+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-27742 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Compressed tail sheath of P1 Bacteriophage | |||||||||
Map data | ||||||||||
Sample |
| |||||||||
Keywords | phage tail sheath / helical / cryo-TEM / VIRAL PROTEIN | |||||||||
Biological species | Escherichia phage P1 (virus) | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 3.5 Å | |||||||||
Authors | Sen A / Bhatt V / Kim K / Bouchet-Marquis C | |||||||||
Funding support | United States, 1 items
| |||||||||
Citation | Journal: To Be Published Title: Compressed tail sheath of P1 Bacteriophage Authors: Sen A / Bhatt V / Kim K / Bouchet-Marquis C | |||||||||
History |
|
-Structure visualization
Supplemental images |
---|
-Downloads & links
-EMDB archive
Map data | emd_27742.map.gz | 336.5 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-27742-v30.xml emd-27742.xml | 11.2 KB 11.2 KB | Display Display | EMDB header |
Images | emd_27742.png | 309 KB | ||
Filedesc metadata | emd-27742.cif.gz | 3.7 KB | ||
Others | emd_27742_half_map_1.map.gz emd_27742_half_map_2.map.gz | 293.7 MB 293.7 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-27742 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-27742 | HTTPS FTP |
-Validation report
Summary document | emd_27742_validation.pdf.gz | 955.4 KB | Display | EMDB validaton report |
---|---|---|---|---|
Full document | emd_27742_full_validation.pdf.gz | 955 KB | Display | |
Data in XML | emd_27742_validation.xml.gz | 23.6 KB | Display | |
Data in CIF | emd_27742_validation.cif.gz | 28 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-27742 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-27742 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|
-Map
File | Download / File: emd_27742.map.gz / Format: CCP4 / Size: 1.3 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.69445 Å | ||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Half map: #2
File | emd_27742_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #1
File | emd_27742_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Sample components
-Entire : Escherichia phage P1
Entire | Name: Escherichia phage P1 (virus) |
---|---|
Components |
|
-Supramolecule #1: Escherichia phage P1
Supramolecule | Name: Escherichia phage P1 / type: virus / ID: 1 / Parent: 0 / NCBI-ID: 2886926 / Sci species name: Escherichia phage P1 / Virus type: VIRION / Virus isolate: OTHER / Virus enveloped: No / Virus empty: No |
---|
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | helical reconstruction |
Aggregation state | helical array |
-Sample preparation
Buffer | pH: 7.4 |
---|---|
Grid | Model: C-flat-1.2/1.3 / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | TFS GLACIOS |
---|---|
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 30.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm |
-Image processing
Final reconstruction | Applied symmetry - Helical parameters - Δz: 17.328 Å Applied symmetry - Helical parameters - Δ&Phi: 31.437 ° Applied symmetry - Helical parameters - Axial symmetry: C6 (6 fold cyclic) Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 217411 |
---|---|
Startup model | Type of model: OTHER |
Final angle assignment | Type: NOT APPLICABLE |