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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Structure of bacteriophage lambda tube protein V in C3 | |||||||||
![]() | Bacteriophage lambda tube protein V in C3 | |||||||||
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![]() | Virus / Phage / Lambda / Siphoviridae / Tail / Tube | |||||||||
Function / homology | ![]() virus tail, tube / symbiont genome ejection through host cell envelope, long flexible tail mechanism / viral tail assembly / host cell cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.7 Å | |||||||||
![]() | Prokhorov NS / Yang Q | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of bacteriophage lambda tube protein V in C6 Authors: Prokhorov NS / Yang Q / Catalano CE / Morais MC | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 54.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.2 KB 13.2 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 11.6 KB | Display | ![]() |
Images | ![]() | 124.5 KB | ||
Filedesc metadata | ![]() | 5 KB | ||
Others | ![]() ![]() | 58.8 MB 58.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7t4fMC ![]() 7t2eC C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Bacteriophage lambda tube protein V in C3 | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.1 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Bacteriophage lambda tube protein V in C3
File | emd_25675_half_map_1.map | ||||||||||||
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Annotation | Bacteriophage lambda tube protein V in C3 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Bacteriophage lambda tube protein V in C3
File | emd_25675_half_map_2.map | ||||||||||||
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Annotation | Bacteriophage lambda tube protein V in C3 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Escherichia virus Lambda
Entire | Name: ![]() |
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Components |
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-Supramolecule #1: Escherichia virus Lambda
Supramolecule | Name: Escherichia virus Lambda / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 10710 / Sci species name: Escherichia virus Lambda / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No |
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-Macromolecule #1: Tail tube protein
Macromolecule | Name: Tail tube protein / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 25.700582 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: PVPNPTMPVK GAGTTLWVYK GSGDPYANPL SDVDWSRLAK VKDLTPGELT AESYDDSYLD DEDADWTATG QGQKSAGDTS FTLAWMPGE QGQQALLAWF NEGDTRAYKI RFPNGTVDVF RGWVSSIGKA VTAKEVITRT VKVTNVGRPS MAEDRSTVTA A TGMTVTPA ...String: PVPNPTMPVK GAGTTLWVYK GSGDPYANPL SDVDWSRLAK VKDLTPGELT AESYDDSYLD DEDADWTATG QGQKSAGDTS FTLAWMPGE QGQQALLAWF NEGDTRAYKI RFPNGTVDVF RGWVSSIGKA VTAKEVITRT VKVTNVGRPS MAEDRSTVTA A TGMTVTPA STSVVKGQST TLTVAFQPEG VTDKSFRAVS ADKTKATVSV SGMTITVNGV AAGKVNIPVV SGNGEFAAVA EI TVTAS UniProtKB: Tail tube protein |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 35.6 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |