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- EMDB-2858: Cryo-EM structure of yeast 80S ribosome calculated on Amazon's el... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-2858 | |||||||||
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Title | Cryo-EM structure of yeast 80S ribosome calculated on Amazon's elastic cloud computing network | |||||||||
![]() | 80S yeast ribosome reconstructed using Relion from published dataset EMPIAR ID 10002. | |||||||||
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![]() | Ribosome / yeast / Relion / Amazon | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.6 Å | |||||||||
![]() | Cianfrocco MA / Leschziner AE | |||||||||
![]() | ![]() Title: Low cost, high performance processing of single particle cryo-electron microscopy data in the cloud. Authors: Michael A Cianfrocco / Andres E Leschziner / ![]() Abstract: The advent of a new generation of electron microscopes and direct electron detectors has realized the potential of single particle cryo-electron microscopy (cryo-EM) as a technique to generate high- ...The advent of a new generation of electron microscopes and direct electron detectors has realized the potential of single particle cryo-electron microscopy (cryo-EM) as a technique to generate high-resolution structures. Calculating these structures requires high performance computing clusters, a resource that may be limiting to many likely cryo-EM users. To address this limitation and facilitate the spread of cryo-EM, we developed a publicly available 'off-the-shelf' computing environment on Amazon's elastic cloud computing infrastructure. This environment provides users with single particle cryo-EM software packages and the ability to create computing clusters with 16-480+ CPUs. We tested our computing environment using a publicly available 80S yeast ribosome dataset and estimate that laboratories could determine high-resolution cryo-EM structures for $50 to $1500 per structure within a timeframe comparable to local clusters. Our analysis shows that Amazon's cloud computing environment may offer a viable computing environment for cryo-EM. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 49.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 11.4 KB 11.4 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 8.5 KB | Display | ![]() |
Images | ![]() | 516.2 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | 80S yeast ribosome reconstructed using Relion from published dataset EMPIAR ID 10002. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.77 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : 80S yeast ribosome
Entire | Name: 80S yeast ribosome |
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Components |
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-Supramolecule #1000: 80S yeast ribosome
Supramolecule | Name: 80S yeast ribosome / type: sample / ID: 1000 Details: Micrographs were downloaded from EMPIAR dataset 10002, associated with the publication Bai et al. eLife (2013) Oligomeric state: ribosome-eukaryote / Number unique components: 1 |
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Molecular weight | Experimental: 4.2 MDa / Theoretical: 4.2 MDa |
-Supramolecule #1: 80S ribosome
Supramolecule | Name: 80S ribosome / type: complex / ID: 1 Details: Micrographs were downloaded from EMPAIR database 10002, associated with Bai et al. eLife (2013) Recombinant expression: No / Ribosome-details: ribosome-eukaryote: ALL |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Experimental: 4.2 MDa / Theoretical: 4.2 MDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.3 mg/mL |
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Buffer | pH: 7.45 Details: 3mM Hepes-KOH, 6.6 mM Tris-acetate pH 7.2, 3 mM NH4Cl, 6.6 mM NH4-acetate, 48 mM K-acetate, 4 mM Mg-acetate, 2.4 mM DTT |
Grid | Details: Quantifoil grids (2/2) with 3 nm thin carbon on top |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 90 K / Instrument: FEI VITROBOT MARK II Details: Micrographs were downloaded from EMPIAR database 10002, associated with Bai et al. eLife (2013) Method: Blot 2.5 seconds before plunging |
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Electron microscopy
Microscope | FEI POLARA 300 |
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Temperature | Min: 80 K / Max: 90 K / Average: 85 K |
Alignment procedure | Legacy - Astigmatism: Objective lens astigmatism was corrected at 59,000 times magnification |
Details | Micrographs were downloaded from EMPIAR 10002 |
Date | Jul 15, 2012 |
Image recording | Category: CCD / Film or detector model: FEI FALCON I (4k x 4k) / Digitization - Sampling interval: 14 µm / Number real images: 200 / Average electron dose: 16 e/Å2 Details: Every image is the average of sixteen frames recorded by the direct electron detector |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Calibrated magnification: 79096 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2 mm / Nominal defocus max: 3.44 µm / Nominal defocus min: 1.191 µm |
Sample stage | Specimen holder model: GATAN LIQUID NITROGEN |
Experimental equipment | ![]() Model: Tecnai Polara / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Initial model | PDB ID: ![]() 3u5b |
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Software | Name: ![]() |
Details | Each PDB model was docked using "Fit in map" in Chimera |
Refinement | Space: REAL / Protocol: RIGID BODY FIT |
-Atomic model buiding 2
Initial model | PDB ID: ![]() 3u5c |
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Software | Name: ![]() |
Details | Each PDB model was docked using "Fit in map" in Chimera |
Refinement | Space: REAL / Protocol: RIGID BODY FIT |
-Atomic model buiding 3
Initial model | PDB ID: ![]() 3u5d |
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Software | Name: ![]() |
Details | Each PDB model was docked using "Fit in map" in Chimera |
Refinement | Space: REAL / Protocol: RIGID BODY FIT |
-Atomic model buiding 4
Initial model | PDB ID: ![]() 3u5e |
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Software | Name: ![]() |
Details | Each PDB model was docked using "Fit in map" in Chimera |
Refinement | Space: REAL / Protocol: RIGID BODY FIT |