[English] 日本語
![](img/lk-miru.gif)
- EMDB-26204: Ciliary 96-nm repeat unit from Salpingoeca rosetta (choanoflagell... -
+
Open data
-
Basic information
Entry | ![]() | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | Ciliary 96-nm repeat unit from Salpingoeca rosetta (choanoflagellate), generated via cryo-electron tomography and subtomogram averaging | |||||||||||||||
![]() | Averaged 96 nm repeat unit from Salpingoeca rosetta (choanoflagellate) cilia, centered between radial spokes 1 and 2. | |||||||||||||||
![]() |
| |||||||||||||||
![]() | cilia dynein microtubule radial spoke / STRUCTURAL PROTEIN | |||||||||||||||
Biological species | ![]() | |||||||||||||||
Method | electron tomography / cryo EM / Resolution: 22.0 Å | |||||||||||||||
![]() | Pinskey JM / Nicastro D | |||||||||||||||
Funding support | ![]()
| |||||||||||||||
![]() | ![]() Title: Ciliary 96-nm repeat unit from Salpingoeca rosetta (choanoflagellate), generated via cryo-electron tomography and subtomogram averaging. Authors: Pinskey JM / Nicastro D | |||||||||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 82.2 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 9.3 KB 9.3 KB | Display Display | ![]() |
Images | ![]() | 64.7 KB | ||
Filedesc metadata | ![]() | 3.9 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 538.3 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 537.8 KB | Display | |
Data in XML | ![]() | 4.9 KB | Display | |
Data in CIF | ![]() | 5.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Averaged 96 nm repeat unit from Salpingoeca rosetta (choanoflagellate) cilia, centered between radial spokes 1 and 2. | ||||||||||||||||||||
Voxel size | X=Y=Z: 3.151 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-
Sample components
-Entire : 96-nm repeat unit from Salpingoeca rosetta cilia
Entire | Name: 96-nm repeat unit from Salpingoeca rosetta cilia |
---|---|
Components |
|
-Supramolecule #1: 96-nm repeat unit from Salpingoeca rosetta cilia
Supramolecule | Name: 96-nm repeat unit from Salpingoeca rosetta cilia / type: organelle_or_cellular_component / ID: 1 / Parent: 0 Details: Contains doublet microtubule, dyneins, radial spokes, and nexin-dynein regulatory complex |
---|---|
Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | electron tomography |
Aggregation state | cell |
-
Sample preparation
Buffer | pH: 7 |
---|---|
Grid | Model: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE |
Cryo protectant | liquid ethane |
Sectioning | Other: NO SECTIONING |
Fiducial marker | Manufacturer: Millipore Sigma / Diameter: 10 nm |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Specialist optics | Phase plate: VOLTA PHASE PLATE |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 1.6 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 0.5 µm / Nominal defocus min: 0.5 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
-
Image processing
Final reconstruction | Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 22.0 Å / Resolution method: FSC 0.5 CUT-OFF / Number images used: 61 |
---|