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- EMDB-25096: Electron cryo-tomography of CHIKV assembly intermediates in human... -
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Open data
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Basic information
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Title | Electron cryo-tomography of CHIKV assembly intermediates in human U2OS cells | |||||||||
![]() | CHIKV assembly/budding intermediate from virus-infected human U2OS cells | |||||||||
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![]() | alphavirus / enveloped virus / virus assembly and budding / cryogenic electron tomography / subtomogram averaging / VIRUS | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 23.0 Å | |||||||||
![]() | Chmielewski D / Schmid MF / Simmons G / Jin J / Chiu W | |||||||||
Funding support | ![]()
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![]() | ![]() Title: In situ Alphavirus Assembly and Budding Mechanism Revealed by Cellular CryoET Authors: Chmielewski D / Schmid M / Simmons G / Jin J / Chiu W | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 43.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 8.7 KB 8.7 KB | Display Display | ![]() |
Images | ![]() | 83.4 KB | ||
Filedesc metadata | ![]() | 3.7 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 391.5 KB | Display | ![]() |
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Full document | ![]() | 391.1 KB | Display | |
Data in XML | ![]() | 7.2 KB | Display | |
Data in CIF | ![]() | 8.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | C: citing same article ( |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | CHIKV assembly/budding intermediate from virus-infected human U2OS cells | ||||||||||||||||||||
Voxel size | X=Y=Z: 3.54 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Chikungunya virus
Entire | Name: ![]() ![]() |
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Components |
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-Supramolecule #1: Chikungunya virus
Supramolecule | Name: Chikungunya virus / type: virus / ID: 1 / Parent: 0 Details: CHIKV assembly/budding intermediate from virus-infected human U2OS cells NCBI-ID: 37124 / Sci species name: Chikungunya virus / Sci species strain: 181/clone 25 / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: Yes / Virus empty: No |
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Molecular weight | Theoretical: 65 MDa |
Virus shell | Shell ID: 1 / Diameter: 700.0 Å / T number (triangulation number): 4 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | TFS TALOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 1.8 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
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Image processing
Final reconstruction | Applied symmetry - Point group: C5 (5 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 23.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 75 |
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Extraction | Number tomograms: 185 / Number images used: 75 |
Final angle assignment | Type: PROJECTION MATCHING |