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データを開く
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基本情報
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タイトル | Structure of Contracted C. elegans TMC-1 complex | |||||||||
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![]() | Complex / MEMBRANE PROTEIN | |||||||||
機能・相同性 | ![]() detection of stimulus involved in sensory perception / striated muscle dense body / sensory perception of chemical stimulus / mechanosensitive monoatomic ion channel activity / non-motile cilium / sodium channel activity / calcium ion homeostasis / monoatomic ion transmembrane transport / monoatomic ion channel activity / neuronal cell body ...detection of stimulus involved in sensory perception / striated muscle dense body / sensory perception of chemical stimulus / mechanosensitive monoatomic ion channel activity / non-motile cilium / sodium channel activity / calcium ion homeostasis / monoatomic ion transmembrane transport / monoatomic ion channel activity / neuronal cell body / calcium ion binding / magnesium ion binding / membrane / plasma membrane / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.09 Å | |||||||||
![]() | Jeong H / Clark S / Gouaux E | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structures of the TMC-1 complex illuminate mechanosensory transduction. 著者: Hanbin Jeong / Sarah Clark / April Goehring / Sepehr Dehghani-Ghahnaviyeh / Ali Rasouli / Emad Tajkhorshid / Eric Gouaux / ![]() 要旨: The initial step in the sensory transduction pathway underpinning hearing and balance in mammals involves the conversion of force into the gating of a mechanosensory transduction channel. Despite the ...The initial step in the sensory transduction pathway underpinning hearing and balance in mammals involves the conversion of force into the gating of a mechanosensory transduction channel. Despite the profound socioeconomic impacts of hearing disorders and the fundamental biological significance of understanding mechanosensory transduction, the composition, structure and mechanism of the mechanosensory transduction complex have remained poorly characterized. Here we report the single-particle cryo-electron microscopy structure of the native transmembrane channel-like protein 1 (TMC-1) mechanosensory transduction complex isolated from Caenorhabditis elegans. The two-fold symmetric complex is composed of two copies each of the pore-forming TMC-1 subunit, the calcium-binding protein CALM-1 and the transmembrane inner ear protein TMIE. CALM-1 makes extensive contacts with the cytoplasmic face of the TMC-1 subunits, whereas the single-pass TMIE subunits reside on the periphery of the complex, poised like the handles of an accordion. A subset of complexes additionally includes a single arrestin-like protein, arrestin domain protein (ARRD-6), bound to a CALM-1 subunit. Single-particle reconstructions and molecular dynamics simulations show how the mechanosensory transduction complex deforms the membrane bilayer and suggest crucial roles for lipid-protein interactions in the mechanism by which mechanical force is transduced to ion channel gating. | |||||||||
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構造の表示
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マップデータ | ![]() | 205.7 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 19.9 KB 19.9 KB | 表示 表示 | ![]() |
画像 | ![]() | 74.5 KB | ||
その他 | ![]() ![]() | 226.4 MB 226.4 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
EMDBのページ | ![]() ![]() |
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マップ
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ボクセルのサイズ | X=Y=Z: 0.839 Å | ||||||||||||||||||||
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: #2
ファイル | emd_26742_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_26742_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Native TMC-1 complex
+超分子 #1: Native TMC-1 complex
+分子 #1: Transmembrane channel-like protein 1
+分子 #2: CALMyrin (Calcium and Integrin Binding protein) homolog
+分子 #3: Transmembrane inner ear expressed protein
+分子 #4: CALCIUM ION
+分子 #5: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
+分子 #6: DODECANE
+分子 #7: HEXADECANE
+分子 #8: CHOLESTEROL
+分子 #9: 2-acetamido-2-deoxy-beta-D-glucopyranose
+分子 #10: 1,2-Distearoyl-sn-glycerophosphoethanolamine
+分子 #11: PALMITIC ACID
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 8.5 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.4 µm / 最小 デフォーカス(公称値): 1.0 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |