EMDB-28634: Hypopseudouridylated yeast 80S bound with Taura syndrome virus (T...

Basic information

Entry

Database: EMDB / ID: EMD-28634

• Title: Hypopseudouridylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome entry site (IRES), eEF2, GDP, and sordarin, Structure II
• Map data: Hypopseudouridylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome entry site (IRES), eEF2, GDP, and sordarin, Structure II

Sample

• Complex: Hypopseudouridylated ribosome with TSV IRES, eEF2 and GDP
  • Protein or peptide: x 77 types
  • RNA: x 5 types
• Ligand: x 4 types

• Keywords: rRNA pseudouridylation / IRES initiation / confomation / eEF2 / RIBOSOME / Ribosome-RNA complex

Function / homology

Function:

Peptide chain elongation / Synthesis of diphthamide-EEF2 / positive regulation of translational elongation / ribosomal subunit / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / Protein methylation / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / nonfunctional rRNA decay / hexon binding / pre-mRNA 5'-splice site binding / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Ribosomal scanning and start codon recognition / response to cycloheximide / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA destabilization / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / negative regulation of mRNA splicing, via spliceosome / preribosome, large subunit precursor / regulation of amino acid metabolic process / L13a-mediated translational silencing of Ceruloplasmin expression / translational elongation / ribosomal large subunit export from nucleus / 90S preribosome / G-protein alpha-subunit binding / positive regulation of protein kinase activity / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of translational fidelity / protein-RNA complex assembly / translation elongation factor activity / ribosomal subunit export from nucleus / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / Neutrophil degranulation / cellular response to amino acid starvation / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / translational initiation / macroautophagy / protein kinase C binding / positive regulation of apoptotic signaling pathway / maintenance of translational fidelity / modification-dependent protein catabolic process / cytoplasmic stress granule / protein tag activity / rRNA processing / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosome biogenesis / viral capsid / ribosome binding / ribosomal small subunit assembly / protein-folding chaperone binding / small ribosomal subunit / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / ribosomal large subunit assembly / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / cytoplasmic translation / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / ribonucleoprotein complex / translation / negative regulation of gene expression / response to antibiotic / GTPase activity / mRNA binding / ubiquitin protein ligase binding / host cell nucleus / GTP binding / nucleolus / mitochondrion / RNA binding / zinc ion binding / nucleoplasm / identical protein binding / nucleus / metal ion binding / cytosol

Domain/homology:

60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / Pre-hexon-linking protein VIII / Adenovirus hexon associated protein, protein VIII / Elongation Factor G, domain II / Elongation Factor G, domain III / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / Elongation factor G C-terminus / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / 50S ribosomal protein L10, insertion domain superfamily / : / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / EF-G domain III/V-like / Tr-type G domain, conserved site / Translational (tr)-type guanine nucleotide-binding (G) domain signature. / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S21e signature. / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein L1, conserved site / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L1 signature. / Ribosomal protein L1 / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Small (40S) ribosomal subunit Asc1/RACK1 / S27a-like superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / 40S Ribosomal protein S10 / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L44e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein L10e / Ribosomal protein L13e / Ribosomal protein S27a / Ribosomal protein L13e / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein L19, eukaryotic / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S30 / : / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S7e signature. / : / Ribosomal protein L24e, conserved site / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L24e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / : / Ribosomal protein S19e / Ribosomal protein S3Ae, conserved site / Ribosomal protein S19e / Ribosomal protein L30e signature 1.

Component:

Rps5p / Small ribosomal subunit protein eS1 / RPS22A isoform 1 / RPS31 isoform 1 / RPL38 isoform 1 / RPL10 isoform 1 / RPS29A isoform 1 / RPS20 isoform 1 / RPS2 isoform 1 / 60S ribosomal protein L29 / RPL24A isoform 1 / 40S ribosomal protein S25 / 40S ribosomal protein S26 / RPL5 isoform 1 / RPL32 isoform 1 / Ribosomal protein / 40S ribosomal protein S3 / RPL4A isoform 1 / RPS15 isoform 1 / RPS28A isoform 1 / RPL11A isoform 1 / 40S ribosomal protein S12 / 60S ribosomal protein L20 / Small ribosomal subunit protein uS4A / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein eS17A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL22A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein eS21A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS30A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL2A / Small ribosomal subunit protein uS17A / Pre-hexon-linking protein VIII / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Small ribosomal subunit protein eS32A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL8A / Large ribosomal subunit protein uL13A / Small ribosomal subunit protein eS7A / Elongation factor 2 / Small ribosomal subunit protein uS2A / Small ribosomal subunit protein eS27A / Large ribosomal subunit protein eL14A / Small ribosomal subunit protein RACK1 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL34A / Large ribosomal subunit protein eL6A / Large ribosomal subunit protein eL21A / Small ribosomal subunit protein eS10A / Large ribosomal subunit protein eL13A

• Biological species: Saccharomyces cerevisiae (brewer's yeast) / Taura syndrome virus
• Method: single particle reconstruction / cryo EM / Resolution: 2.87 Å
• Authors: Zhao Y / Rai J / Li H

Funding support

United States, 1 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM124622	United States

• Citation: Journal: Sci Adv / Year: 2023; Title: Regulation of translation by ribosomal RNA pseudouridylation.; Authors: Yu Zhao / Jay Rai / Hong Li / ; Abstract: Pseudouridine is enriched in ribosomal, spliceosomal, transfer, and messenger RNA and thus integral to the central dogma. The chemical basis for how pseudouridine affects the molecular apparatus such as ribosome, however, remains elusive owing to the lack of structures without this natural modification. Here, we studied the translation of a hypopseudouridylated ribosome initiated by the internal ribosome entry site (IRES) elements. We analyzed eight cryo-electron microscopy structures of the ribosome bound with the Taura syndrome virus IRES in multiple functional states. We found widespread loss of pseudouridine-mediated interactions through water and long-range base pairings. In the presence of the translocase, eukaryotic elongation factor 2, and guanosine 5'-triphosphate hydrolysis, the hypopseudouridylated ribosome favors a rare unconducive conformation for decoding that is partially recouped in the ribosome population that remains modified at the P-site uridine. The structural principles learned establish the link between functional defects and modification loss and are likely applicable to other pseudouridine-associated processes.

History

Deposition	Oct 20, 2022	-
Header (metadata) release	Sep 6, 2023	-
Map release	Sep 6, 2023	-
Update	Nov 1, 2023	-
Current status	Nov 1, 2023	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_28634.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Hypopseudouridylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome entry site (IRES), eEF2, GDP, and sordarin, Structure II
• Voxel size: X=Y=Z: 1.06 Å

Density

Contour Level	By AUTHOR: 0.6
Minimum - Maximum	-1.7295684 - 4.409911
Average (Standard dev.)	0.05770027 (±0.23655964)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 423.99997 Å α=β=γ: 90.0 °

Supplemental data

Half map: Half Map 1

• File: emd_28634_half_map_1.map
• Annotation: Half Map 1

Half map: Half Map 2

• File: emd_28634_half_map_2.map
• Annotation: Half Map 2

Sample components

Entire : Hypopseudouridylated ribosome with TSV IRES, eEF2 and GDP

• Entire: Name: Hypopseudouridylated ribosome with TSV IRES, eEF2 and GDP

Components

• Complex: Hypopseudouridylated ribosome with TSV IRES, eEF2 and GDP
  • Protein or peptide: 40S ribosomal protein S0-A
  • Protein or peptide: RPS1A isoform 1
  • Protein or peptide: RPS2 isoform 1
  • Protein or peptide: 40S ribosomal protein S4-A
  • Protein or peptide: 40S ribosomal protein S6-A
  • Protein or peptide: 40S ribosomal protein S7-A
  • Protein or peptide: 40S ribosomal protein S8-A
  • Protein or peptide: 40S ribosomal protein S9-A
  • Protein or peptide: 40S ribosomal protein S11-A
  • Protein or peptide: 40S ribosomal protein S13
  • Protein or peptide: 40S ribosomal protein S14-A
  • Protein or peptide: 40S ribosomal protein S21-A
  • Protein or peptide: RPS22A isoform 1
  • Protein or peptide: 40S ribosomal protein S23-A
  • Protein or peptide: 40S ribosomal protein S24-A
  • Protein or peptide: RPS26B isoform 1
  • Protein or peptide: 40S ribosomal protein S27-A
  • Protein or peptide: 40S ribosomal protein S30-A
  • Protein or peptide: RPS3 isoform 1
  • Protein or peptide: Rps5p
  • Protein or peptide: 40S ribosomal protein S10-A
  • Protein or peptide: RPS15 isoform 1
  • Protein or peptide: 40S ribosomal protein S16-A
  • Protein or peptide: 40S ribosomal protein S17-A
  • Protein or peptide: 40S ribosomal protein S18-A
  • Protein or peptide: 40S ribosomal protein S19-A
  • Protein or peptide: RPS20 isoform 1
  • Protein or peptide: RPS25A isoform 1
  • Protein or peptide: RPS28A isoform 1
  • Protein or peptide: RPS29A isoform 1
  • Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
  • Protein or peptide: Ubiquitin-40S ribosomal protein S31
  • Protein or peptide: 40S ribosomal protein S12
  • RNA: 18S rRNA
  • Protein or peptide: 60S ribosomal protein L2-A
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: RPL4A isoform 1
  • RNA: 25S rRNA
  • RNA: 5s rRNA
  • RNA: 5.8 S rRNA
  • Protein or peptide: RPL5 isoform 1
  • Protein or peptide: 60S ribosomal protein L6-A
  • Protein or peptide: 60S ribosomal protein L7-A
  • Protein or peptide: 60S ribosomal protein L8-A
  • Protein or peptide: 60S ribosomal protein L9-A
  • Protein or peptide: RPL10 isoform 1
  • Protein or peptide: RPL11A isoform 1
  • Protein or peptide: 60S ribosomal protein L13-A
  • Protein or peptide: 60S ribosomal protein L14-A
  • Protein or peptide: 60S ribosomal protein L15-A
  • Protein or peptide: 60S ribosomal protein L16-A
  • Protein or peptide: 60S ribosomal protein L17-A
  • Protein or peptide: 60S ribosomal protein L18-A
  • Protein or peptide: 60S ribosomal protein L19-A
  • Protein or peptide: 60S ribosomal protein L20
  • Protein or peptide: 60S ribosomal protein L21-A
  • Protein or peptide: 60S ribosomal protein L22-A
  • Protein or peptide: 60S ribosomal protein L23-A
  • Protein or peptide: RPL24A isoform 1
  • Protein or peptide: 60S ribosomal protein L25
  • Protein or peptide: 60S ribosomal protein L26-A
  • Protein or peptide: 60S ribosomal protein L27-A
  • Protein or peptide: 60S ribosomal protein L28
  • Protein or peptide: RPL29 isoform 1
  • Protein or peptide: 60S ribosomal protein L30
  • Protein or peptide: 60S ribosomal protein L31-A
  • Protein or peptide: RPL32 isoform 1
  • Protein or peptide: 60S ribosomal protein L33-A
  • Protein or peptide: 60S ribosomal protein L34-A
  • Protein or peptide: 60S ribosomal protein L35-A
  • Protein or peptide: 60S ribosomal protein L36-A
  • Protein or peptide: 60S ribosomal protein L37-A
  • Protein or peptide: RPL38 isoform 1
  • Protein or peptide: 60S ribosomal protein L39
  • Protein or peptide: Ubiquitin-60S ribosomal protein L40
  • Protein or peptide: 60S ribosomal protein L41-A
  • Protein or peptide: 60S ribosomal protein L42-A
  • Protein or peptide: 60S ribosomal protein L43-A
  • Protein or peptide: RPL1A isoform 1
  • Protein or peptide: Elongation factor 2
  • Protein or peptide: 60S acidic ribosomal protein P0
  • RNA: TSV IRES
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE
• Ligand: (1S,3S,3aR,4S,4aR,7R,7aR,8aS)-8a-{[(6-deoxy-4-O-methyl-alpha-D-altropyranosyl)oxy]methyl}-4-formyl-7-methyl-3-(propan-2-yl)decahydro-1,4-methano-s-indacene-3a(1H)-carboxylate

Supramolecule #1: Hypopseudouridylated ribosome with TSV IRES, eEF2 and GDP

• Supramolecule: Name: Hypopseudouridylated ribosome with TSV IRES, eEF2 and GDP; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#82
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Macromolecule #1: 40S ribosomal protein S0-A

• Macromolecule: Name: 40S ribosomal protein S0-A / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 28.05133 KDa

Sequence

String:

MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV GKTWEKLVLA ARIIAAIPNP EDVVAISSRT FGQRAVLKF AAHTGATPIA GRFTPGSFTN YITRSFKEPR LVIVTDPRSD AQAIKEASYV NIPVIALTDL DSPSEFVDVA I PCNNRGKH SIGLIWYLLA REVLRLRGAL VDRTQPWSIM PDLYFYRDPE EVEQQVAEEA TTEEAGEEEA KEEVTEEQAE AT EWAEENA DNVEW

UniProtKB: Small ribosomal subunit protein uS2A

Macromolecule #2: RPS1A isoform 1

• Macromolecule: Name: RPS1A isoform 1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 28.798467 KDa

Sequence

String:

MAVGKNKRLS KGKKGQKKRV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKSASDA LKGRVVEVCL ADLQGSEDHS FRKIKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRHSYAQSS H IRAIRKVI SEILTKEVQG STLAQLTSKL IPEVINKEIE NATKDIFPLQ NIHVRKVKLL KQPKFDVGAL MALHGEGSGE EK GKKVTGF KDEVLETV

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #3: RPS2 isoform 1

• Macromolecule: Name: RPS2 isoform 1 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 27.490826 KDa

Sequence

String:

MSAPEAQQQK RGGFGGRNRG RPNRRGPRNT EEKGWVPVTK LGRLVKAGKI TTIEEIFLHS LPVKEFQIID TLLPGLQDEV MNIKPVQKQ TRAGQRTRFK AVVVVGDSNG HVGLGIKTAK EVAGAIRAGI IIAKLSVIPI RRGYWGTNLG QPHSLATKTT G KCGSVTVR LIPAPRGSGI VASPAVKKLL QLAGVEDVYT QSNGKTRTLE NTLKAAFVAI GNTYGFLTPN LWAEQPLPVS PL DIYSDEA SAQKKRF

UniProtKB: RPS2 isoform 1

Macromolecule #4: 40S ribosomal protein S4-A

• Macromolecule: Name: 40S ribosomal protein S4-A / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 29.46933 KDa

Sequence

String:

MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDV ITLDATNENF RLVYDVKGRF AVHRITDEEA SYKLGKVKKV QLGKKGVPYV VTHDGRTIRY PDPNIKVNDT V KIDLASGK ITDFIKFDAG KLVYVTGGRN LGRIGTIVHK ERHDGGFDLV HIKDSLDNTF VTRLNNVFVI GEQGKPYISL PK GKGIKLS IAEERDRRRA QQGL

UniProtKB: Small ribosomal subunit protein eS4A

Macromolecule #5: 40S ribosomal protein S6-A

• Macromolecule: Name: 40S ribosomal protein S6-A / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.054486 KDa

Sequence

String:

MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE RKAEKAEIRK RRASSLKA

UniProtKB: Small ribosomal subunit protein eS6A

Macromolecule #6: 40S ribosomal protein S7-A

• Macromolecule: Name: 40S ribosomal protein S7-A / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.658209 KDa

Sequence

String:

MSAPQAKILS QAPTELELQV AQAFVELENS SPELKAELRP LQFKSIREID VAGGKKALAI FVPVPSLAGF HKVQTKLTRE LEKKFQDRH VIFLAERRIL PKPSRTSRQV QKRPRSRTLT AVHDKILEDL VFPTEIVGKR VRYLVGGNKI QKVLLDSKDV Q QIDYKLES FQAVYNKLTG KQIVFEIPSE TH

UniProtKB: Small ribosomal subunit protein eS7A

Macromolecule #7: 40S ribosomal protein S8-A

• Macromolecule: Name: 40S ribosomal protein S8-A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 22.537803 KDa

Sequence

String:

MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWFEAHYGQ TLGKKKNVKE EETVAKSKNA ERKWAARAAS AKIESSVESQ F SAGRLYAC ISSRPGQSGR CDGYILEGEE LAFYLRRLTA KK

UniProtKB: Small ribosomal subunit protein eS8A

Macromolecule #8: 40S ribosomal protein S9-A

• Macromolecule: Name: 40S ribosomal protein S9-A / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.487893 KDa

Sequence

String:

MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKK EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRVGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI TQRHIAVGKQ IVNIPSFMVR LDSEKHIDFA P TSPFGGAR PGRVARRNAA RKAEASGEAA DEADEADEE

UniProtKB: Small ribosomal subunit protein uS4A

Macromolecule #9: 40S ribosomal protein S11-A

• Macromolecule: Name: 40S ribosomal protein S11-A / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 17.785934 KDa

Sequence

String:

MSTELTVQSE RAFQKQPHIF NNPKVKTSKR TKRWYKNAGL GFKTPKTAIE GSYIDKKCPF TGLVSIRGKI LTGTVVSTKM HRTIVIRRA YLHYIPKYNR YEKRHKNVPV HVSPAFRVQV GDIVTVGQCR PISKTVRFNV VKVSAAAGKA NKQFAKF

UniProtKB: Small ribosomal subunit protein uS17A

Macromolecule #10: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 17.059945 KDa

Sequence

String:

MGRMHSAGKG ISSSAIPYSR NAPAWFKLSS ESVIEQIVKY ARKGLTPSQI GVLLRDAHGV TQARVITGNK IMRILKSNGL APEIPEDLY YLIKKAVSVR KHLERNRKDK DAKFRLILIE SRIHRLARYY RTVAVLPPNW KYESATASAL VN

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #11: 40S ribosomal protein S14-A

• Macromolecule: Name: 40S ribosomal protein S14-A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.562655 KDa

Sequence

String:

MSNVVQARDN SQVFGVARIY ASFNDTFVHV TDLSGKETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH VKIRATGGT RTKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

UniProtKB: Small ribosomal subunit protein uS11A

Macromolecule #12: 40S ribosomal protein S21-A

• Macromolecule: Name: 40S ribosomal protein S21-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 9.758829 KDa

Sequence

String:

MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY VTYALSGYVR SRGESDDSLN RLAQNDGLLK NVWSYSR

UniProtKB: Small ribosomal subunit protein eS21A

Macromolecule #13: RPS22A isoform 1

• Macromolecule: Name: RPS22A isoform 1 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.650062 KDa

Sequence

String:

MTRSSVLADA LNAINNAEKT GKRQVLIRPS SKVIIKFLQV MQKHGYIGEF EYIDDHRSGK IVVQLNGRLN KCGVISPRFN VKIGDIEKW TANLLPARQF GYVILTTSAG IMDHEEARRK HVSGKILGFV Y

UniProtKB: RPS22A isoform 1

Macromolecule #14: 40S ribosomal protein S23-A

• Macromolecule: Name: 40S ribosomal protein S23-A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 16.073896 KDa

Sequence

String:

MGKGKPRGLN SARKLRVHRR NNRWAENNYK KRLLGTAFKS SPFGGSSHAK GIVLEKLGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VSLLALWKEK KEKPRS

UniProtKB: Small ribosomal subunit protein uS12A

Macromolecule #15: 40S ribosomal protein S24-A

• Macromolecule: Name: 40S ribosomal protein S24-A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.362848 KDa

Sequence

String:

MSDAVTIRTR KVISNPLLAR KQFVVDVLHP NRANVSKDEL REKLAEVYKA EKDAVSVFGF RTQFGGGKSV GFGLVYNSVA EAKKFEPTY RLVRYGLAEK VEKASRQQRK QKKNRDKKIF GTGKRLAKKV ARRNAD

UniProtKB: Small ribosomal subunit protein eS24A

Macromolecule #16: RPS26B isoform 1

• Macromolecule: Name: RPS26B isoform 1 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.480886 KDa

Sequence

String:

MPKKRASNGR NKKGRGHVKP VRCVNCSKSI PKDKAIKRMA IRNIVEAAAV RDLSEASVYP EYALPKTYNK LHYCVSCAIH ARIVRVRSR EDRKNRAPPQ RPRFNRDNKV SPAAAAKKAL

UniProtKB: 40S ribosomal protein S26

Macromolecule #17: 40S ribosomal protein S27-A

• Macromolecule: Name: 40S ribosomal protein S27-A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 8.893391 KDa

Sequence

String:

MVLVQDLLHP TAASEARKHK LKTLVQGPRS YFLDVKCPGC LNITTVFSHA QTAVTCESCS TILCTPTGGK AKLSEGTSFR RK

UniProtKB: Small ribosomal subunit protein eS27A

Macromolecule #18: 40S ribosomal protein S30-A

• Macromolecule: Name: 40S ribosomal protein S30-A / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 7.137541 KDa

Sequence

String:

MAKVHGSLAR AGKVKSQTPK VEKTEKPKKP KGRAYKRLLY TRRFVNVTLV NGKRRMNPGP SVQ

UniProtKB: Small ribosomal subunit protein eS30A

Macromolecule #19: RPS3 isoform 1

• Macromolecule: Name: RPS3 isoform 1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 26.542789 KDa

Sequence

String:

MVALISKKRK LVADGVFYAE LNEFFTRELA EEGYSGVEVR VTPTKTEVII RATRTQDVLG ENGRRINELT LLVQKRFKYA PGTIVLYAE RVQDRGLSAV AQAESMKFKL LNGLAIRRAA YGVVRYVMES GAKGCEVVVS GKLRAARAKA MKFADGFLIH S GQPVNDFI DTATRHVLMR QGVLGIKVKI MRDPAKSRTG PKALPDAVTI IEPKEEEPIL APSVKDYRPA EETEAQAEPV EA

UniProtKB: 40S ribosomal protein S3

Macromolecule #20: Rps5p

• Macromolecule: Name: Rps5p / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 25.0726 KDa

Sequence

String:

MSDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD YVQVRQPIFV AHTAGRYANK RFRKAQCPI IERLTNSLMM NGRNNGKKLK AVRIIKHTLD IINVLTDQNP IQVVVDAITN TGPREDTTRV GGGGAARRQA V DVSPLRRV NQAIALLTIG AREAAFRNIK TIAETLAEEL INAAKGSSTS YAIKKKDELE RVAKSNR

UniProtKB: Rps5p

Macromolecule #21: 40S ribosomal protein S10-A

• Macromolecule: Name: 40S ribosomal protein S10-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.757445 KDa

Sequence

String:

MLMPKEDRNK IHQYLFQEGV VVAKKDFNQA KHEEIDTKNL YVIKALQSLT SKGYVKTQFS WQYYYYTLTE EGVEYLREYL NLPEHIVPG TYIQERNPTQ RPQRRY

UniProtKB: Small ribosomal subunit protein eS10A

Macromolecule #22: RPS15 isoform 1

• Macromolecule: Name: RPS15 isoform 1 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 16.031907 KDa

Sequence

String:

MSQAVNAKKR VFKTHSYRGV DLEKLLEMST EDFVKLAPAR VRRRFARGMT SKPAGFMKKL RAAKLAAPEN EKPAPVRTHM RNMIIVPEM IGSVVGIYNG KAFNQVEIRP EMLGHYLGEF SITYTPVRHG RAGATTSRFI PLK

UniProtKB: RPS15 isoform 1

Macromolecule #23: 40S ribosomal protein S16-A

• Macromolecule: Name: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.87749 KDa

Sequence

String:

MSAVPSVQTF GKKKSATAVA HVKAGKGLIK VNGSPITLVE PEILRFKVYE PLLLVGLDKF SNIDIRVRVT GGGHVSQVYA IRQAIAKGL VAYHQKYVDE QSKNELKKAF TSYDRTLLIA DSRRPEPKKF GGKGARSRFQ KSYR

UniProtKB: Small ribosomal subunit protein uS9A

Macromolecule #24: 40S ribosomal protein S17-A

• Macromolecule: Name: 40S ribosomal protein S17-A / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.820413 KDa

Sequence

String:

MGRVRTKTVK RASKALIERY YPKLTLDFQT NKRLCDEIAT IQSKRLRNKI AGYTTHLMKR IQKGPVRGIS FKLQEEERER KDQYVPEVS ALDLSRSNGV LNVDNQTSDL VKSLGLKLPL SVINVSAQRD RRYRKRV

UniProtKB: Small ribosomal subunit protein eS17A

Macromolecule #25: 40S ribosomal protein S18-A

• Macromolecule: Name: 40S ribosomal protein S18-A / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 17.071641 KDa

Sequence

String:

MSLVVQEQGS FQHILRLLNT NVDGNIKIVY ALTTIKGVGR RYSNLVCKKA DVDLHKRAGE LTQEELERIV QIMQNPTHYK IPAWFLNRQ NDITDGKDYH TLANNVESKL RDDLERLKKI RAHRGIRHFW GLRVRGQHTK TTGRRRA

UniProtKB: Small ribosomal subunit protein uS13A

Macromolecule #26: 40S ribosomal protein S19-A

• Macromolecule: Name: 40S ribosomal protein S19-A / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.942125 KDa

Sequence

String:

MPGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA EGWFYKRAAS VARHIYMRKQ VGVGKLNKLY GGAKSRGVR PYKHIDASGS INRKVLQALE KIGIVEISPK GGRRISENGQ RDLDRIAAQT LEEDE

UniProtKB: Small ribosomal subunit protein eS19A

Macromolecule #27: RPS20 isoform 1

• Macromolecule: Name: RPS20 isoform 1 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.929044 KDa

Sequence

String:

MSDFQKEKVE EQEQQQQQII KIRITLTSTK VKQLENVSSN IVKNAEQHNL VKKGPVRLPT KVLKISTRKT PNGEGSKTWE TYEMRIHKR YIDLEAPVQI VKRITQITIE PGVDVEVVVA SN

UniProtKB: RPS20 isoform 1

Macromolecule #28: RPS25A isoform 1

• Macromolecule: Name: RPS25A isoform 1 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.067272 KDa

Sequence

String:

MPPKQQLSKA AKAAAALAGG KKSKKKWSKK SMKDRAQHAV ILDQEKYDRI LKEVPTYRYV SVSVLVDRLK IGGSLARIAL RHLEKEGII KPISKHSKQA IYTRATASE

UniProtKB: 40S ribosomal protein S25

Macromolecule #29: RPS28A isoform 1

• Macromolecule: Name: RPS28A isoform 1 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 7.605847 KDa

Sequence

String:

MDNKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTSRTI VRNVKGPVRE NDILVLMESE REARRLR

UniProtKB: RPS28A isoform 1

Macromolecule #30: RPS29A isoform 1

• Macromolecule: Name: RPS29A isoform 1 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.675723 KDa

Sequence

String:

MAHENVWFSH PRRYGKGSRQ CRVCSSHTGL IRKYGLNICR QCFREKANDI GFNKFR

UniProtKB: RPS29A isoform 1

Macromolecule #31: Guanine nucleotide-binding protein subunit beta-like protein

• Macromolecule: Name: Guanine nucleotide-binding protein subunit beta-like protein; type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 34.841219 KDa

Sequence

String:

MASNEVLVLR GTLEGHNGWV TSLATSAGQP NLLLSASRDK TLISWKLTGD DQKFGVPVRS FKGHSHIVQD CTLTADGAYA LSASWDKTL RLWDVATGET YQRFVGHKSD VMSVDIDKKA SMIISGSRDK TIKVWTIKGQ CLATLLGHND WVSQVRVVPN E KADDDSVT IISAGNDKMV KAWNLNQFQI EADFIGHNSN INTLTASPDG TLIASAGKDG EIMLWNLAAK KAMYTLSAQD EV FSLAFSP NRYWLAAATA TGIKVFSLDP QYLVDDLRPE FAGYSKAAEP HAVSLAWSAD GQTLFAGYTD NVIRVWQVMT AN

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #32: Ubiquitin-40S ribosomal protein S31

• Macromolecule: Name: Ubiquitin-40S ribosomal protein S31 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 17.254227 KDa

Sequence

String:

MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGGKKR KKKVYTTPK KIKHKHKKVK LAVLSYYKVD AEGKVTKLRR ECSNPTCGAG VFLANHKDRL YCGKCHSVYK VNA

UniProtKB: RPS31 isoform 1

Macromolecule #33: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.488631 KDa

Sequence

String:

MSDVEEVVEV QEETVVEQTA EVTIEDALKV VLRTALVHDG LARGLRESTK ALTRGEALLV VLVSSVTEAN IIKLVEGLAN DPENKVPLI KVADAKQLGE WAGLGKIDRE GNARKVVGAS VVVVKNWGAE TDELSMIMEH FSQQ

UniProtKB: 40S ribosomal protein S12

Macromolecule #35: 60S ribosomal protein L2-A

• Macromolecule: Name: 60S ribosomal protein L2-A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.463574 KDa

Sequence

String:

MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH DSGRGAPLAK VVFRDPYKYR LREEIFIANE GVHTGQFIY AGKKASLNVG NVLPLGSVPE GTIVSNVEEK PGDRGALARA SGNYVIIIGH NPDENKTRVR LPSGAKKVIS S DARGVIGV IAGGGRVDKP LLKAGRAFHK YRLKRNSWPK TRGVAMNPVD HPHGGGNHQH IGKASTISRG AVSGQKAGLI AA RRTGLLR GSQKTQD

UniProtKB: Large ribosomal subunit protein uL2A

Macromolecule #36: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 43.863812 KDa

Sequence

String:

MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL DRPGSKFHKR EVVEAVTVVD TPPVVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAQ DGAGIERELA RIKKYASVVR V LVHTQIRK TPLAQKKAHL AEIQLNGGSI SEKVDWAREH FEKTVAVDSV FEQNEMIDAI AVTKGHGFEG VTHRWGTKKL PR KT(HIC)RGLR KVACIGAWHP AHVMWSVARA GQRGYHSRTS INHKIYRVGK GDDEANGATS FDRTKKTITP MGGFVHYGE IKNDFIMVKG CIPGNRKRIV TLRKSLYTNT SRKALEEVSL KWIDTASKFG KGRFQTPAEK HAFMGTLKKD L

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #37: RPL4A isoform 1

• Macromolecule: Name: RPL4A isoform 1 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 39.159125 KDa

Sequence

String:

MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGA FGNMCRGGRM FAPTKTWRKW NVKVNHNEKR YATASAIAAT AVASLVLARG HRVEKIPEIP LVVSTDLESI Q KTKEAVAA LKAVGAHSDL LKVLKSKKLR AGKGKYRNRR WTQRRGPLVV YAEDNGIVKA LRNVPGVETA NVASLNLLQL AP GAHLGRF VIWTEAAFTK LDQVWGSETV ASSKVGYTLP SHIISTSDVT RIINSSEIQS AIRPAGQATQ KRTHVLKKNP LKN KQVLLR LNPYAKVFAA EKLGSKKAEK TGTKPAAVFT ETLKHD

UniProtKB: RPL4A isoform 1

Macromolecule #41: RPL5 isoform 1

• Macromolecule: Name: RPL5 isoform 1 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 33.764828 KDa

Sequence

String:

MAFQKDAKSS AYSSRFQTPF RRRREGKTDY YQRKRLVTQH KAKYNTPKYR LVVRFTNKDI ICQIISSTIT GDVVLAAAYS HELPRYGIT HGLTNWAAAY ATGLLIARRT LQKLGLDETY KGVEEVEGEY ELTEAVEDGP RPFKVFLDIG LQRTTTGARV F GALKGASD GGLYVPHSEN RFPGWDFETE EIDPELLRSY IFGGHVSQYM EELADDDEER FSELFKGYLA DDIDADSLED IY TSAHEAI RADPAFKPTE KKFTKEQYAA ESKKYRQTKL SKEERAARVA AKIAALAGQQ

UniProtKB: RPL5 isoform 1

Macromolecule #42: 60S ribosomal protein L6-A

• Macromolecule: Name: 60S ribosomal protein L6-A / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.000564 KDa

Sequence

String:

MSAQKAPKWY PSEDVAALKK TRKAARPQKL RASLVPGTVL ILLAGRFRGK RVVYLKHLED NTLLISGPFK VNGVPLRRVN ARYVIATST KVSVEGVNVE KFNVEYFAKE KLTKKEKKEA NLFPEQQNKE IKAERVEDQK VVDKALIAEI KKTPLLKQYL S ASFSLKNG DKPHMLKF

UniProtKB: Large ribosomal subunit protein eL6A

Macromolecule #43: 60S ribosomal protein L7-A

• Macromolecule: Name: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.686281 KDa

Sequence

String:

MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRI KGINKIPPKP RKVLQLLRLT RINSGTFVKV TKATLELLKL IEPYVAYGYP SYSTIRQLVY KRGFGKINKQ R VPLSDNAI IEANLGKYGI LSIDDLIHEI ITVGPHFKQA NNFLWPFKLS NPSGGWGVPR KFKHFIQGGS FGNREEFINK LV KSMN

UniProtKB: Large ribosomal subunit protein uL30A

Macromolecule #44: 60S ribosomal protein L8-A

• Macromolecule: Name: 60S ribosomal protein L8-A / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 28.17582 KDa

Sequence

String:

MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAE TFKLFNKYRP ETAAEKKERL TKEAAAVAEG KSKQDASPKP YAVKYGLNHV VALIENKKAK LVLIANDVDP I ELVVFLPA LCKKMGVPYA IVKGKARLGT LVNQKTSAVA ALTEVRAEDE AALAKLVSTI DANFADKYDE VKKHWGGGIL GN KAQAKMD KRAKNSDSA

UniProtKB: Large ribosomal subunit protein eL8A

Macromolecule #45: 60S ribosomal protein L9-A

• Macromolecule: Name: 60S ribosomal protein L9-A / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.605061 KDa

Sequence

String:

MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

UniProtKB: Large ribosomal subunit protein uL6A

Macromolecule #46: RPL10 isoform 1

• Macromolecule: Name: RPL10 isoform 1 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 25.410465 KDa

Sequence

String:

