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- EMDB-28281: Subtomogram average of the T4SS of Coxiella Burnetii at pH 4.75 -
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Open data
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Basic information
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Title | Subtomogram average of the T4SS of Coxiella Burnetii at pH 4.75 | |||||||||
![]() | T4SS of Coxiella burnetii at pH 4.75 | |||||||||
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![]() | Secretion system / MEMBRANE PROTEIN | |||||||||
Biological species | ![]() | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 36.0 Å | |||||||||
![]() | Kaplan M / Ghosal D | |||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: subtomogram average of the T4SS of Coxiella Burnetii at pH 4.75 Authors: Kaplan M / Shepherd DC / Vankadari N / Kim KW / Larson CL / Przemyslaw D / Beare PA / Krzymowski E / Heinzen AH / Jensen GJ / Ghosal D | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 547.5 KB | ![]() | |
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Header (meta data) | ![]() ![]() | 11.7 KB 11.7 KB | Display Display | ![]() |
Images | ![]() | 53.8 KB | ||
Filedesc metadata | ![]() | 3.7 KB | ||
Others | ![]() ![]() | 506.2 KB 764.2 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 576 KB | Display | ![]() |
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Full document | ![]() | 575.5 KB | Display | |
Data in XML | ![]() | 8.1 KB | Display | |
Data in CIF | ![]() | 9.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | T4SS of Coxiella burnetii at pH 4.75 | ||||||||||||||||||||
Voxel size | X=Y=Z: 8.073 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Even half map
File | emd_28281_half_map_1.map | ||||||||||||
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Annotation | Even half map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Odd half map
File | emd_28281_half_map_2.map | ||||||||||||
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Annotation | Odd half map | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : T4SS of Coxiella burnetii at pH 4.75
Entire | Name: T4SS of Coxiella burnetii at pH 4.75 |
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Components |
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-Supramolecule #1: T4SS of Coxiella burnetii at pH 4.75
Supramolecule | Name: T4SS of Coxiella burnetii at pH 4.75 / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | cell |
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Sample preparation
Buffer | pH: 4.75 |
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Vitrification | Cryogen name: ETHANE-PROPANE |
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Electron microscopy
Microscope | FEI POLARA 300 |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 1.625 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 8.0 µm / Nominal defocus min: 6.0 µm |
Experimental equipment | ![]() Model: Tecnai Polara / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Applied symmetry - Point group: C13 (13 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 36.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 414 |
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Extraction | Number tomograms: 69 / Number images used: 414 |
Final angle assignment | Type: NOT APPLICABLE |