National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM094522
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM123655-03
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM051487
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM127018
米国
National Science Foundation (NSF, United States)
MCB-1617028
米国
National Science Foundation (NSF, United States)
MCB-1055017
米国
Howard Hughes Medical Institute (HHMI)
米国
引用
ジャーナル: Science / 年: 2022 タイトル: Structural and functional insight into regulation of kinesin-1 by microtubule-associated protein MAP7. 著者: Luke S Ferro / Qianglin Fang / Lisa Eshun-Wilson / Jonathan Fernandes / Amanda Jack / Daniel P Farrell / Mert Golcuk / Teun Huijben / Katelyn Costa / Mert Gur / Frank DiMaio / Eva Nogales / Ahmet Yildiz / 要旨: Microtubule (MT)-associated protein 7 (MAP7) is a required cofactor for kinesin-1-driven transport of intracellular cargoes. Using cryo-electron microscopy and single-molecule imaging, we ...Microtubule (MT)-associated protein 7 (MAP7) is a required cofactor for kinesin-1-driven transport of intracellular cargoes. Using cryo-electron microscopy and single-molecule imaging, we investigated how MAP7 binds MTs and facilitates kinesin-1 motility. The MT-binding domain (MTBD) of MAP7 bound MTs as an extended α helix between the protofilament ridge and the site of lateral contact. Unexpectedly, the MTBD partially overlapped with the binding site of kinesin-1 and inhibited its motility. However, by tethering kinesin-1 to the MT, the projection domain of MAP7 prevented dissociation of the motor and facilitated its binding to available neighboring sites. The inhibitory effect of the MTBD dominated as MTs became saturated with MAP7. Our results reveal biphasic regulation of kinesin-1 by MAP7 in the context of their competitive binding to MTs.