MARRPARCYR YQKNKPYPKS RYNRAVPDSK IRIYDLGKKK ATVDEFPLCV HLVSNELEQL SSEALEAARI CANKYMTTVS GRDAFHLRV RVHPFHVLRI NKMLSCAGAD RLQQGMRGAW GKPHGLAARV DIGQIIFSVR TKDSNKDVVV EGLRRARYKF P GQQKIILS KKWGFTNLDR PEYLKKREAG EVKDDGAFVK FLSKKGSLEN NIREFPEYFA AQA

UniProtKB: RPL10 isoform 1

Macromolecule #47: RPL11A isoform 1

• Macromolecule: Name: RPL11A isoform 1 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 19.755691 KDa

Sequence

String:

MSAKAQNPMR DLKIEKLVLN ISVGESGDRL TRASKVLEQL SGQTPVQSKA RYTVRTFGIR RNEKIAVHVT VRGPKAEEIL ERGLKVKEY QLRDRNFSAT GNFGFGIDEH IDLGIKYDPS IGIFGMDFYV VMNRPGARVT RRKRCKGTVG NSHKTTKEDT V SWFKQKYD ADVLDK

UniProtKB: RPL11A isoform 1

Macromolecule #48: 60S ribosomal protein L13-A

• Macromolecule: Name: 60S ribosomal protein L13-A / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.604164 KDa

Sequence

String:

MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRNARA TRAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEV KAAGLTAAY ARTIGIAVDH RRQNRNQEIF DANVQRLKEY QSKIIVFPRN GKAPEAEQVL SAAATFPIAQ PATDVEARAV Q DNGESAFR TLRLARSEKK FRGIREKRAR EKAEAEAEKK K

UniProtKB: Large ribosomal subunit protein eL13A

Macromolecule #49: 60S ribosomal protein L14-A

• Macromolecule: Name: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.195066 KDa

Sequence

String:

MSTDSIVKAS NWRLVEVGRV VLIKKGQSAG KLAAIVEIID QKKVLIDGPK AGVPRQAINL GQVVLTPLTF ALPRGARTAT VSKKWAAAA VCEKWAASSW AKKIAQRERR AALTDFERFQ VMVLRKQKRY TVKKALAKA

UniProtKB: Large ribosomal subunit protein eL14A

Macromolecule #50: 60S ribosomal protein L15-A

• Macromolecule: Name: 60S ribosomal protein L15-A / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 24.482357 KDa

Sequence

String:

MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAA RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVPKGAT YGKPTNQGV NELKYQRSLR ATAEERVGRR AANLRVLNSY WVNQDSTYKY FEVILVDPQH KAIRRDARYN WICDPVHKHR E ARGLTATG KKSRGINKGH KFNNTKAGRR KTWKRQNTLS LWRYRK

UniProtKB: Large ribosomal subunit protein eL15A

Macromolecule #51: 60S ribosomal protein L16-A

• Macromolecule: Name: 60S ribosomal protein L16-A / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.247227 KDa

Sequence

String:

MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVS HKTARGKAAL ERLKVFEGIP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEAK R KVSSAEYY AKKRAFTKKV ASANATAAES DVAKQLAALG Y

UniProtKB: Large ribosomal subunit protein uL13A

Macromolecule #52: 60S ribosomal protein L17-A

• Macromolecule: Name: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.589518 KDa

Sequence

String:

MARYGATSTN PAKSASARGS YLRVSFKNTR ETAQAINGWE LTKAQKYLEQ VLDHQRAIPF RRFNSSIGRT AQGKEFGVTK ARWPAKSVK FVQGLLQNAA ANAEAKGLDA TKLYVSHIQV NQAPKQRRRT YRAHGRINKY ESSPSHIELV VTEKEEAVAK A AEKKVVRL TSRQRGRIAA QKRIAA

UniProtKB: Large ribosomal subunit protein uL22A

Macromolecule #53: 60S ribosomal protein L18-A

• Macromolecule: Name: 60S ribosomal protein L18-A / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.609252 KDa

Sequence

String:

MGIDHTSKQH KRSGHRTAPK SDNVYLKLLV KLYTFLARRT DAPFNKVVLK ALFLSKINRP PVSVSRIARA LKQEGAANKT VVVVGTVTD DARIFEFPKT TVAALRFTAG ARAKIVKAGG ECITLDQLAV RAPKGQNTLI LRGPRNSREA VRHFGMGPHK G KAPRILST GRKFERARGR RRSKGFKV

UniProtKB: Pre-hexon-linking protein VIII

Macromolecule #54: 60S ribosomal protein L19-A

• Macromolecule: Name: 60S ribosomal protein L19-A / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.762316 KDa

Sequence

String:

MANLRTQKRL AASVVGVGKR KVWLDPNETS EIAQANSRNA IRKLVKNGTI VKKAVTVHSK SRTRAHAQSK REGRHSGYGK RKGTREARL PSQVVWIRRL RVLRRLLAKY RDAGKIDKHL YHVLYKESKG NAFKHKRALV EHIIQAKADA QREKALNEEA E ARRLKNRA ARDRRAQRVA EKRDALLKED A

UniProtKB: Large ribosomal subunit protein eL19A

Macromolecule #55: 60S ribosomal protein L20

• Macromolecule: Name: 60S ribosomal protein L20 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.279863 KDa

Sequence

String:

MPQKWKMAHF KEYQVIGRRL PTESVPEPKL FRMRIFASNE VIAKSRYWYF LQKLHKVKKA SGEIVSINQI NEAHPTKVKN FGVWVRYDS RSGTHNMYKE IRDVSRVAAV ETLYQDMAAR HRARFRSIHI LKVAEIEKTA DVKRQYVKQF LTKDLKFPLP H RVQKSTKT FSYKRPSTFY

UniProtKB: 60S ribosomal protein L20

Macromolecule #56: 60S ribosomal protein L21-A

• Macromolecule: Name: 60S ribosomal protein L21-A / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 18.279266 KDa

Sequence

String:

MGKSHGYRSR TRYMFQRDFR KHGAVHLSTY LKVYKVGDIV DIKANGSIQK GMPHKFYQGK TGVVYNVTKS SVGVIINKMV GNRYLEKRL NLRVEHIKHS KCRQEFLERV KANAAKRAEA KAQGVAVQLK RQPAQPRESR IVSTEGNVPQ TLAPVPYETF I

UniProtKB: Large ribosomal subunit protein eL21A

Macromolecule #57: 60S ribosomal protein L22-A

• Macromolecule: Name: 60S ribosomal protein L22-A / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.711359 KDa

Sequence

String:

MAPNTSRKQK IAKTFTVDVS SPTENGVFDP ASYAKYLIDH IKVEGAVGNL GNAVTVTEDG TVVTVVSTAK FSGKYLKYLT KKYLKKNQL RDWIRFVSTK TNEYRLAFYQ VTPEEDEEED EE

UniProtKB: Large ribosomal subunit protein eL22A

Macromolecule #58: 60S ribosomal protein L23-A

• Macromolecule: Name: 60S ribosomal protein L23-A / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.49395 KDa

Sequence

String:

MSGNGAQGTK FRISLGLPVG AIMNCADNSG ARNLYIIAVK GSGSRLNRLP AASLGDMVMA TVKKGKPELR KKVMPAIVVR QAKSWRRRD GVFLYFEDNA GVIANPKGEM KGSAITGPVG KECADLWPRV ASNSGVVV

UniProtKB: Large ribosomal subunit protein uL14A

Macromolecule #59: RPL24A isoform 1

• Macromolecule: Name: RPL24A isoform 1 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 17.661717 KDa

Sequence

String:

MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK KGITEEVAKK RSRKTVKAQR PITGASLDL IKERRSLKPE VRKANREEKL KANKEKKKAE KAARKAEKAK SAGTQSSKFS KQQAKGAFQK VAATSR

UniProtKB: RPL24A isoform 1

Macromolecule #60: 60S ribosomal protein L25

• Macromolecule: Name: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.787612 KDa

Sequence

String:

MAPSAKATAA KKAVVKGTNG KKALKVRTSA TFRLPKTLKL ARAPKYASKA VPHYNRLDSY KVIEQPITSE TAMKKVEDGN ILVFQVSMK ANKYQIKKAV KELYEVDVLK VNTLVRPNGT KKAYVRLTAD YDALDIANRI GYI

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #61: 60S ribosomal protein L26-A

• Macromolecule: Name: 60S ribosomal protein L26-A / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.265784 KDa

Sequence

String:

MAKQSLDVSS DRRKARKAYF TAPSSQRRVL LSAPLSKELR AQYGIKALPI RRDDEVLVVR GSKKGQEGKI SSVYRLKFAV QVDKVTKEK VNGASVPINL HPSKLVITKL HLDKDRKALI QRKGGKLE

UniProtKB: Large ribosomal subunit protein uL24A

Macromolecule #62: 60S ribosomal protein L27-A

• Macromolecule: Name: 60S ribosomal protein L27-A / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.56836 KDa

Sequence

String:

MAKFLKAGKV AVVVRGRYAG KKVVIVKPHD EGSKSHPFGH ALVAGIERYP LKVTKKHGAK KVAKRTKIKP FIKVVNYNHL LPTRYTLDV EAFKSVVSTE TFEQPSQREE AKKVVKKAFE ERHQAGKNQW FFSKLRF

UniProtKB: Large ribosomal subunit protein eL27A

Macromolecule #63: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 16.761666 KDa

Sequence

String:

MPSRFTKTRK HRGHVSAGKG RIGKHRKHPG GRGMAGGQHH HRINMDKYHP GYFGKVGMRY FHKQQAHFWK PVLNLDKLWT LIPEDKRDQ YLKSASKETA PVIDTLAAGY GKILGKGRIP NVPVIVKARF VSKLAEEKIR AAGGVVELIA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #64: RPL29 isoform 1

• Macromolecule: Name: RPL29 isoform 1 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 6.691884 KDa

Sequence

String:

MAKSKNHTAH NQTRKAHRNG IKKPKTYKYP SLKGVDPKFR RNHKHALHGT AKALAAAKK

UniProtKB: 60S ribosomal protein L29

Macromolecule #65: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 11.430364 KDa

Sequence

String:

MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV LRKSELEYYA MLSKTKVYYF QGGNNELGTA VGKLFRVGV VSILEAGDSD ILTTLA

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #66: 60S ribosomal protein L31-A

• Macromolecule: Name: 60S ribosomal protein L31-A / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.980158 KDa

Sequence

String:

MAGLKDVVTR EYTINLHKRL HGVSFKKRAP RAVKEIKKFA KLHMGTDDVR LAPELNQAIW KRGVKGVEYR LRLRISRKRN EEEDAKNPL FSYVEPVLVA SAKGLQTVVV EEDA

UniProtKB: Large ribosomal subunit protein eL31A

Macromolecule #67: RPL32 isoform 1

• Macromolecule: Name: RPL32 isoform 1 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.809441 KDa

Sequence

String:

MASLPHPKIV KKHTKKFKRH HSDRYHRVAE NWRKQKGIDS VVRRRFRGNI SQPKIGYGSN KKTKFLSPSG HKTFLVANVK DLETLTMHT KTYAAEIAHN ISAKNRVVIL ARAKALGIKV TNPKGRLALE A

UniProtKB: RPL32 isoform 1

Macromolecule #68: 60S ribosomal protein L33-A

• Macromolecule: Name: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.17713 KDa

Sequence

String:

MAESHRLYVK GKHLSYQRSK RVNNPNVSLI KIEGVATPQD AQFYLGKRIA YVYRASKEVR GSKIRVMWGK VTRTHGNSGV VRATFRNNL PAKTFGASVR IFLYPSNI

UniProtKB: Large ribosomal subunit protein eL33A

Macromolecule #69: 60S ribosomal protein L34-A

• Macromolecule: Name: 60S ribosomal protein L34-A / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.673196 KDa

Sequence

String:

MAQRVTFRRR NPYNTRSNKI KVVKTPGGIL RAQHVKKLAT RPKCGDCGSA LQGISTLRPR QYATVSKTHK TVSRAYGGSR CANCVKERI IRAFLIEEQK IVKKVVKEQT EAAKKSEKKA KK

UniProtKB: Large ribosomal subunit protein eL34A

Macromolecule #70: 60S ribosomal protein L35-A

• Macromolecule: Name: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.94264 KDa

Sequence

String:

MAGVKAYELR TKSKEQLASQ LVDLKKELAE LKVQKLSRPS LPKIKTVRKS IACVLTVINE QQREAVRQLY KGKKYQPKDL RAKKTRALR RALTKFEASQ VTEKQRKKQI AFPQRKYAIK A

UniProtKB: Large ribosomal subunit protein uL29A

Macromolecule #71: 60S ribosomal protein L36-A

• Macromolecule: Name: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 11.151259 KDa

Sequence

String:

MTVKTGIAIG LNKGKKVTSM TPAPKISYKK GAASNRTKFV RSLVREIAGL SPYERRLIDL IRNSGEKRAR KVAKKRLGSF TRAKAKVEE MNNIIAASRR H

UniProtKB: Large ribosomal subunit protein eL36A

Macromolecule #72: 60S ribosomal protein L37-A

• Macromolecule: Name: 60S ribosomal protein L37-A / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 9.877395 KDa

Sequence

String:

MGKGTPSFGK RHNKSHTLCN RCGRRSFHVQ KKTCSSCGYP AAKTRSYNWG AKAKRRHTTG TGRMRYLKHV SRRFKNGFQT GSASKASA

UniProtKB: Large ribosomal subunit protein eL37A

Macromolecule #73: RPL38 isoform 1

• Macromolecule: Name: RPL38 isoform 1 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.845561 KDa

Sequence

String:

MAREITDIKQ FLELTRRADV KTATVKINKK LNKAGKPFRQ TKFKVRGSSS LYTLVINDAG KAKKLIQSLP PTLKVNRL

UniProtKB: RPL38 isoform 1

Macromolecule #74: 60S ribosomal protein L39

• Macromolecule: Name: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 6.35864 KDa

Sequence

String:

MAAQKSFRIK QKMAKAKKQN RPLPQWIRLR TNNTIRYNAK RRNWRRTKMN I

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #75: Ubiquitin-60S ribosomal protein L40

• Macromolecule: Name: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.583077 KDa

Sequence

String:

MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLKALASKY NCDKSVCRKC YARLPPRATN CRKRKCGHTN QLRPKKKLK

UniProtKB: Ubiquitin-ribosomal protein eL40A fusion protein

Macromolecule #76: 60S ribosomal protein L41-A

• Macromolecule: Name: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 3.354243 KDa

Sequence

String:

MRAKWRKKRT RRLKRKRRKV RARSK

UniProtKB: Small ribosomal subunit protein eS32A

Macromolecule #77: 60S ribosomal protein L42-A

• Macromolecule: Name: 60S ribosomal protein L42-A / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.246658 KDa

Sequence

String:

MVNVPKTRKT YCKGKTCRKH TQHKVTQYKA GKASLFAQGK RRYDRKQSGF GGQTKPVFHK KAKTTKKVVL RLECVKCKTR AQLTLKRCK HFELGGEKKQ KGQALQF

UniProtKB: Large ribosomal subunit protein eL42A

Macromolecule #78: 60S ribosomal protein L43-A

• Macromolecule: Name: 60S ribosomal protein L43-A / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 10.112952 KDa

Sequence

String:

MAKRTKKVGI TGKYGVRYGS SLRRQVKKLE IQQHARYDCS FCGKKTVKRG AAGIWTCSCC KKTVAGGAYT VSTAAAATVR STIRRLREM VEA

UniProtKB: Large ribosomal subunit protein eL43A

Macromolecule #79: RPL1A isoform 1

• Macromolecule: Name: RPL1A isoform 1 / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.524799 KDa

Sequence

String:

MSKITSSQVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS GSLKLPNCPR PNMSICIFGD AFDVDRAKSC GVDAMSVDD LKKLNKNKKL IKKLSKKYNA FIASEVLIKQ VPRLLGPQLS KAGKFPTPVS HNDDLYGKVT DVRSTIKFQL K KVLCLAVA VGNVEMEEDV LVNQILMSVN FFVSLLKKNW QNVGSLVVKS SMGPAFRLY

UniProtKB: Ribosomal protein

Macromolecule #80: Elongation factor 2

• Macromolecule: Name: Elongation factor 2 / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 93.54932 KDa

Sequence

String:

MVAFTVDQMR SLMDKVTNVR NMSVIAHVDH GKSTLTDSLV QRAGIISAAK AGEARFTDTR KDEQERGITI KSTAISLYSE MSDEDVKEI KQKTDGNSFL INLIDSPGHV DFSSEVTAAL RVTDGALVVV DTIEGVCVQT ETVLRQALGE RIKPVVVINK V DRALLELQ VSKEDLYQTF ARTVESVNVI VSTYADEVLG DVQVYPARGT VAFGSGLHGW AFTIRQFATR YAKKFGVDKA KM MDRLWGD SFFNPKTKKW TNKDTDAEGK PLERAFNMFI LDPIFRLFTA IMNFKKDEIP VLLEKLEIVL KGDEKDLEGK ALL KVVMRK FLPAADALLE MIVLHLPSPV TAQAYRAEQL YEGPADDANC IAIKNCDPKA DLMLYVSKMV PTSDKGRFYA FGRV FAGTV KSGQKVRIQG PNYVPGKKDD LFIKAIQRVV LMMGRFVEPI DDCPAGNIIG LVGIDQFLLK TGTLTTSETA HNMKV MKFS VSPVVQVAVE VKNANDLPKL VEGLKRLSKS DPCVLTYMSE SGEHIVAGTG ELHLEICLQD LEHDHAGVPL KISPPV VAY RETVESESSQ TALSKSPNKH NRIYLKAEPI DEEVSLAIEN GIINPRDDFK ARARIMADDY GWDVTDARKI WCFGPDG NG PNLVIDQTKA VQYLHEIKDS VVAAFQWATK EGPIFGEEMR SVRVNILDVT LHADAI(DDE)RGG GQIIPTMRRA TYAG FLLAD PKIQEPVFLV EIQCPEQAVG GIYSVLNKKR GQVVSEEQRP GTPLFTVKAY LPVNESFGFT GELRQATGGQ AFPQM VFDH WSTLGSDPLD PTSKAGEIVL AARKRHGMKE EVPGWQEYYD KL

UniProtKB: Elongation factor 2

Macromolecule #81: 60S acidic ribosomal protein P0

• Macromolecule: Name: 60S acidic ribosomal protein P0 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 33.749121 KDa

Sequence

String:

MGGIREKKAE YFAKLREYLE EYKSLFVVGV DNVSSQQMHE VRKELRGRAV VLMGKNTMVR RAIRGFLSDL PDFEKLLPFV KGNVGFVFT NEPLTEIKNV IVSNRVAAPA RAGAVAPEDI WVRAVNTGME PGKTSFFQAL GVPTKIARGT IEIVSDVKVV D AGNKVGQS EASLLNLLNI SPFTFGLTVV QVYDNGQVFP SSILDITDEE LVSHFVSAVS TIASISLAIG YPTLPSVGHT LI NNYKDLL AVAIAASYHY PEIEDLVDRI ENPEKYAAAA PAATSAASGD AAPAEEAAAE EEEESDDDMG FGLFD

UniProtKB: Large ribosomal subunit protein uL10

Macromolecule #34: 18S rRNA

• Macromolecule: Name: 18S rRNA / type: rna / ID: 34 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 579.63525 KDa

Sequence

String:

UAUCUGGUUG AUCCUGCCAG UAGUCAU(A2M)UG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUA UAC AGUGAAACUG CGAAUGGCUC (A2M)UUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGU GGUAAU UCUAGAGCUA AUACAUGCUU AAAAUCUCGA CCCUUUGGAA GAGAUGUAUU UAUUAGAUAA AAAAUCAAUG UCUU CGGAC UCUUUGAUGA UUCAUAAUAA CUUUUCGAAU CGCAUGGCCU UGUGCUGGCG AUGGUUCAUU CAAAUUUCUG CCCUA UCAA CUUUCGAUGG UAGGAUAGUG GCCUACCAUG GUUUCAACGG GUAACGGGGA AUAAGGGUUC GAUUCCGGAG AGGGAG CCU GAGAAACGGC UACCACAU(OMC)C AAGG(A2M)AGGCA GCAGGCGCGC (A2M)AAUUACCCA AUCCUAAUUC AGGGA GGUA GUGACAAUAA AUAACGAUAC AGGGCCCAUU CGGGUCUUGU AAUUGGAAUG AGUACAAUGU AAAUACCUUA ACGAGG (A2M)AC AAUUGGAGGG CAAGUCUG(OMG)U GCCAGCAGCC GCGG(OMU)AAUUC CAGCUCCAAU AGCGUAUAUU AAAGU UGUU GCAGUU(A2M)AAA AGCUCGUAGU UGAACUUUGG GCCCGGUUGG CCGGUCCGAU UUUUCGUGUA CUGGAUUUCC AA CGGGGCC UUUCCUUCUG GCUAACCUUG AGUCCUUGUG GCUCUUGGCG AACCAGGACU UUUACUUUGA AAAAAUUAGA GUG UUCAAA GCAGGCGUAU UGCUCGAAUA UAUU(A2M)GCAUG GAAUAAUAGA AUAGGACGUU UGGUUCUAUU UUGUUGGUUU CUAGGACCA UCGUAAUGAU UAAUAGGGAC GGUCGGGGGC AUCAGUAUUC AAUUGUCAGA GGUGAAAUUC UUGGAUUUAU U GAAGACUA ACUACUGCGA AAGCAUUUGC CAAGGACGUU UUCAUUAAUC AAGA(A2M)CGAAA GUUAGGGGAU CGAAGAUG A UCAGAUAC(OMC)G UCGUAGUCUU AACCAUAAAC UAUGCCGACU AGGGAUCGGG UGGUGUUUUU UUAAUGACCC ACUCG GCAC CUUACGAGAA AUCAAAGUCU UUGGGUUCUG GGGGGAGUAU GGUCGCAA(OMG)G CUGAAACUUA AAGGAAUUGA CG GAAGGGC ACCACCAGGA GUGGAGCCUG CGGCUUAAUU UGAC(XSX)CAACA CGGGGAAACU CACCAGGUCC AGACACAAU AAGGAUUGAC AGAUUGAGAG CUCUUUCUUG AUUUUGUGGG UGG(OMU)G(OMG)UGCA UGGC(4AC)GUUCU UAGUUGGU G GAGUGAUUUG UCUGCUUAAU UGCGAUAACG AACGAGACCU UAACCUACUA AAUAGUGGUG CUAGCAUUUG CUGGUUAUC CACUUCUUAG AGGGACUAUC GGUUUCAAGC CGAUGGAAGU UUGAGGCAAU AACA(OMG)GUCUG UGAUGCCCUU AGACGU UCU GGGCCGCACG CGCGCUACAC UGACGGAGCC AGCGAGUCUA ACCUUGGCCG AGAGGUCUUG GUAAUCUUGU GAAACUC CG UCGUGCUGGG GAUAGAGCAU UGUAAUUAUU GCUCUUCAAC (OMG)AG(G7M)AAUUCC UAGUAAGCGC AAGUCAUCAG CUUGCGUUG AUUACGUCCC UGCCCUUUGU ACACACCG(OMC)C CGUCGCUAGU ACCGAUUGAA UGGCUUAGUG AGGCCUC AG GAUCUGCUUA GAGAAGGGGG CAACUCCAUC UCAGAGCGGA GAAUUUGGAC AAACUUGGUC AUUUAGAGGA ACUAAAAG U CGUAACAAGG UUUC(4AC)GUAGG UG(MA6)(MA6)CCUGCG GAAGGAUCAU U

GENBANK: GENBANK: CP022977.1

Macromolecule #38: 25S rRNA

• Macromolecule: Name: 25S rRNA / type: rna / ID: 38 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 1.0864791249999999 MDa

Sequence

String:

