[English] 日本語
![](img/lk-miru.gif)
- EMDB-26815: Cryo-EM structure of the ribosome bound Bacteroides thetaiotaomic... -
+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Cryo-EM structure of the ribosome bound Bacteroides thetaiotaomicron EF-G2 | |||||||||
![]() | ||||||||||
![]() |
| |||||||||
![]() | Translation elongation factor / Bacteroides thetaiotaomicron EF-G2 / TRANSLATION | |||||||||
Function / homology | ![]() ribosome disassembly / translation elongation factor activity / GTPase activity / GTP binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / Resolution: 3.7 Å | |||||||||
![]() | Wang C / Han W / Groisman EA / Liu J | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: Cryo-EM structure of the ribosome-bound Bacteroides thetaiotaomicron EF-G2 Authors: Wang C / Han W / Groisman EA | |||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 498.5 KB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 14.2 KB 14.2 KB | Display Display | ![]() |
Images | ![]() | 69.9 KB | ||
Filedesc metadata | ![]() | 5.4 KB | ||
Others | ![]() ![]() | 47.9 MB 48 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 439.5 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 439 KB | Display | |
Data in XML | ![]() | 11.9 KB | Display | |
Data in CIF | ![]() | 13.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7uvpMC M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 1.068 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Half map: #2
File | emd_26815_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #1
File | emd_26815_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : ribosome-bound Bacteroides thetaiotaomicron EF-G2
Entire | Name: ribosome-bound Bacteroides thetaiotaomicron EF-G2 |
---|---|
Components |
|
-Supramolecule #1: ribosome-bound Bacteroides thetaiotaomicron EF-G2
Supramolecule | Name: ribosome-bound Bacteroides thetaiotaomicron EF-G2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
---|---|
Source (natural) | Organism: ![]() |
-Macromolecule #1: Tetracycline resistance protein TetQ
Macromolecule | Name: Tetracycline resistance protein TetQ / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() Strain: ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50 |
Molecular weight | Theoretical: 80.353859 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MKVYQTNEIK NIALLGSSGS GKTTLVEAML FESGVIKRRG SVAAKNTVSD YFPVEQEYGY SVFSTVLHVE WNNKKLNIID CPGSDDFVG STVTALNVTD TAIILLNGQY GVEVGTQNHF RYTEKLNKPV IFLVNQLDNE KCDYDNILEQ LKEAYGSKVV P IQYPIATG ...String: MKVYQTNEIK NIALLGSSGS GKTTLVEAML FESGVIKRRG SVAAKNTVSD YFPVEQEYGY SVFSTVLHVE WNNKKLNIID CPGSDDFVG STVTALNVTD TAIILLNGQY GVEVGTQNHF RYTEKLNKPV IFLVNQLDNE KCDYDNILEQ LKEAYGSKVV P IQYPIATG PGFNALIDVL LMKKYSWKPE GGAPVIEDIP AEEMDKAMEM HKALVEAAAE NDEGLMEKFF EQDSLTEDEM RE GIRKGLI ARGMFPVFCV CGGKDMGVRR LMEFLGNVVP FVSEMPKVEN TDGKEVAPDV NGPESLYFFK TSVEPHIGEV SYF KVMSGK VREGDDLLNA DRGSKERIAQ IYVVAGGNRV KVEELQAGDI GAAVKLKDVK TGNTLNGKDC DYKFNFIKYP NSKY SRAIK PVNEADVEKM MTILNRMREE DPTWVIEQSK ELKQTLVHGQ GEFHLRTLKW RLENNEKLQV KFEEPKIPYR ETITK AARA DYRHKKQSGG AGQFGEVHLI VEPYKEGMPV PDTYKFNGQE FKITVRGTEE IPLEWGGKLV FINSIVGGSI DARFLP AIM KGIMSRLEQG PLTGSYARDV RVIVYDGKMH PVDSNEISFM LAGRNAFSEA FKNAGPKILE PIYDVEVFVP SDRMGDV MG DLQGRRAMIM GMSSEKGFEK LVAKVPLKEM SSYSTALSSL TGGRASFIMK FASYELVPTD VQDKLIKDFE AKQTEE UniProtKB: Tetracycline resistance protein TetQ |
-Macromolecule #2: GUANOSINE-5'-TRIPHOSPHATE
Macromolecule | Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 2 / Number of copies: 1 / Formula: GTP |
---|---|
Molecular weight | Theoretical: 523.18 Da |
Chemical component information | ![]() ChemComp-GTP: |
-Macromolecule #3: MAGNESIUM ION
Macromolecule | Name: MAGNESIUM ION / type: ligand / ID: 3 / Number of copies: 1 / Formula: MG |
---|---|
Molecular weight | Theoretical: 24.305 Da |
-Macromolecule #4: water
Macromolecule | Name: water / type: ligand / ID: 4 / Number of copies: 1 / Formula: HOH |
---|---|
Molecular weight | Theoretical: 18.015 Da |
Chemical component information | ![]() ChemComp-HOH: |
-Experimental details
-Structure determination
![]() | single particle reconstruction |
---|---|
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.5 |
---|
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: DIRECT ELECTRON DE-64 (8k x 8k) / Average electron dose: 30.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.6 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
-
Image processing
Startup model | Type of model: NONE |
---|---|
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 210467 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |