRAE LHHLQEQIQT AEREIVGQDL NCERAQDLCR RSLLLRAFNK KREEECEALC QSNKKIQYRC E QLQEIRRA ...String:
MERRSLAQEL KKWAVEEMGL PAQKAPSEEM LQRLFIGQCG DIWKFIIRHI HSHRTVRKIE GNLLWYQQLQ HTEAQRTAEE EQQQRRKQL CKEILELRAE LHHLQEQIQT AEREIVGQDL NCERAQDLCR RSLLLRAFNK KREEECEALC QSNKKIQYRC E QLQEIRRA SQREVMFSAV DPDLSSSTFL EPEVLRDVRE VCKLRFKFLR SLHDDSISSS VHPGKEDLRS LSHQQWMSMA EK VWNTHTP NHILAALERL TLNSTQELKK LQFSQAADLS KGPSCQLKEF SEPITQSRSC NESTHLDPQE TLPSFHSLIQ EGW ANSVKV SSELRRVQSQ AQALSEHLAE RIQEIHKKLS DGSEVSVLTR AAFDAELRCV ILRGCRDALM QECRMLQEEA AGKK QEMKL LQQQQQNIQE ACLLLDKKQK HIQILIKGNS SSKSQIRRSS VEAQKYVQDK LLPWPQEIIQ ESQRLQDSIQ KEVKH FSAI CLPALLKVST DGFNLLPSRE LSINRMSNTH APYYGIFKGI YESVRLPLYK APESVLSHVA DMKKQLFFLR SQLSSR SEA ISKTQRALQK NTNPDTDALL KSLSDHYSLE LDEMVPKMQR LIQQCEKHQE YGKEVQATVM DWWEQPVQLC LPSEERG GL TLRQWRERWT VAVTALQRAT GSRS

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Macromolecule #5: HAUS augmin like complex subunit 2 L homeolog, Green fluorescent ...

MacromoleculeName: HAUS augmin like complex subunit 2 L homeolog, Green fluorescent protein chimera
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 53.505473 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MMAANPWSPV QPSAASLLLE KCLAAGVLSQ NALDQAQLEA PCFSHAEELQ RITEIKAEIN QKSLELELLR LEKETADITH PLYLGQKHQ ALQAMNSHLE AVLREKRSLR QRLAQPVCQE NLPIEASYHR FTAELLPLAV NFIEKLEIYI KTIQTIPKIP D CASNMDNA ...String:
MMAANPWSPV QPSAASLLLE KCLAAGVLSQ NALDQAQLEA PCFSHAEELQ RITEIKAEIN QKSLELELLR LEKETADITH PLYLGQKHQ ALQAMNSHLE AVLREKRSLR QRLAQPVCQE NLPIEASYHR FTAELLPLAV NFIEKLEIYI KTIQTIPKIP D CASNMDNA LMRMESLEAE MEEVTEQILT WREQQKTAFQ MNSDANSSCI TAQTSYLSIE NLHPVDMVSK GEELFTGVVP IL VELDGDV NGHKFSVSGE GEGDATYGKL TLKFICTTGK LPVPWPTLVT TLTYGVQCFS RYPDHMKQHD FFKSAMPEGY VQE RTIFFK DDGNYKTRAE VKFEGDTLVN RIELKGIDFK EDGNILGHKL EYNYNSHNVY IMADKQKNGI KVNFKIRHNI EDGS VQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL KEFVTAAGIT LGMDELYKVD HHHHHHH

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Macromolecule #6: HAUS augmin like complex subunit 6 L homeolog

MacromoleculeName: HAUS augmin like complex subunit 6 L homeolog / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 50.927965 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: GPASGSTRGA EFMQSGSRPH LAWQREHMWL ALQGLGFESG AEAANAGKTL VHVTFGVNMF DKPNKDAFYV VFHFLFGKLD NVRCKEVFR YCWPPLDKKR DAEFRKACCE WLKKISDEVG AGFPQVVASI FLSPGGPKFV HLLYHFARYV MLQHIKRDAD A GNVFISEA ...String:
GPASGSTRGA EFMQSGSRPH LAWQREHMWL ALQGLGFESG AEAANAGKTL VHVTFGVNMF DKPNKDAFYV VFHFLFGKLD NVRCKEVFR YCWPPLDKKR DAEFRKACCE WLKKISDEVG AGFPQVVASI FLSPGGPKFV HLLYHFARYV MLQHIKRDAD A GNVFISEA LQSKIQDPQK ALARNKLARQ KYLKVLQKEN LVIEEYQRKA QLLIKQIRDM RSEHVALQNQ QKLAEKVDRK IS DKDENIQ KTRCMWNTIM QMLKEMEKEV DVVDAVVRGN IDQYCLDGTN ATLNIPNLLI SRIESEMHRL QMDNVYEAGK VNL ITVVQL LNEALKLVSG ERSLYDCKGV RLDLQYLHGK AKFESEVLTR LRNMRHKIKR EDLVSIEKII ADREREWERK WEKI LGKCP FSLLKGLNPA LELNPPMAPF SFDPASEEVL KSSVFCH