UUGACCUCAA AUCAGGUAGG AGUACCCGCU GAACUUAAGC AUAUCAAUAA GCGGAGGAAA AGAAACCAAC CGGGAUUGCC UUAGUAACG GCGAGUGAAG CGGCAAAAGC UCAAAUUUGA AAUCUGGUAC CUUCGGUGCC CGAGUUGUAA UUUGGAGAGG G CAACUUUG GGGCCGUUCC UUGUCUAUGU UCCUUGGAAC AGGACGUCAU AGAGGGUGAG AAUCCCGUGU GGCGAGGAGU GC GGUUCUU UGUAAAGUGC CUUCGAAGAG UCGAGUUGUU UGGGAAUGCA GCUCUAAGUG GGUGGUAAAU UCCAUCUAAA GCU AAAUAU UGGCGAGAGA CCGAUAGCGA ACAAGUACAG UGAUGGAAAG AUGAAAAGAA CUUUGAAAAG AGAGUGAAAA AGUA CGUGA AAUUGUUGAA AGGGAAGGGC AUUUGAUCAG ACAUGGUGUU UUGUAUUUCA CUGGCCAGCA UCAGUUUUGG UGGCA GGAU AAAUCCAUAG GAAUGUAGCU UGCCUCGGUA AGUAUUAUAG CCUGUGGGAA UACUGCCAGC UGGGACUGAG GACUGC GAC GUAAGUCAAG GAUGCUGGCA UAAUGGUUAU AUGCCGCCCG UCUUG(1MA)AAC(A2M) (OMC)GGACCAAGG AGU (OMC)UAACG UCUAUGCGAG UGUUUGGGUG UAAAACCCAU ACGCGUAAUG AAAGUGAACG UAGGUUGGGG CCUCGCAAGA GGUGCACAA UCGACCGAUC CUGAUGUCUU CGGAUGGAUU UGAGUAAGAG CAUAGCUGUU GGGACCC(OMG)A(A2M) AGA UGGUGA (A2M)CUAUGCCUG AAUAGGGUGA AGCCAGAGGA AACUCUGGUG GAGGCUCGUA (OMG)CGGUUCUG(A2M) CG UGCAAAU CGAUCGUCGA AU(OMU)UGGGUAU AG(OMG)GGCGAAA GACUAAUCGA ACCAUCUAGU AGCUGGUUCC UGCCG AAGU UUCCCUCAGG AUAGCAGAAG CUCGUAUCAG UUUUAUGAGG UAAAGCGAAU GAUUAGAGGU UCCGGGGUCG AAAUGA CCU UGACCUAUUC UCAAACUUUA AAUAUGUAAG AAGUCCUUGU UACUUAAUUG AACGUGGACA UUUGAAUGAA GAGCUUU UA GUGGGCCAUU UUUGGUAAGC (A2M)GAACUGGCG AUGCGGGAUG AACCGAACGU AGAGUUAAGG UGCCGGAAUA CACG CUCAU CAGACACCAC AAAAGGUGUU AGUUCAUCUA GACAGCCGGA CGGUGGCCAU GGAAGUCGGA AUCCGCUAAG GAGUG UGUA ACAACUCACC GGCCGAAUGA ACUAGCCCUG AAAAUGGAUG GCGCUCAAGC GUGUUACCUA UACUCUACCG UCAGGG UUG AUAUGAUGCC CUGACGAGUA GGCAGGCGUG GAGGUCAGUG ACGAAGCCUA GACCGUAAGG UCGGGUCGAA CGGCCUC UA GUGCAGAU(OMC)U UGGUGGUAGU (A2M)(OMG)CAAAUAUU CAAAUGAGAA CUUUGAAGAC UGAAGUGGGG AAAGGU UCC ACGUCAACAG CAGUUGGACG UGGGUUAGUC GAUCCUAAGA GAUGGGGAAG CUCCGUUUCA AAGGCCUGAU UUUAUGC AG GCCACCAUCG AAAGGGAAUC CGGUUAAGAU UCCGGAACCU GGAUAUGGAU UCUUCACGGU AACGUAACUG AAUGUGGA G ACGUCGGCGC GAGCCCUGGG AGGAGUUAUC UUUUCUUCUU AACAGCUUAU CACCCCGGAA UUGGUUUAUC CGGAGAUGG GGUCUUAUGG CUGGAAGAGG CCAGCACCUU UGCUGGCUCC GGUGCGCUUG UGACGGCCCG UGAAAAUCCA CAGGAAGGAA UAGUUUUCA UGCCAGGUCG UACUGAUAAC CGCAGCAGGU CUCCAAGGUG AACAGCCUCU AGUUGAUAGA AUAA(OMU)GU AG AUAAGGGAAG UCGGCAAAAU AGAUCCGUAA CUUCGGGAUA AGGAUUGGCU CUAAGGGUCG GGUAGUGAGG GCCUUGGU C AGACGCAGCG GGCGUGCUUG UGGACUGCUU GGUGGGGCUU GCUCUGCUAG GCGGACUACU UGCGUGCCUU GUUGUAGAC GGCCUUGGUA GGUCUCUUGU AGACCGUCGC UUGCUACAAU UAACGAUCAA CUUAGAACUG GUACGGACAA GGGGAAUCUG ACUGUCUAA UU(1MA)AAACAUA GCAUUGCGAU GGUCAGAAAG UGAUGUUGAC GCAAUGUGAU UUCUGCC(OMC)AG UGC UCUGAA UGUCAAAGUG A(A2M)GAAAUUCA ACCAAGCGCG GGUAAACGGC GGGAGUAACU AUGACUCUCU UAAGGUAGC (5MC)A(A2M)(A2M)UGCCUC (OMG)UCAUCUAAU UAGUGACGCG CAUGAAUGGA UUAACGAGAU UCCCACUGU(OMC) CCUAUCUAC (OMU)AUCUAGCGA AACCACAGCC AAGGGAACGG GCUUGGCAGA AUCAGCGGGG AAAGAAGACC CUGUUGA GC U(OMU)GAC(OMU)CUAG UUUGACAUUG UGAAGAGACA UAGAGGGUGU AGAAUAAGUG GGAGCUUCGG CGCCAGUGAA AUACCACUA CCUUUAUAGU UUCUUUACUU AUUCAAUGAA GCGGAGCUGG AAUUCAUUUU CCACGUUCUA GCAUUCAAGG U CCCAUUCG GGGCUGAUCC GGGUUGAAGA CAUUGUCAGG UGGGGAGUUU GGCUG(OMG)GGCG GCACAUCUGU (UR3)AAA CG(A2M)UA ACGCAGAUGU CCUAAGGGGG GCUCAUGGAG AACAGAAAUC UCCAGUAGAA CAAAAGGGUA AAAGCCCCCU U GAUUUUGA UU(OMU)UCAG(OMU)GU GAAUACAAAC CAUGAAAGUG UGGCCUAUCG AUCCUUUAGU CCCUCGGAAU UUGA GGCUA (OMG)A(OMG)GUGCCAG AAAAGUUACC ACAG(OMG)GAUAA CUGGCUUGUG GCAGUCAAGC GUUCAUAGCG ACA UUGCUU UUUGAUUCUU (5MC)GAUGUCGGC UCUUCCUAUC AUACCGAAGC AGAAUUCGGU AAGCGUUGGA U(OMU) (OMG)UUCACC CACUAAUAGG GAACGUG(A2M)G(OMC) UGGGUUUAGA (OMC)CGUCGUGAG ACAGGUUAGU UUUACCC UA CUGAUGAAUG UUACCGCAAU AGUAAUUGAA CUUAGUACGA GAGGAACAGU UCAUUCGGAU AAUUGGUUUU UGCGGCUG U CUGAUCAGGC AUUGCCGCGA AGCUACCAUC CGCUGGAUUA UGGCUGAACG CCUCUAAGUC AGAAUCCAUG CUAGAACGC GGUGAUUUCU UUGCUCCACA CAAUAUAGAU GGAUACGAAU AAGGCGUCCU UGUGGCGUCG CUGAACCAUA GCAGGCUAGC AACGGUGCA CUUGGCGGAA AGGCCUUGGG UGCUUGCUGG CGAAUUGCAA UGUCAUUUUG CGUGGGGAUA AAUCAUUUGU A UACGACUU AGAUGUACAA CGGGGUAUUG UAAGCAGUAG AGUAGCCUUG UUGUUACGAU CUGCUGAGAU UAAGCCUUUG UU GUCUGAU UUGU

Macromolecule #39: 5s rRNA

• Macromolecule: Name: 5s rRNA / type: rna / ID: 39 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 38.951105 KDa

Sequence

String:

GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

GENBANK: GENBANK: CP008434.1

Macromolecule #40: 5.8 S rRNA

• Macromolecule: Name: 5.8 S rRNA / type: rna / ID: 40 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 50.682922 KDa

Sequence

String:

AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

GENBANK: GENBANK: MG214542.1

Macromolecule #82: TSV IRES

• Macromolecule: Name: TSV IRES / type: rna / ID: 82 / Number of copies: 1
• Source (natural): Organism: Taura syndrome virus
• Molecular weight: Theoretical: 64.94948 KDa

Sequence

String:

AAACUCCAUG UAUUGGUUAC CCAUCUGCAU CGAAAACUCU CCGAACACUA GGUGCAGUAA GGCUUUCAUG GAGUGGUUUG CUAUUUAGC GUACGUGUAC CAUAGGCAGC CCCAAAAACA CGUGUGAGGA GAAAGUCCCA GUCACUUUGG GCAAAGUAGA C AGCCGCGC UUGCGUGGUG GGACUUAAUU AAUGCCUGCU AACC

GENBANK: GENBANK: AF277675.1

Macromolecule #83: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 83 / Number of copies: 217 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #84: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 84 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #85: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 85 / Number of copies: 1 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Macromolecule #86: (1S,3S,3aR,4S,4aR,7R,7aR,8aS)-8a-{[(6-deoxy-4-O-methyl-alpha-D-al...

• Macromolecule: Name: (1S,3S,3aR,4S,4aR,7R,7aR,8aS)-8a-{[(6-deoxy-4-O-methyl-alpha-D-altropyranosyl)oxy]methyl}-4-formyl-7-methyl-3-(propan-2-yl)decahydro-1,4-methano-s-indacene-3a(1H)-carboxylate; type: ligand / ID: 86 / Number of copies: 1 / Formula: W9C
• Molecular weight: Theoretical: 493.61 Da

Chemical component information

ChemComp-W9C:
(1S,3S,3aR,4S,4aR,7R,7aR,8aS)-8a-{[(6-deoxy-4-O-methyl-alpha-D-altropyranosyl)oxy]methyl}-4-formyl-7-methyl-3-(propan-2-yl)decahydro-1,4-methano-s-indacene-3a(1H)-carboxylate

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Sugar embedding: Material: carbon
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

7mpi

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.87 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 47514
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD