+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-26657 | ||||||||||||
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Title | 50S E. coli ribosome bound to streptogramins VM2 and VS1 | ||||||||||||
Map data | |||||||||||||
Sample |
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Keywords | E. coli ribosome / streptogramin A / streptogramin B / antibiotics / RIBOSOME | ||||||||||||
Biological species | Escherichia coli (E. coli) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.29 Å | ||||||||||||
Authors | Pellegrino J / Lee DJ | ||||||||||||
Funding support | United States, 3 items
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Citation | Journal: To Be Published Title: 50S E. coli ribosome bound to streptogramins VM2 and VS1 Authors: Pellegrino J / Lee DJ / Seiple IB / Fraser JS | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_26657.map.gz | 442 MB | EMDB map data format | |
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Header (meta data) | emd-26657-v30.xml emd-26657.xml | 11.7 KB 11.7 KB | Display Display | EMDB header |
Images | emd_26657.png | 164.6 KB | ||
Filedesc metadata | emd-26657.cif.gz | 3.6 KB | ||
Others | emd_26657_half_map_1.map.gz emd_26657_half_map_2.map.gz | 112.1 MB 112.1 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-26657 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-26657 | HTTPS FTP |
-Related structure data
EM raw data | EMPIAR-11024 (Title: E. coli 50S ribosome bound to compounds VM2 and VS1 / Data size: 1.3 TB Data #1: Unaligned movies of 50S ribosome bound to VM2 and VS1 [micrographs - single frame] Data #2: Aligned, motioncorrected micrographs of 50S ribosome bound to VM2 and VS1 [micrographs - multiframe]) |
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-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_26657.map.gz / Format: CCP4 / Size: 476.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.665 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_26657_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_26657_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : 50S E. coli ribosome bound to streptogramins VM2 and VS1
Entire | Name: 50S E. coli ribosome bound to streptogramins VM2 and VS1 |
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Components |
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-Supramolecule #1: 50S E. coli ribosome bound to streptogramins VM2 and VS1
Supramolecule | Name: 50S E. coli ribosome bound to streptogramins VM2 and VS1 type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Escherichia coli (E. coli) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 69.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.0 µm / Nominal defocus min: 0.4 µm / Nominal magnification: 130000 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.29 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 134924 |
Initial angle assignment | Type: PROJECTION MATCHING |
Final angle assignment | Type: PROJECTION MATCHING |