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Macromolecule #7: HAUS augmin like complex subunit 7 S homeolog

MacromoleculeName: HAUS augmin like complex subunit 7 S homeolog / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 39.379605 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MTGGKELGAA VELYERLQML SCPCLEGVYL TDPQSIYELL CTPSSHRLDI LQWLCSRIYP PVQEQLSSLK ESQTDTKVKE IAKLCFDLM LCHFDDLDLI RGHASPFKQI SFIGQLLDVI QYPDTISSNV ILESLSHSTE KNVVTCIREN EELLKELFSS P HFQATLSP ...String:
MTGGKELGAA VELYERLQML SCPCLEGVYL TDPQSIYELL CTPSSHRLDI LQWLCSRIYP PVQEQLSSLK ESQTDTKVKE IAKLCFDLM LCHFDDLDLI RGHASPFKQI SFIGQLLDVI QYPDTISSNV ILESLSHSTE KNVVTCIREN EELLKELFSS P HFQATLSP ECNPWPADFK PLLNAEESLQ KRATQSSKGK DMSNSVEALL EISSSLKALK EECVDLCSSV TDGDKVIQSL RL ALTDFHQ LTIAFNQIYA NEFQEHCGHP APHMSPMGPF FQFVHQSLST CFKELESIAQ FTETSENIVD VVRERHQSKE KWA GSTIST LCEKMKELRQ SYEAFQQSSL QD

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Macromolecule #8: HAUS augmin-like complex subunit 8

MacromoleculeName: HAUS augmin-like complex subunit 8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Xenopus laevis (African clawed frog)
Molecular weightTheoretical: 41.144852 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MSEAGVAPIE DGSQNSSGGS SGDAALKKSK GGAKVVKSRY MQIGRSKVSK NSLANTTVCS GGKVPERGSG GTPTRRSLAP HKAKITAAV PLPALDGSIF TKEDLQSTLL DGHRIARPDL DLSVINDRTL QKITPRPVVT SEQKKPKRDT TPVNLVPEDM V EMIESQTL ...String:
MSEAGVAPIE DGSQNSSGGS SGDAALKKSK GGAKVVKSRY MQIGRSKVSK NSLANTTVCS GGKVPERGSG GTPTRRSLAP HKAKITAAV PLPALDGSIF TKEDLQSTLL DGHRIARPDL DLSVINDRTL QKITPRPVVT SEQKKPKRDT TPVNLVPEDM V EMIESQTL LLTYLTIKMQ KNLFRLEEKA ERNLLLVNDQ KDQLQETIHM MKRDLTLLQR EERLRDLIEK QDEVLTPVVT SK DPFKDNY TTFATALDST RHQLAIKNIH ITGNRHRYLE ELQKHLAITK SLLEEIMPSH ASENAESFDT IKDLENIVLK TDE ELARSF RQILDLSFKV NKEISLQSQK AVEETCESAL VRQWYFDGSL P

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.06 mg/mL
BufferpH: 7.7
Component:
ConcentrationName
10.0 mMHEPES
100.0 mMKCl
5.0 mMEGTA
2.0 mMMgCl2
1.0 mMDTT
0.05 % (v/v)NP-40
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - #0 - Film type ID: 1 / Support film - #0 - Material: CARBON / Support film - #0 - topology: HOLEY / Support film - #0 - Film thickness: 10.0 nm / Support film - #1 - Film type ID: 2 / Support film - #1 - Material: CARBON / Support film - #1 - topology: CONTINUOUS / Support film - #1 - Film thickness: 5.0 nm / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 8 sec.
VitrificationCryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 39.903 µm / Nominal defocus min: 10.105 µm / Nominal magnification: 64000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 2350 / Average electron dose: 66.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 2046060
Details: Template picks, trained on evenly-spaced templates from ab initio model
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. v3.3.2+220824)
Final 3D classificationNumber classes: 100 / Software - Name: cryoSPARC (ver. v3.3.2+220824)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. v3.3.2+220824)
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 6.88 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. v3.3.2+220824) / Number images used: 114000
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT / Target criteria: Cross-correlation coefficient
Output model

PDB-8fck:
Structure of the vertebrate augmin complex

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