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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Cryo-EM map of HIV-1 1059 SOSIP in complex with 17b Fab | |||||||||
![]() | Single-particle cryo-EM map of HIV-1 1059 Env bound to 17b Fab | |||||||||
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![]() | HIV-1 / Env / bNAb / transmitted-founder / VIRAL PROTEIN | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.7 Å | |||||||||
![]() | Dam KA / Bjorkman PJ / Herschhorn A | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Conformational flexibility of HIV-1 Envelope glycoproteins modulates transmitted / founder sensitivity to broadly neutralizing antibodies Authors: Dam KA / Bjorkman PJ / Herschhorn A | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 15.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 7.4 KB 7.4 KB | Display Display | ![]() |
Images | ![]() | 118.3 KB | ||
Filedesc metadata | ![]() | 3.7 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 391.8 KB | Display | ![]() |
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Full document | ![]() | 391.3 KB | Display | |
Data in XML | ![]() | 6.7 KB | Display | |
Data in CIF | ![]() | 7.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Single-particle cryo-EM map of HIV-1 1059 Env bound to 17b Fab | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.832 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Complex of HIV-1 Env 1059 bound to 17b Fab
Entire | Name: Complex of HIV-1 Env 1059 bound to 17b Fab |
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Components |
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-Supramolecule #1: Complex of HIV-1 Env 1059 bound to 17b Fab
Supramolecule | Name: Complex of HIV-1 Env 1059 bound to 17b Fab / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 300 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 1.5 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 143940 |
Initial angle assignment | Type: RANDOM ASSIGNMENT |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |