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- EMDB-2628: Structural similarity of secretins from Type II and Type III Secr... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-2628 | |||||||||
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Title | Structural similarity of secretins from Type II and Type III Secretion Systems | |||||||||
![]() | PulD | |||||||||
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![]() | bacterial secretion system / membrane protein / electron microscopy / Single Particle Analysis / secretin | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 8.2 Å | |||||||||
![]() | Tosi T / Estrozi LF / Job V / Guilvout I / Pugsley AP / Schoehn G / Dessen A | |||||||||
![]() | ![]() Title: Structural similarity of secretins from type II and type III secretion systems. Authors: Tommaso Tosi / Leandro F Estrozi / Viviana Job / Ingrid Guilvout / Anthony P Pugsley / Guy Schoehn / Andréa Dessen / ![]() ![]() Abstract: Secretins, the outer membrane components of several secretion systems in Gram-negative bacteria, assemble into channels that allow exoproteins to traverse the membrane. The membrane-inserted, ...Secretins, the outer membrane components of several secretion systems in Gram-negative bacteria, assemble into channels that allow exoproteins to traverse the membrane. The membrane-inserted, multimeric regions of PscC, the Pseudomonas aeruginosa type III secretion system secretin, and PulD, the Klebsiella oxytoca type II secretion system secretin, were purified after cell-free synthesis and their structures analyzed by single particle cryoelectron microscopy. Both homomultimeric, barrel-like structures display a "cup and saucer" architecture. The "saucer" region of both secretins is composed of two distinct rings, with that of PulD being less segmented than that of PscC. Both secretins have a central chamber that is occluded by a plug linked to the chamber walls through hairpin-like structures. Comparisons with published structures from other bacterial systems reveal that secretins have regions of local structural flexibility, probably reflecting their evolved functions in protein secretion and needle assembly. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 21.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 8.1 KB 8.1 KB | Display Display | ![]() |
Images | ![]() ![]() | 255.1 KB 255.1 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 208.6 KB | Display | ![]() |
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Full document | ![]() | 207.7 KB | Display | |
Data in XML | ![]() | 6.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | PulD | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.3355 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : PulD
Entire | Name: PulD |
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Components |
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-Supramolecule #1000: PulD
Supramolecule | Name: PulD / type: sample / ID: 1000 / Oligomeric state: dodecamer / Number unique components: 1 |
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Molecular weight | Experimental: 468.6 KDa / Theoretical: 468.6 KDa |
-Macromolecule #1: PulD
Macromolecule | Name: PulD / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Oligomeric state: dodecamer / Recombinant expression: Yes |
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Source (natural) | Organism: ![]() |
Molecular weight | Experimental: 468.6 KDa / Theoretical: 468.6 KDa |
Recombinant expression | Organism: Cell-free |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1.0 mg/mL |
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Grid | Details: 200 mesh coper quantifoil grid with thin carbon support |
Vitrification | Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI POLARA 300 |
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Date | Jan 1, 2012 |
Image recording | Category: CCD / Film or detector model: GATAN ULTRASCAN 4000 (4k x 4k) / Number real images: 1060 / Average electron dose: 20 e/Å2 / Bits/pixel: 8 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.0 mm / Nominal magnification: 59000 |
Sample stage | Specimen holder model: GATAN LIQUID NITROGEN |
Experimental equipment | ![]() Model: Tecnai Polara / Image courtesy: FEI Company |
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Image processing
Details | The particles were selected using an automatic selection program. |
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CTF correction | Details: Phase-flipping |
Final reconstruction | Applied symmetry - Point group: C12 (12 fold cyclic) / Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 8.2 Å / Resolution method: OTHER / Software - Name: Imagic, RIco, bsoft, ctffind3, FPM / Number images used: 63500 |