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- EMDB-25656: Helical reconstruction of A92E HIV capsid in complex with CPSF6 c... -
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Open data
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Basic information
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Title | Helical reconstruction of A92E HIV capsid in complex with CPSF6 construct | |||||||||
![]() | Helical reconstruction of A92E HIV capsid in complex with CPSF6 construct | |||||||||
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Biological species | ![]() ![]() | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 7.4 Å | |||||||||
![]() | Iqbal N / Asturias F / Kvaratskhelia M | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Helical reconstruction of A92E HIV capsid in complex with CPSF6 construct Authors: Iqbal N / Asturias F / Kvaratskhelia M | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 21.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.1 KB 9.1 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.6 KB | Display | ![]() |
Images | ![]() | 164.8 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | Helical reconstruction of A92E HIV capsid in complex with CPSF6 construct | ||||||||||||||||||||
Voxel size | X=Y=Z: 2.8 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : HIV Capsid Protein Hexamer
Entire | Name: HIV Capsid Protein Hexamer |
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Components |
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-Supramolecule #1: HIV Capsid Protein Hexamer
Supramolecule | Name: HIV Capsid Protein Hexamer / type: complex / Chimera: Yes / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | helical reconstruction |
Aggregation state | 3D array |
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Sample preparation
Concentration | 3 mg/mL |
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Buffer | pH: 8 / Details: 1M NaCl, 50mM Tris pH 8, 0.2mM IP6 |
Grid | Model: C-flat / Pretreatment - Type: PLASMA CLEANING / Pretreatment - Time: 6 sec. |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TALOS ARCTICA |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4090 pixel / Number grids imaged: 2 / Number real images: 4706 / Average electron dose: 45.65 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Calibrated magnification: 28000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 28000 |
Sample stage | Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |