+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-25675 | |||||||||
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Title | Structure of bacteriophage lambda tube protein V in C3 | |||||||||
Map data | Bacteriophage lambda tube protein V in C3 | |||||||||
Sample |
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Keywords | Virus / Phage / Lambda / Siphoviridae / Tail / Tube | |||||||||
Function / homology | Function and homology information virus tail, tube / symbiont genome ejection through host cell envelope, long flexible tail mechanism / viral tail assembly / host cell cytoplasm Similarity search - Function | |||||||||
Biological species | Escherichia virus Lambda | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.7 Å | |||||||||
Authors | Prokhorov NS / Yang Q / Catalano CE / Morais MC | |||||||||
Funding support | United States, 1 items
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Citation | Journal: To Be Published Title: Structure of bacteriophage lambda tube protein V in C6 Authors: Prokhorov NS / Yang Q / Catalano CE / Morais MC | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_25675.map.gz | 54.1 MB | EMDB map data format | |
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Header (meta data) | emd-25675-v30.xml emd-25675.xml | 13.4 KB 13.4 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_25675_fsc.xml | 11.6 KB | Display | FSC data file |
Images | emd_25675.png | 124.5 KB | ||
Others | emd_25675_half_map_1.map.gz emd_25675_half_map_2.map.gz | 58.8 MB 58.8 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-25675 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-25675 | HTTPS FTP |
-Related structure data
Related structure data | 7t4fMC 7t2eC C: citing same article (ref.) M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_25675.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | Bacteriophage lambda tube protein V in C3 | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.1 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Bacteriophage lambda tube protein V in C3
File | emd_25675_half_map_1.map | ||||||||||||
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Annotation | Bacteriophage lambda tube protein V in C3 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Bacteriophage lambda tube protein V in C3
File | emd_25675_half_map_2.map | ||||||||||||
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Annotation | Bacteriophage lambda tube protein V in C3 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Escherichia virus Lambda
Entire | Name: Escherichia virus Lambda |
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Components |
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-Supramolecule #1: Escherichia virus Lambda
Supramolecule | Name: Escherichia virus Lambda / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 10710 / Sci species name: Escherichia virus Lambda / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No |
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-Macromolecule #1: Tail tube protein
Macromolecule | Name: Tail tube protein / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: Escherichia virus Lambda |
Molecular weight | Theoretical: 25.700582 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: PVPNPTMPVK GAGTTLWVYK GSGDPYANPL SDVDWSRLAK VKDLTPGELT AESYDDSYLD DEDADWTATG QGQKSAGDTS FTLAWMPGE QGQQALLAWF NEGDTRAYKI RFPNGTVDVF RGWVSSIGKA VTAKEVITRT VKVTNVGRPS MAEDRSTVTA A TGMTVTPA ...String: PVPNPTMPVK GAGTTLWVYK GSGDPYANPL SDVDWSRLAK VKDLTPGELT AESYDDSYLD DEDADWTATG QGQKSAGDTS FTLAWMPGE QGQQALLAWF NEGDTRAYKI RFPNGTVDVF RGWVSSIGKA VTAKEVITRT VKVTNVGRPS MAEDRSTVTA A TGMTVTPA STSVVKGQST TLTVAFQPEG VTDKSFRAVS ADKTKATVSV SGMTITVNGV AAGKVNIPVV SGNGEFAAVA EI TVTAS |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 35.6 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |