+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-26546 | ||||||||||||
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Title | Cryo-EM of self-assembled cannula CanA | ||||||||||||
Map data | Cryo-EM of self-assembled cannula CanA | ||||||||||||
Sample |
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Keywords | self-assemble / cannula / helical tube / donor strand / PROTEIN FIBRIL | ||||||||||||
Biological species | Pyrodictium abyssi (archaea) | ||||||||||||
Method | helical reconstruction / cryo EM / Resolution: 2.6 Å | ||||||||||||
Authors | Wang F / Miller JG / Egelman EH / Conticello VP | ||||||||||||
Funding support | United States, 3 items
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Citation | Journal: To Be Published Title: Cryo-EM of self-assembled cannula CanA Authors: Wang F / Miller JG / Egelman EH / Conticello VP | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_26546.map.gz | 92.4 MB | EMDB map data format | |
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Header (meta data) | emd-26546-v30.xml emd-26546.xml | 13.8 KB 13.8 KB | Display Display | EMDB header |
Images | emd_26546.png | 116.1 KB | ||
Filedesc metadata | emd-26546.cif.gz | 5 KB | ||
Others | emd_26546_half_map_1.map.gz emd_26546_half_map_2.map.gz | 318.6 MB 318.6 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-26546 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-26546 | HTTPS FTP |
-Validation report
Summary document | emd_26546_validation.pdf.gz | 1 MB | Display | EMDB validaton report |
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Full document | emd_26546_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | emd_26546_validation.xml.gz | 17.4 KB | Display | |
Data in CIF | emd_26546_validation.cif.gz | 20.7 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-26546 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-26546 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_26546.map.gz / Format: CCP4 / Size: 343 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | Cryo-EM of self-assembled cannula CanA | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.08 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: half-map A
File | emd_26546_half_map_1.map | ||||||||||||
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Annotation | half-map A | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: half-map B
File | emd_26546_half_map_2.map | ||||||||||||
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Annotation | half-map B | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : CanA
Entire | Name: CanA |
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Components |
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-Supramolecule #1: CanA
Supramolecule | Name: CanA / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Pyrodictium abyssi (archaea) |
-Macromolecule #1: CanA
Macromolecule | Name: CanA / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Pyrodictium abyssi (archaea) |
Molecular weight | Theoretical: 19.989492 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MTTQSPLNSF YATGTAQAVS EPIDVESHLG SITPAAGAQG SDDIGYAIVW IKDQVNDVKL KVTLANAEQL KPYFKYLQIQ ITSGYETNS TALGNFSETK AVISLDNPSA VIVLDKEDIA VLYPDKTGYT NTSIWVPGEP DKIIVYNETK PVAILNFKAF Y EAKEGMLF DSLPVIFNFQ VLQVG |
-Macromolecule #2: CALCIUM ION
Macromolecule | Name: CALCIUM ION / type: ligand / ID: 2 / Number of copies: 2 / Formula: CA |
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Molecular weight | Theoretical: 40.078 Da |
-Macromolecule #3: water
Macromolecule | Name: water / type: ligand / ID: 3 / Number of copies: 2 / Formula: HOH |
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Molecular weight | Theoretical: 18.015 Da |
Chemical component information | ChemComp-HOH: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | helical reconstruction |
Aggregation state | filament |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Applied symmetry - Helical parameters - Δz: 1.59 Å Applied symmetry - Helical parameters - Δ&Phi: -173.747 ° Applied symmetry - Helical parameters - Axial symmetry: C1 (asymmetric) Resolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 659059 |
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Startup model | Type of model: NONE |
Final angle assignment | Type: NOT APPLICABLE |